2001 Fiscal Year Final Research Report Summary
Pharmacological Study on Neural Regulation of Bone Metabolism
| Project/Area Number |
11671861
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Aichi-Gakuin University |
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Principal Investigator |
TOGARI Akifumi Aichi-Gakuin University, School of Dentistry, Department of Pharmacology, Professor, 歯学部, 教授 (80126325)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Ayami Aichi-Gakuin University, School of Dentistry, Department of Pharmacology, Research Associate, 歯学部, 助手 (70301629)
MOGI Makio Aichi-Gakuin University, School of Dentistry, Department of Pharmacology, Assistant Professor, 歯学部, 講師 (00174334)
ARAI Michitsugu Aichi-Gakuin University, School of Dentistry, Department of Pharmacology, Assistant Professor, 歯学部, 講師 (20097538)
HIRUKAWA Koji Aichi-Gakuin University, School of Dentistry, Department of Pharmacology, Research Associate, 歯学部, 助手 (60340147)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Osteoblast / Osteoclast / Osteoclastogenesis / Sympathetic neurons / Axon guidance molecules / RANKL / OPG |
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| Research Abstract |
In the present study, analysis of mRNA expression using RT-PCR demonstrated that human osteoblastic and osteoclastic cells are equipped with adrenergic receptors and neuropeptide receptors and constitutively express diffusible axon guidance molecules, suggesting that the extension of axons of sympathetic and peripheral sensory neurons to these cells is required for the dynamic neural regulation of local bone metabolism. Adrenergic stimulation was demonstrated to modulate the expression of IL-6, IL-11, cyclooxygenase-II, ODF/OPGL/TRANCE/RANKL(ODF/RANKL) and OCIF/OPG in osteoblasts/stromal cells. These molecules are well known to be involved in osteoclastogenesis. Especially, ODF/RANKL and OCIF/OPG are noticed as direct molecules modulating osteoclastogenesis. To elucidate the adrenergic regulation of osteoclastogenesis, we examined the effects of epinephrine (Epi), isoprenaline (Isp) and phenylephrine (Phe) on the formation of tartarate-resistant acid phosphatase(TRACP)-positive multinucleated cell (MNC) from mouse bone marrow cells. TRACP-positive MNC formation were stimulated by Isp or Epi, but not Phe. The MNC formation induced by Isp was not observed in the presence of OCIF/OPG, suggesting the importance of physiological balance between ODF/RANKL and OCIF/OPG productions for osteoclastogenesis. From these results, it may be suggested that the activity of sympathetic nerves has a significant effect on osteoclastogenesis by modulating ODF and OCIF expression in bone marrow cells.
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[Publications] Togari, A., Mogi, M, Arai, M., Yamamoto, S. and Koshihara, Y.: "Expression of mRNAs for axon guidance molecules, such as semaphorin-III, netrins and neurotrophins, in human osteoblasts and osteoclasts."Brain Research. 878. 204-209 (2000)
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[Publications] Togari, A., Arai, M., Mogi, M., Kondo, A., Tsuboi,T., Takeuchi, T., Yamamoto, S. and Koshihara, Y.: "Expression of mRNAs for neuropeptides receptors, adrenergic receptors, neurotrophins and semaphorin III in human osteoblasts and osteoclasts."Frontiers of the Mechanisms of Memory and Dementia (Elsevier). 163-164 (2000)
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「研究成果報告書概要(欧文)」より
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