2000 Fiscal Year Final Research Report Summary
PARTICIPATION OF TRANSLIN PROTEIN IN THE PROCESS OF DENDRITIC TRANSPORT OF BC1 RNA AND LOCAL PROTEIN SYNTHESIS IN THE VICINITY OF SYNAPSES.
| Project/Area Number |
11680765
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | NIHON UNIVERSITY |
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Principal Investigator |
ANZAI Kaijiro INSTITUTION, NIHON UNIVERSITY ; DEPARTMENT, COLLEGE OF PHARMACY ; TITLE OF POSITION, PROFESSOR., 薬学部, 教授 (30114359)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Shunsuke INSTITUTION, NIHON UNIVERSITY ; DEPARTMENT, COLLEGE OF PHARMACY ; TITLE OF POSITION, RESEARCH ASSOCIATE., 薬学部, 助手 (60256906)
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| Project Period (FY) |
1999 – 2000
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| Keywords | BC1RNA / Brain / Neuronal Dendrite / Pur α and Pur β Proteins / Microtubule-binding Protein / RNA Transport / Local Protein Synthesis / Neuronal Plasticity |
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| Research Abstract |
Neural BC1 RNA is distributed in neuronal dendrites as RNA-protein complexes (BC1 RNP) containing Translin. In this study, we demonstrated that the single stranded DNA- and RNA-binding protein, pur α and its isoform, pur β, which have been implicated in control of DNA replication and transcription, linked BC1 RNA to microtubules (MTs). The binding site was within the 5'-proximal region of BC1 RNA containing putative dendrite-targeting RNA motifs rich in G- and U-residues, suggesting that in the cytoplasm of neurons these nuclear factors are involved in the BC1 RNA transport along dendritic MTs. The pur proteins were not components of BC1 RNP, but appeared to associate with MTs in brain cells. Therefore, it is suggested that they may transiently interact with the RNP during transport. In this respect, the interaction of pur proteins with the BC1 RNA could be regulated by the Translin present within the RNP, since the binding mode of these two classes of proteins (pur proteins and Translin) to the dendrite-targeting RNA motifs was mutually exclusive. Since the motifs are well conserved in microtubule-associated protein 2 a/b mRNA as well, the pur proteins may also play a role (s) in the dendritic transport of a subset of mRNAs.
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[Publications] Li, Y., Koike, K., Ohashi, S., Funakoshi, T., Tadano, M., Kobayashi, S., and Anzai, K.: "Pur alpha protein implicated in dendritic RNA transport interacts with ribosomes in neuronal cytoplasm."Biol.Pharm.Bull.. (in press). (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ohashi, S., Kobayashi, S., Omori, A., Ohara, S., Omae, A., Muramatsu, T., Li, Y., and Anzai, K.: "The single-stranded DNA and RNA-binding proteins pur alpha and pur beta link BC1 RNA to microtubules through binding to the dendrite-targeting RNA motifs."J.Neurochemistry. 75. 1781-1790 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Kobayashi, S., Agui, K., Kamo, S., Li, Y., and Anzai, K.: "Neural BC1 RNA associates with pur alpha, a single-stranded DNA and RNA binding protein, which is involved in the transcription of the BC1 RNA gene."Biochem.Biophys.Res.Commun.. 277. 341-347 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Kobayashi, S., Kamo, S., Omae, A., Agui, K., Li, Y., and Anzai, K.: "Identification of a negative regulatory DNA element for neuronal BC1 RNA expression by RNA polymerase III."Biochim.Biophys.Acta.. 1493. 142-150 (2000)
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「研究成果報告書概要(欧文)」より
