2001 Fiscal Year Final Research Report Summary
Biochemical and molecular mechanisms of beneficial effects for human health by phytoestrogens
Project/Area Number |
11672235
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Environmental pharmacy
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Research Institution | National Institute of Health Sciences |
Principal Investigator |
OZAWA Shogo National Institute of Health Sciences, Section chief, 薬理部, 室長 (20185581)
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Project Period (FY) |
1999 – 2001
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Keywords | Estrogenicity / Human / Drug Metabolizing enzymes / Sulfotransferase / detoxification / gene expression / estrogen responsiveness / phytoestrogens |
Research Abstract |
In this study, estrogenicity of bisphenol A and phytoestrogens such as genistein was compared in human breast cancer MCF-7 cells using so called E-screen assay. We also established induction of expression of pS2 gene, an estrogen-resposive gene, after exposure of MCF-7 cells to chemicals with estrogenicity. Estrogenic activities of daidzein, naringenin, kaempfenol were weaker than that of genistein. For detoxification pathway of estrogenic compounds, sulfoconjugation of bisphenol A and phytoestrogens was catalyzed by human hepatic microsomes. On the basis of Km values, affinity of bisphenol A to sulfotransferase seemed to be higher than that of phytoestrogens. A form of thermostable phenol-sulfating sulfotransferase, ST1A3, was identified as a bisphenol A-sulfating sulfotransferase. Difference in the mechanisms of estrogenicity between phytoestrogens and bisphenol A was not, however, achieved. It would be possible by analyses of gene expression after exposure of MCF-7 cells to phytoestrogens, and bisphenol A whose toxic action is suspected due to its estrogenic action.
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Research Products
(12 results)
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[Publications] S. Ozawa, M. Shimizu, T. Katoh, A. Miyajima, Y. Ohno, Y. Matsumoto, M. Fukuoka, Y.-M. Tang, N.P. Lang and F.F. Kadlubar: "Sulfating-activity and stability of cDNA-expressed allozymes of human phenol sulfotransferase, ST1A3*1 ((213)Arg) and ST1A3*2 ((213)His), both of which exist in Japanese as well as Caucasians"J Biochem (Tokyo). 126(2). 271-277 (1999)
Description
「研究成果報告書概要(欧文)」より
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