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2001 Fiscal Year Final Research Report Summary

Joint studies on the nuclear-encoded photosystem genes.

Research Project

Project/Area Number 11694190
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionNagoya University

Principal Investigator

OBOKATA Junichi  Center for Gene Research, Nagoya University, Associate Professor, 遺伝子実験施設, 助教授 (50185667)

Co-Investigator(Kenkyū-buntansha) TANAKA Ryuichi  nstitute of Low Temperature, Hokkaido University, Research Associate, 低温科学研究所, 助手 (20311516)
TANAKA Ayumi  Institute of Low Temperature, Hokkaido University, Professor, 低温科学研究所, 教授 (10197402)
NAKAMURA Masayuki  Center for Gene Research, Nagoya University, Research Associate, 遺伝子実験施設, 助手 (60322145)
SUGIURA Masahiro  Graduate School of Natural Sciences, Nagoya City University Professor, システム自然科学研究科, 教授 (80027044)
Project Period (FY) 1999 – 2000
Keywordsphotosystems / core promoters / gene transfer / light strength / chlorophvll-a-oxygenase / light-harvesting antennae complex / RNAエディティング
Research Abstract

This research project was planned for promoting the international joint studies by the plant physiologists of Japan (five scientists mentioned above), US (Prof. Anastasios Melis, University of California, Berkeley), and Germany (Prof.Ralf Oelmueller, Friedrich-Schiller Universitaet, Jena) on the biogenesis of photosystem complexes in higher plants and algae. During the period supported by the grant, 21 scientists in total have visited the laboratories one another and stayed there for the total days of 148, and performed the cooperative studies. The outcomes of these research are 26 papers published in the journals and books, 17 presentations in the international meetings, and 29 presentations at domestic meetings, and two patent applications. The major scientific findings are as follows ;
1, We found that plant photosystem genes have unique promoter architecture and transcription initiation mechanisms for the first time ;
2, We showed that the biogenesis of photosystem complexes is subjected to positive feedback regulation by photosynthetic electron flow ;
3, The redox regulation of photosystem genes operates not only on mRNA abundance but also on poly-ribosome loading ;
4, We developed a set of special LED light sources that excite PSI and PSII alternatively ;
5, We revealed that the abundance of chlorophyll b is not so important for the biogenesis of light-harvesting antennas ;
6, CAO (chlorophyll-oxygenase) plays important roles in adjusting the antennae size to environmental light strength ;
7, We proposed a quite new hypothesis to explain the origin and evolution of chloroplats, based on the phylogenetic analyzes of CAO genes.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Miyamoto, T., T.Nakamura, I.Nagao, J.Obokata: "Quantitative analysis of the transiently expressed mRNA level in particle bombarded tobacco seedlings"Plant Molecular Biology Reporter. 18. 101-107 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maki, Y., A.Tanaka, A.Wada: "Stoichiometric analysis of barley plastid ribosomal proteins"Plant and Cell Physiology. 41. 289-299 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka, R., Y.Koshino, S.Sawa, S.Ishiguro, K.Okada, A.Tanaka: "Over expression of chlorophyllide a oxygenase (CAO) enlarges the antenna size of photosystem II in Arabidopsis"The Plant Journal. 26. 365-374 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, M., T.Tsunoda, J.Obokata: "Photosystem nuclear genes generally lack TATA boxes : a tobacco photosystem I gene responds to light through an initiator"The Plant Journal. 29. 1-10 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sheramati, I, M.Nakamura, Y.Yamamoto, T.Pfanschmidt, J.Obokata, R.Oelumuller: "Polyribosome loading of spinach mRNAs for photosystem I subunits is controlled by photosymthetic"The Plant Journal. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuo, M, J.Obokata: "Dual roles of photosynthetic electron transport in photosystem I biogenesis"Plant Physiology. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto,T., T.Nakamura, I.Nagao and J.Obokata: "Quantitative analysis of the transiently expressed mRNA level in particle bombarded tobacco seedlings"Plant Mol Biol Report. 18. 101-107 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maki, Y., Tanaka, A., Wada, A.: "Stoichiometric analysis of barley plastid ribosomal proteins"Plant Cell Physiol.. 41. 289-299 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka R., Koshino Y., Sawa S., Ishiguro S., Okada K and Tanaka A.: "Overexpression of chlorophyllide a oxygenase (CAO) enlarges the antenna size of photosystem II Arabidopsis thaliana"Plant J.. 26. 365-374 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura.M., T.Tsunoda and J.Obokata: "Photosynthesis nuclear genes generally lack TATA boxes : a tobacco Photosysteml gene responds to light through an initiator"Plant J.. 29. 1-10 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sherameti,I., M.Nakamura, Y.Y.Yamamoto, T. Pfannschmidt, J.Obokata and R. Oelmueller: "Polyribosome loading of spinach mRNAs for photosystem I subunits is controlled by the photosynthetic electron transport : A crucial cis-element in the spinach PsaD gene is located in the 5'-untranslated region."Plant J. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuo,M. and J.Obokata: "Dual roles of photosynthetic electron transport in Photosystem I biogenesis : Light induction of mRNAs and a chlomatic regulation at post-mRNA level"Plant Physiol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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