2000 Fiscal Year Final Research Report Summary
The Mechanism of the binary dicision of the lateral plate mesoderm
| Project/Area Number |
11680727
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | Kitasato-university |
|---|
Principal Investigator |
FUNAYAMA Noriko Kitasato-Univ.School of Science Assistant prof., 助手 (30276175)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Keywords | mesoderm subdivision / lateral plate mesoderm / Splanchnopleural mesoderm / Somatic mesoderm / lateral endoderm / lateral ectoderm / chick embryo / cell adhesion |
|---|
| Research Abstract |
Most triploblastic animals including vertebrates have a coelomic cavity that separates the outer and inner components of the body. The coelom is lined by two different tissue components, somatopleure and splanchnopleure, which are derived from the lateral plate region. Thus, the coelom is constructed by the binary decision during early specification of the lateral plate. In this report we studied the molecular mechanisms of this binary decision. We first demonstrate that the splitting of the lateral plate into the two cell sheets progresses in an antero posterior order and this progression is not coordinated with that of the somitic segmentation. By a series of embryological manipulations we found that a young splanchnic mesoderm is still competent to be respecified to the somatic mesoderm, and the ectoderm overlying the lateral plate is sufficient for this redirection. The lateral ectoderm is also required for maintenance of the somatic character of the mesoderm. Thus, the ectoderm plays at least two roles in the early subdivision of the lateral plate : specification and maintenance of the somatic mesoderm. We also show that the latter interactions are mediated by BMP molecules that are localized in the lateral ectoderm. Evolutionary aspects of the coelom formation are also considered.
|
