2001 Fiscal Year Final Research Report Summary
Speed congenics : application for functional analysis of multigenetic disease genes.
| Project/Area Number |
11680828
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | RIKEN (2000-2001)
Central Institute for Experimental Animals (1999) |
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Principal Investigator |
WAKANA Sigeharu RIKEN, Mouse Functional Genomics Research Group, Team Leader, マウス変異開発研究チーム, チームリーダー (90192434)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Multigenic disease / Congenic mouse / Microsatellite markers / NOD mouse / Speed congenics |
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| Research Abstract |
Congenic strains are developed by repeat backcross bleedings of a donor strain carrying a mutation or genetic region of interest to an inbred recipient strain. In the traditional backcrosses it takes 10-12 generations. The recent availability of dense maps of the mouse genome has allowed the development of microsatellite markers breeding strategies that aim to reduce the number of generations required to eliminate donor strain-derived alleles outside the genetic region of interest. Using marker-based selection for genetically optimal breeders at each generation recipient strain genome content can be reached several backckrosses. The time frame for derivation of "speed congenics" is essential method for the research of the gene function in the multigenetic disease models.
1.Several microsatellite genotyping panel sets have been developed that are polymorphic between NOD/Shi and MSM mice, or C57BL/6J, 129/SvJ. One set of markers for each strain pair has an intermarker distance of approximately 20cM. These sets were proven as useful tools for speed congenic development.
2.Type 1 diabetes is a multigenic autoimmune disease, the genetic basis for which is perhaps best characterized in the nonobese diabetic (NOD) mouse model. We developed these NOD congenic strains including susceptibility locus, Idd1, Idd3, Idd4, Idd4 and Idd9 using the speed congenic method.
3.The congenic strain of Idd1, linked to the major histocompatibility complex (MHC), were developed including the several genetic background.
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Research Products
(12 results)
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[Publications] Saito Y, Ochiai Y, Kodama Y, Tamura Y, Togashi T, Kosugi-Okano H, Miyazawa T, Wakabayashi Y, Hatakeyama K, Wakana S, Niwa O, Kominami: "Genetic loci controlling susceptibility to gamma-ray-induced thymic lymphoma"Oncogene. 37. 5243-5247 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Maruyama C, Tomisawa M, Wakana S, Yamazaki H, Kijima H, Suemizu H, Ohnishi Y, Urano K, Hioki K, Usui T, Nakamura M, Tsuchida T, Mitsumori K, Nomura T, Tamaoki N, Ueyama Y: "Overexpression of human H-ras transgene is responsible for tumors induced by chemical carcinogens in mice."Oncol Rep. 2. 233-237 (2001)
Description
「研究成果報告書概要(欧文)」より
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