2000 Fiscal Year Final Research Report Summary
Functional characterization of TFIID subunits (TAFs) in Saccharomyces cerevisiae
| Project/Area Number |
11680680
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | NARA INSTITUTE OF SCIENCE AND TECHNOLOGY |
|---|
Principal Investigator |
KOKUBO Tetsuro Nara Institute of Science and Technology, Graduate School of Biological Sciences, Associate Professor, バイオサイエンス研究科, 助教授 (10271587)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Keywords | transcription / tanscriptional regulation / TFIID / TAF / general transcription factor / yeast / basal transcription / core promoter |
|---|
| Research Abstract |
The general transcription factor TFIID, which is composed of a TATA-binding protein (TBP) and a set of TBP-associated factors (TAFs), has been shown to be involved in both core promoter recognition and the transcriptional activation of eukaryotic genes. We isolated TAF145 temperature-sensitive mutants in yeast, in which transcription of the TUB2 gene is impaired at restrictive temperatures due to a defect in core promoter. We also showed in these mutants that the transcription of the RPS5 gene is impaired, mostly due to a defect in transcriptional activation rather than to a defect in core promoter recognition, although the latter is slightly affected as well. Surprisingly, the RPS5 core promoter can be activated by various activation domains fused to a GAL4 DNA binding domain (e.g. GAL4-RAP1, -VP16C, -EBNA2, and -GCN4), but not by the original upstream activating sequence (UAS) of the RPS5 gene. In addition, a heterologous CYC1 core promoter can be activated by RPS5-UAS at normal levels even in these mutants. These observations indicate that a distinct combination of core promoters and activators may exploit alternative activation pathways which vary in their requirement for TAF 145 function. In addition, a particular function of TAF 145 that is deleted in our mutants appears to be involved in both core promoter recognition and transcriptional activation
|
-
-
-
-
-
[Publications] Kobayashi, A., Miyake, T., Ohyama, Y., Kawaichi, M., and Kokubo, T.: "Mutations in the TATA binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae."Journal of Biological Chemistry. 276-1. 395-405 (2001)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Kotani, T., Banno, K-i., Ikura, M., Hinnebusch, A.G., Nakatani, Y., Kawaichi, M., _and Kokubo, T.: "A role of transcriptional activators as anti-repressors for the auto-inhibitory activity of TATA box binding of TFIID"Proc.Natl.Acad.Sci.USA. 97-13. 7178-7183
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Lim, C.R., Kimata, Y., Ohdate, H., Kokubo, T., Kikuchi, N., Horigome, T., and Kohno, T.: "The Saccharomyces cerevisiae RuvB-like protein, tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription"Journal of Biological Chemistry. 275-29. 22409-22417
Description
「研究成果報告書概要(欧文)」より
