Isolation of a novel tumor suppressor gene on the Chromosome 7 associated with oral squamous cell carcinoma.

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 11671976
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: CHIBA UNIVERSITY
• Principal Investigator: UZAWA Katsuhiro Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (30302558)
• Co-Investigator(Kenkyū-buntansha): SHIRASAWA Hiroshi Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (00216194) ; SUZUKI Nobuo Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (90111426) ; TANZAWA Hideki Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (50236775) ; WATANABE Toshihide Chiba University, Graduate School of Medicine, Assistant, 大学院・医学研究院, 助手 (10301095)
• Project Period (FY): 1999 – 2001
• Keywords: 7q31.1-31.2 / LOH / tumor suppressor gene / oral cancer

Research Abstract

To evaluate the role of chromosome 7 deletions in oral squamous cell carcinoma (SCC) progression and to define the precise localization of putative tumor suppressor genes, we studied tumors from 35 unrelated patients with oral SCC by the polymerase chain reaction (PCR)-loss of heterozygosity (LOH) assay, using 14 different polymorphic loci. Chromosome 7 allelic losses (LOH) were observed in 55.9% at one or more loci. Among the loci tested, frequent LOH was restricted at D7S522 on chromosome 7q31,1, which was measured within 1 cM. These results indicate that LOH of 7q is a common event in oncogenesis and/or progression of oral SCC, and also suggest the existence of a new suppressor gene near D7S522 locus, which may play a role in these events. To identify the candidate gene for the tumor suppressor gene near D7S522 locus, which may play a role in lymph node metastasis, differential display method and representational difference analysis, using tumors with and without LOH at this locus. Two DNA fragments was isolated and confirmed the presence on chromosome 7q. The sequences of the DNA fragments were detected and they were considered as a part of a putative tumor suppressor gene involved in the carcinogenesis and development of oral squamous cell carcinoma.