2001 Fiscal Year Final Research Report Summary
Laboratory Investigation on Protein 1/ Clara Cell 10 kDa Protein
| Project/Area Number |
11672302
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Asahikawa Medical College (2000-2001)
Jichi Medical University (1999) |
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Principal Investigator |
ITOH Yoshihisa Asahikawa Medical College, Department of Medicine, Professor, 医学部, 教授 (20129026)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Protein 1 / Clara cell 10 kDa Protein / Uteroglobin / SNPs / Platelet |
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| Research Abstract |
Protein 1, also called as clara cell 10 kDa protein or uteroglobin, is nonglycoprotein with a molecular weight of 14 kDa playing anti-inflammatory function.
In the present term the following research work was successfully performed:
1. β2-microglobulin (β2-m) is unstable in acidic urine, but not protein 1. We could identify cathepsin D responsible for the degradation ofβ2-m. Stability of protein 1 can be explained by no cleavage sites present on its molecule. We will need the revaluation of the stability of all the urinary proteins for proper clinical use.
2. The G38A gene polymorphism on uteroglobin/P1 was investigated in normal individuals and patients with IgA nephropathy. The frequency of G38AA is 24 %, being 2 times higher in the diseases than normal. Furthermore we found decrease of the uteroglobin value in the patients' sera indicating that G38AA may be a predisposing factor for the diseases as a result of relative decrease of anti-inflammatory effects. We have newly discovered SNPs on P1 gene.
3. We recently found that pi exert an inhibitory effect on platelet aggregation by blocking influx of Ca. Further precise study is in progress to elucidate precise mechanisms.
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