2001 Fiscal Year Final Research Report Summary
Effects of dietary myo-inositol or phytate on the metabolic changes and toxicity due to environmental pollution substances
Project/Area Number |
11680133
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
食生活
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Research Institution | Hiroshima University |
Principal Investigator |
KATAYAMA Tetsuyuki Hiroshima University, Graduate School of Education, Associate professor, 大学院・教育学研究科, 助教授 (10233751)
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Project Period (FY) |
1999 – 2001
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Keywords | environmental pollution substances / DDT / myo-inositol / phytic acid / liver lipids / serum copper / drug-metabolizing enzymes / lipid peroxidation |
Research Abstract |
These studies were conducted to examine the effects of dietary carbohydrate [starch or sucrose] and myo-inositol or phytateon metabolic changes in rats fed 1,1,1-trichloro-2,2-bis (p-chlophenyl) ethane (DDT) (0.7g/kg diet). Dietary DDT enhanced serum and hepatic lipids and hepatic thiobarbituric acid reactive substances (TBA-RS), elevated hepatic activities of lipogenic enzymes such as malic enzymes (ME,EC 1.1.1.40), glucose-6-phosphate dehydrogenase (G6PD,EC 1.1.1.49) and fatty acid synthetase (FAS.EC 2.3.1.85), increased hepatic cytochrome P-450 content and the activities of drug-metabolizing enzymes such as aminopyrine demetylase, glutathion-s-transferases and 4-nitorophenol-UDP glucuronosyltransferases (4NP-UDGPT) and raised hepatic ascorbic acid and serum copper. Dietary sucrose promoted the increases in hepatic concentrations of total lipids, triglyceride and cholesterol, hepatic activities of ME, hepatic TBA-RS, cytochrome P-450 content and serum copper due to DDT feeding when compared with dietary starch. Dietary myo-inositol significantly depressed the rises in hepatic concentrations of total lipids and cholesterol and the activities of ME and G-6-P due to DDT feeding regardless of dietary carbohydrate. Dietary starch supplemented with myo-inositol potentiated the enhancements in hepatic activities of Phase II drug-metabolizing enzymes such as glutathion-s-transferases and 4NP-UDGPT due to DDT feeding. Dietary phytate had similar effects on the metabolic responses to DDT in rats. These results suggest that dietary starch and myo-inositol or phytate can protect DDT fed rats against an accumulation of hepatic lipids, which might be at least in part ascribed to the depression of hepatic lipogenesis. In addition, the present studies imply that the supplementation of myo-inositol or phytate to high starch diet might be improve the function of drug metabolizing enzymes exposed to DDT. During these studies, I reported that phytate may be an vitamin-like substance.
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Research Products
(6 results)