2000 Fiscal Year Final Research Report Summary
Identification and characterization of the stem cells in human oral epithelia
Project/Area Number |
11671874
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
病態科学系歯学(含放射線系歯学)
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Research Institution | Yokohama City University |
Principal Investigator |
KAWABE Ryoichi Yokohama City University, School of Medicine, Associate Professor, 医学部, 助教授 (00195140)
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Project Period (FY) |
1999 – 2000
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Keywords | Oral mucosa / Stem cell / Oral Lichen planus / Immunohistochemistry |
Research Abstract |
In the present study, I assessed human oral mucosa with histochemistory for expression of 75kd nerve growth factor low-affinity receptor (NGFR) by means of its monoclonal antibody. NGFR is an additional component of the gp140 trk receptor complex, which is recognized as a marker of stem cells. I examined mucosal tissues which were embedded in OCT compound and frozen, and were fixed with formalin and paraffin embedded. NGFR positive cells are localized in the basal layer but not in the suprabasal layer of normal oral mucosa. The cells with high expression of NGFR clustered in restricted regions of the basal layer facing the basement membrane. In tongue and gingival, NGFR rich cells were founded adjacent to the tips of the dermal papillae, or at the tips of the deep rate ridges. In buccal mucosa, patches of the basal cells with high-level expression of NGFR were regularly interspersed with stretches of the basal cells of lower expression. Oral lichen planus that was a chronic inflammatory oral mucosal disease showed increased cell proliferation activity and the increased expression of the p53 and p21Cip1 in the basal and parabasal layers could reflect cell injury. Localization of NGFR rich cells in oral lichen planus was the same as normal mucosa. But a few of these cells in oral lichen planus showed Ki-67 positive, which were rarely seen in normal mucosa. This study revealed that expression of NGFR could clarify the strata of the basal cells in human oral epithelia.
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