2000 Fiscal Year Final Research Report Summary
Molecular mechanisms regulating globin gene expression in fish
| Project/Area Number |
11694198
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Tokyo University of Fisheries |
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Principal Investigator |
AOKI Takashi Tokyo University of Fisheries, Faculty of Fisheries, Professor, 水産学部, 教授 (00051805)
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| Co-Investigator(Kenkyū-buntansha) |
HIRONO Ikuo Tokyo University of Fisheries, Faculty of Fisheries, Assistant Professor, 水産学部, 助手 (00270926)
MINAGAWA Sonomi Tokyo University of Fisheries, Faculty of Fisheries, JSPS
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| Project Period (FY) |
1999 – 2000
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| Keywords | globin gene / genome / molecular evolution / developmental stage specific expression |
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| Research Abstract |
We cloned eight carp α- and β-globin gene loci (No. 1 - 8). An RT-PCR analysis was performed to investigate the expression pattem of these three different types of globin genes during development. Among the three α-globin genes (1 α, 2 α, and 8 α), the 1 α gene was expressed in the early developmental stage but its expression soon decreased. The 8 α gene was dominantly expressed in embryos, but was diminished after hatching. At the juvenile stage, the 2 α gene showed the highest expression, while in the adults, the 1 α showed the highest expression. Among the three β-globin genes (1 β, 2 β, and 8 β), the expression pattem is similar to those of the α-globin genes. These results indicate that type 1 carp α- and β-globin genes are embryo and adult types, and the type 2 and 8 globin genes are larval types.
We amplified and sequenced the region within the 2nd exon of α- and β-globin genes of striped bass, Morone saxatilis. Three α-globin and 5 β-globin genes were identified by the DNA and predicted amino acid sequences. The phylogenetic tree of the amino acid sequences of the region within the 2nd exon of fish α- and β-globin showed that both the α-globins and the β-globins of striped bass could be divided into 2 groups. The phylogenetic tree also indicated that the divergence of fish globin genes were occured after diverged from other vertebrates. One group may be adult-type globins and the other may be embryo or non-adult stage globins. The phylogenetic tree also suggested that the globin genes have diverged in fishes independently from their divergence in land vertebrates.
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