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2001 Fiscal Year Final Research Report Summary

Study of the regulation of granzyme B mRNA in CTL and its clinical significance

Research Project

Project/Area Number 11672298
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKobe University

Principal Investigator

RYO Ryukichi  Kobe University, School of Medicine, Professor, 医学部, 教授 (00159237)

Co-Investigator(Kenkyū-buntansha) HORIE Osamu  Kobe University, School of Medicine, Assistant Professor, 医学部, 助手 (50304118)
Project Period (FY) 1999 – 2000
Keywordsgranzyme B / perforin / apoptosis / HLA / ELISA / CTL / herpssvirus saimiri / minor histocompatibility antigen
Research Abstract

1. We reported that the patients with post-transfusion graft-versus-host disease (PT-GVHD) had a partial remission after administration of nafmostat mesilate, a serine protease inhibitor, thus indicating that granzyme(gr) plays an important role in organ failure in PT-GVHD.
2. Cytokine dependency of granzyme B(grB) mRNA expression was investigated using competitive RT-PCR. We found that the combination of IL-2 and IL-12 additively increased the expression of grB mRNA. It therefore seems possible that YT-u may have a different intracellular signal transudation system for cytokine-induced regulation of grB mRNA from that of normal NK cells.
3. Six kinds of monoclonal antibodies which recognized three different epitope were prepared using the recombinant grB protein produced with the GST fusion protein system. One step ELISA for grB was established using these antibodies. We clarified that this ELISA was two thousands as sensitive as an enzyme assay.
4. CTL clone was established in HLA- homozygous pairs and HLA-heterozygous pairs in vitro. To obtain a stable CTL clone, immortalization of Tcell was performed by infection with Herpesvirus saimiri. We found little difference between TNF production and the induction of grB gene expression in two CTLs which recognized major and minor histocompatibility antigen. We elucidated the importance of minor histocompatibility antigen in the induction of CTL clone.
5. To obtain an active grB and perforin, the experiment of the protein expression system with insect cells is now in progress.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Ryukichi Ryo, et al.: "Treatment of post-transfusion graft-versus-host disease with nafmostat mesilate, a serine protease inhibitor"Vox Sanguinis. 76. 241-246 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuyasu Saigo, Ryukichi Ryo: "Therapeutic Strategy for post-transfusion graft-vs-host disease"International Journal of Hematology. 69. 147-151 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Horie, Ryukichi Ryo: "Establishment of a lymphocyte clone expressing granzyme B and perforin mRNAs immortalized with Herpesvirus saimiri"Bulletin of Health Sciences Kobe. 16. 39-44 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Horie, Ryukichi Ryo: "Cytekine dependency of granzyme B mRNA expression in natural killer leukemic cell, YT-U"Bulletin of Health Sciences Kobe. 16. 57-62 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ryo R., Saigo K., Hashimoto M, Kohsaki M, et al: "Treatment of post-transfusion grant-versus-host disease with nafmostat mesilate, a serine protease inhibitor"Vox Sanguinis. 76-4. 241-246 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saigo K., Ryo R.: "Therapeutic strategy for post-transfuison graft-vs.-host disease"International Journal of Hematology. 69-3. 147-151 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horie O., Ryo R.: "Establishment of a lymphocyte clone expressing granzyme B and perforin mRNAs immortalized with Herpesvirus saimiri"Bulletin of Health Sciences Kobe. 16. 39-44 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horie O., Ryo R.: "Cytokine dependency of granzyme B mRNA expression in natural killer leukemic cell line, YT-u"Bulletin of Health Sciences Kobe. 16. 57-62 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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