The investigation of mechanisms between PDT and metastatic effect of oral cancer

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 11672026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Aichi-Gakuin University
• Principal Investigator: YOSHIDA Kenji 1st Dept. of OMFS, Aichi-Gakuin University, Professor, 歯学部・口腔外科学第1講座・特殊診療科 教授 (40183701)
• Co-Investigator(Kenkyū-buntansha): KURITA Kenichi 1st Dept. of OMFS, Aichi-Gakuin University, Professor, 歯学部・口腔外科学第1講座, 教授 (40133483)
• Project Period (FY): 1999 – 2001
• Keywords: photodynamic therapy / PDT / metastasis

Research Abstract

Photodynamic therapy (PDT) is expected as a new laser therapy for treating the oral cancer. However the machanisms of metastastatic effect when PDT is conducted is unknown. This study was conducted to clarify the machanisms of metastasis when PDT is conducted.
Differentiated squamous cell carcinoma cell line (RSC3-E2) and undifferentiated tumor cell line (RSC3-LM) with different metastatic potential that established from a 4-nitroquinoline N-oxide (4NQO)-induced differentiated SCC in F344 rat were used for undergoing PDT using light (Wavelength 530〜540nm) and Photofrin^<TM> (hematoporphyrin derivative. Fibroblast cell line derived from mice was used as cntrol. Apoptosis indication was counted for estimating the metastatic influences. Apoptosis was not observed at RSC3-E2 and RSC3-LM cell lines when hematoporphyrin derivative was administered alone. However apoptosis was resisted at RSC3-E2 cell lines as well as control when PDT was underwent together with light source. These results suggested that metastasis would be promote on the conditions of PDT at this cell lines model.