1999 Fiscal Year Annual Research Report

細胞悪性化に関わるCD98シグナル伝達系の解析

Research Project

Project/Area Number	11672157
Research Institution	Tohoku University
Principal Investigator	益子高東北大学, 大学院・薬学研究科, 助教授 (30157200)
Co-Investigator(Kenkyū-buntansha)	榎本武美東北大学, 大学院・薬学研究科, 教授 (80107383)
Keywords	モノクローナル抗体(mAB) / CD98 / CD98hc / CD98lc / トロポミオシン / グルタミン酸トランスポーター / EAAC1 / 細胞悪性化
Research Abstract	細胞悪性化に関わるCD98シグナル伝達系の解析を目的として、ヒトCD98hc cDNAとラットCD98hc cDNAを、各々、マウス線維芽細胞NIH3T3とBalb3T3にトランスフェクトし、細胞悪性化形質を調べた。また、CD98hcと結合する可能性のある新規アミノ酸トランスポーターのクローン化、新規CD98lcに対するモノクローナル抗体(mAb)の作製と対応抗原の性状解析を行った。 1、CD98hcの導入によりマウス線維芽細胞は軟寒天中コロニー形成、ヌードマウスでの腫瘍形成などの悪性化形質を示した。 2、悪性形質転換には導入CD98hcと内因性CD98lcとの結合が必要だった。また、CD98hcの103番目のシステインがCD98lcとの結合に用いられていた。 3、CD98hcとの結合が示唆されているグルタミン酸受容体EAAC1を解析する過程で、欠失型EAAC1を同定、クローン化した。 4、新規CD98lcに対するmAbを作製し、この1cがhc同様、リンパ球活性化や癌化に伴って発現増強することを見出した。 5、CD98隣接分子としてアクチン結合性のトロポミオシンを同定した。

Research Products
(7 results)

All Other

All Publications (7 results)

[Publications] Matsumoto Y.: "ldentification of truncated human glutamate transporter"Tohoku J. Exp. Med.. 187. 173-182 (1999)
[Publications] Hara K.: "Malianant transformation of NIH3T3 cells by overexpression of early lymphocyte activation antigen CD98"Biochem. Biophys. Res. Commun.. 262. 720-725 (1999)
[Publications] Matsumoto Y.: "ldentification and lmmunological Characterization of a Novel 40-kDa Protein Linked to CD98 Antigen"Cell Struct. Funct.. 24. 217-226 (1999)
[Publications] Shiratori M: "Detection by epitope-defined monoclonal antibodies of Werner DNA helicases in the nucleoplasm and their upregulation by cell transformation and immortalization"J Cell Biol.. 144. 1-9 (1999)
[Publications] Shishido T.: "Colocalization of GP125/CD98 with tropomyosin isoforms at the cell-cell adhesion boundary"J. Biochem.. (in press). (2000)
[Publications] Shishido T.: "Transformation of Balb3T3 cells caused by over-expression of rat CD98 heavy chain (hc) recquires its association with light chain : Missense mutation in a cysteine residue of CD98hc-eliminates its transforming activity"Int. J. Cancer. GD(in press). (2000)
[Publications] Onoda F.: "Mutat. Res.(in press)"Elevation of the sister chromatid exchange in Saccharomyces cerevisiae sgs1 disruptants and the relevance of the disruptants as a system to evaluate mutations in Bloom's syndrome gene. (2000)

1999 Fiscal Year Annual Research Report

細胞悪性化に関わるCD98シグナル伝達系の解析

Principal Investigator

益子 高 東北大学, 大学院・薬学研究科, 助教授 (30157200)

Research Products

[Publications] Matsumoto Y.: "ldentification of truncated human glutamate transporter"Tohoku J. Exp. Med.. 187. 173-182 (1999)

[Publications] Hara K.: "Malianant transformation of NIH3T3 cells by overexpression of early lymphocyte activation antigen CD98"Biochem. Biophys. Res. Commun.. 262. 720-725 (1999)

[Publications] Matsumoto Y.: "ldentification and lmmunological Characterization of a Novel 40-kDa Protein Linked to CD98 Antigen"Cell Struct. Funct.. 24. 217-226 (1999)

[Publications] Shiratori M: "Detection by epitope-defined monoclonal antibodies of Werner DNA helicases in the nucleoplasm and their upregulation by cell transformation and immortalization"J Cell Biol.. 144. 1-9 (1999)

[Publications] Shishido T.: "Colocalization of GP125/CD98 with tropomyosin isoforms at the cell-cell adhesion boundary"J. Biochem.. (in press). (2000)

[Publications] Shishido T.: "Transformation of Balb3T3 cells caused by over-expression of rat CD98 heavy chain (hc) recquires its association with light chain : Missense mutation in a cysteine residue of CD98hc-eliminates its transforming activity"Int. J. Cancer. GD(in press). (2000)

[Publications] Onoda F.: "Mutat. Res.(in press)"Elevation of the sister chromatid exchange in Saccharomyces cerevisiae sgs1 disruptants and the relevance of the disruptants as a system to evaluate mutations in Bloom's syndrome gene. (2000)

益子高東北大学, 大学院・薬学研究科, 助教授 (30157200)