2000 Fiscal Year Final Research Report Summary
Generation and analysis of NB-3 deficient mice
| Project/Area Number |
11680799
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
WATANABE Kazutada Tokyo Metropolitan Institute of Gerontology, Department of Cell Recognition, Head, 細胞認識部門, 室長 (70114717)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOYA Hiroko Tokyo Metropolitan Institute of Gerontology, Department of Cell Recognition, Research Asistant, 細胞認識部門, 実験助手 (00158841)
KOBAYASHI Satoru Tokyo Metropolitan Institute of Gerontology, Department of Cell Recognition, Researcher, 細胞認識部門, 研究員 (20100117)
TAKEDA Yasuo Tokyo Metropolitan Institute of Gerontology, Department of Cell Recognition, Siniror Researcher, 細胞認識部門, 研究員 (60245462)
IWAKURA Yoichiro University of Tokyo, Institute of Medical Science, Professor, 医科学研究所・ヒト疾患モデル動物センター, 教授 (10089120)
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| Project Period (FY) |
1999 – 2000
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| Keywords | contakutin subgroup / cell adhesion molecule / NB-3 deficient mice / behavior / motor coordination / Lac Z gene / expression |
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| Research Abstract |
Neural adhesion molecule NB-3 is one of the contactin/F3 subgroup in the immunoglobulin superfamily. We previously reported the generation and the characterization of NB-3 deficient mice that disrupted a part of NB-3 gene replacing with LacZ-PGK-neo cassette. By using heterozygotes the region specific expression of the NB-3 gene in the brain was determined and was found that NB-3 was highly expressed in Purkinje cell and internal granule cell layers in adult cerebellum. In this study, we examined the behavioral aspects of NB-3 deficient mice. Adult male mice of a mixed C57BL/6 and 129SvJ genetic background were used for this experiment. Gain of body weight up to 6 month old was not affected by NB-3 mutation. The exploratory locomotion and rearing activities of the mice were examined for 5 days in the open-field test. The mice were placed in 50 x 50 cm square field for 5 min a day. In the first trial the exploratory activities of NB-3 (-/-) mice were significantly lower than that of 8+/+) mice. Depending upon the subsequent trials, habituation of the activities was observed in wild type, but not in NB-3 (-/-) mice. The prehensile reflex and traction test evaluated that NB-3 deficient mice were significantly inferior ot the wild type in the muscle strength and tone. Besides the poor ability of NB-3 deficient mice to traverse a stationary horizontal rod resulted in dysfunction of psychomotor coordination. Thus, NB-3 is possibly implicated in the motor functions.
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[Publications] Ogawa, J., Lee S., itoh, K., Nagata, S., Machida, T., Takeda, Y.and Watanabe, K.: "Neural recognition molecule NB-2 of the contactin/F3 subgroup in rat : Specificity in neurite outgrowth-promoting activity and estricted expression in the brain regions."J.Neurosci. Res.. (in press).
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「研究成果報告書概要(欧文)」より
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[Publications] Fukamauchi, F., Aihara, O., Wang, Y.J., Akasaka, K., Takeda, Y., Sudo, K., Asano, M., Kawano, H., Watanabe, K.and Iwakura, Y.: "TAG-1 deficient mice have marked elevation of adenosine A1 receptors in the hippocampus."Biochem. Biophys. Res. Commun.. (in press).
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[Publications] Miyata, S., Takamatsu, H., Maekawa, S., Matsumoto, N., Watanabe, K., Kiyohara, T., Hattton, I.G.: "Plasticity of neurohypophysial terminals with increased hormonal release during dehydration ; ultrastructural and biochemical analysis."J.Comp. Neurol.. (in press).
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[Publications] Kamei, Y., Takeda, Y., Teramoto, K., Tsutsumi, O., Taketani, Y.and Watanabe, K.: "Human NB-2 of the contactin/F3 subgroup molecules : Chromosomal localization of the gene and deitinct expression pattern from other subgroup members."Genomics. 69. 113-119 (2000)
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[Publications] Lee, S., Takeda, Y., Kawano, H., Hosoya, H., Nomoto, M., Fujimoto, D., Takahashi, N.and Watanabe, K.: "Expression and regulation of a gene encoding neural recognition molecule NB-3 of the contactin/F3 subgroup in mouse brain."Gene. 245. 253-266 (2000)
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[Publications] Kasahara, K., Watanabe, K., Takeuchi, K., Kaneko, H., Oohira, A., Yamamoto, T.and Sanai, Y.: "Involvement of gangliosides in glycosylphosphatidylinositolanchored neuronal cell adhesion molecule TAG-1 signaling in lipid rafts."J.Biol. Chem.. 275. 34701-34709 (2000)
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「研究成果報告書概要(欧文)」より
