2001 Fiscal Year Final Research Report Summary
Acquisition of pharmacologically active compounds from plant materials using ion-pair HPLC.
| Project/Area Number |
11672130
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
MARIYASU Masataka Kobe Pharmaceutical University, Natural Medical Chemistry, Professor, 薬学部, 教授 (40102107)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIYAMA Yumi Kobe Pharmaceutical University, Natural Medical Chemistry, Associate, 薬学部, 助手 (30248116)
ICHIMARU Momoyo Kobe Pharmaceutical University, Natural Medical Chemistry, Associate Professor, 薬学部, 講師 (00175259)
IWASA Kinuko Kobe Pharmaceutical University, Natural Medical Chemistry, Associate Professor, 薬学部, 助教授 (30068340)
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| Project Period (FY) |
1999 – 2001
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| Keywords | ion-pair HPLC / alkaloid / Monodora junodii / Xylopia parviflora / Monanthotaxis fornicata / Annonaceae plants / flavonoid / antitumor activity |
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| Research Abstract |
A rapid and convenient method was developed to purify alkaloids from plant materials by the combination ofion-pair extraction and ion-pair HPLC using sodium perchlorate as an ion-pair reagent. Alkaloids form neutraland relatively hydrophobic ion-pair with perchlorate anion. The resulting ion-pairs are extractable with someorganic solvents, and were excellently separated by means of ion-pair HPLC where perchlorate was used again asan ion-pair reagent. By these methods, we purified many alkaloids from plant materials. From threeAnnonaceous medicinal plants, Monodora junodii, Xylopia parviflora, and Monanthotaxis fornicata, manyisoquinoline alkaloids (benzylisoquinolines, aporphines, protoberberines, pseudoprotoberberines and simpleisoquinolines) including five new compounds were purified. The pharmacological activity of isolatedcompounds were examined, and the following results were obtained : (1) Pseudoprotoberberine alkaloids havestrong antimalarial activity, and among them the activit
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y of pseudocoptisine and peseudoberberine is the strongest. (2) Aporphine alkloaids having methylendioxy substituent in 1 - and 2-positions exhibit strong fungicidal activity. (3) Several benzylisoquinoline alkaloids in Monanthotaxis fornicata show growth inhibition against humanleukemia cell line, HL-60. (4) Alkaloidal fraction of Xylopia parviflora have strong analgesic action, andquaternary alkaloidal fraction has stronger activity than tertiary alkaloidal fraction.
The HPLC solvent system devised in this work also can be applied for acidic substances. Flavonoids were excellently separated by the same solvent system, where separation was achieved as ion-suppression mode. Flavonoids were purified from Japanese, Chinese and Kenyan medicinal plants. The pharmacological activity (anti-oxidant and antitumor activity) of isolated compounds was examined. A few flavonoids (vitexicarpine from Vitex rotandifolia and pachypodol from Macarisia pyramidatd) show very strong growth inhibition against human leukemia cell line, HL-60.f Less
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