2002 Fiscal Year Final Research Report Summary
Differentiation of the glial cell environment, associated with the corticospinal tract development
| Project/Area Number |
11680730
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Saitama Medical School (2002)
Hokkaido University (1999-2001) |
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Principal Investigator |
NAGASHIMA Masabumi Saitama Medical School, Department of Anatomy, Professor, 医学部, 教授 (40241319)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Masahiko Hokkaido University Graduate School of Medicine, Department of Anatomy and Embryology, Professor, 大学院・医学研究科, 教授 (70210945)
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| Project Period (FY) |
1999 – 2002
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| Keywords | central pathways / morphogenesis / axon guidance / growth cone / target neuron / cell-to-cell interaction / epigenetic / developmental neurobiology |
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| Research Abstract |
The central nervous system networks are established by the axon outgrowth within the white matter fascicles and the subsequent synaptogenesis between the projection fiber and target neurons. The growth cones at the axon's tips show extensive motility and seem exploring behavior within the cellular environment. Such pathfinding dynamism can be represented by in vitro experiment using dissociated cell culture or explant slice culture system. Two major hypotheses concerning the possible mechanism underlying the wiring of neuronal circuitry have been investigated; namely "diffusible chemoattractant" which is secreted by the target neurons and "cell-contact-mediated guidance." Our standpoint is the later. Interaction between differentiating neuronal and glial cell populations is possibly mediated by receptor or transporter proteins incorporated within the surface membrane. Our result of this project showed drastic influence of the developmentally regulated cues in the spinal cord membranes to the cortical growth cone behaviors. Another result revealed active contribution of the Bergman glia, which were visualized by immunochemistry with glutamate transporter, to the morphogenesis of the Purkinje cell's dendrites. For further extension of understanding the problem of the neurite outgrowth, developmental neurobiology using in vivo experiments should be coupled with cell biology techniques, e.g. dynamics of the fluorescently-labeled cytoskeleton, monitored by the high-resolution time-lapse video microscopy.
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