2000 Fiscal Year Final Research Report Summary
MOLECULAR ANALYSIS OF CELL BARRIER ASSOCIATED PROTEIN BARMOTIN (7H6 ANTIGEN)
| Project/Area Number |
11680702
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
KOKAI Yasuo Department of Pathology Sapporo Medical University School of Medicine, 医学部, 助教授 (20178239)
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| Co-Investigator(Kenkyū-buntansha) |
TOBIOKA Hirotoshi Department of Pathology Sapporo Medical University School of Medicine, 医学部, 助手 (90291559)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Tight junction / barmotin / occludin / gap junction / connexin 32 / barrier / H.pylori / 細胞バリア |
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| Research Abstract |
Tight junctions localize uppermost of apposing epithelium and a class of endothelium. Tight junctions serve as a barrier for paracellular pathway and regulate traffics of ion, small molecules and cells. In the present projects, we studied molecular aspects of tight junction related proteins and its role in pathological conditions, such as bacterial gastritis by H.pylori. To perform this, we developed a model to study pathological role of tight junctions in human disease. Study using H.pylori adapted for murine stomach revealed tight junctions play an important role for these bacteria to colonize in the murine stomach. This observation suggests that up-regulation of paracellular barrier of gastric mucosa may prevent H.pylori colonization which leads to neutrophilic gastritis and strongly links to gastric carcinoma and lymphoma. We also elucidated that molecular regulation of tight junction related molecules by gap junction molecule, designated Cx32. This observation has opened up an opportunity to dissect how tight junctions develop and regulate as a member of junctional complex at the cell-cell contacts.
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[Publications] Takakuwa, R., Kokai, Y., Kojima, T., Akatsuka, T., Hirotoshi Tobioka, H., Sawada, N.& Mori, M.: "Uncoupling of gate and fence functions of MDCK cells by the actin depolymerizing reagent mycalolide B"Exp.Cell Res.. 257. 238-244 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Kojima, T., Kokai, Y., Chiba, H., Kuwahara, K., Mori, M., Mochizuki, M.& Sawada, N.: "Occludin and claudin-1 concentrate in the midbody of immortalized mouse hepatocytes during cell division"J.Histochem.Cytochem.. 49. 333-340 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Kojima, T., Kokai, Y., Chiba, H., Yamamoto, M., Mochizuki, Y.& Sawada, N.: "Cx32 but not Cx26 is associated with tight junctions in primary cultures of rat hepatocytes"Exp.Cell Res.. 263. 193-201 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Suzuki, K., Kokai, Y., Sawada, N., Takakuwa, R., Kuwahara, K., Isogai, E., Isogai, H.& Mori, M.: "SS1 Helicobacter pylori disrupts the paracellular barrier of the gastric mucosa and leads to neutrophilic gastritis in mice"Virchow Archives. (in press).
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「研究成果報告書概要(欧文)」より
