2000 Fiscal Year Final Research Report Summary
Molecular biologic characterization of hatavirus pathogenicity
| Project/Area Number |
11694228
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Hokkaido University |
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Principal Investigator |
ARIKAWA Jiro Hokkaido University school of Medicine Professor, 医学部, 教授 (10142704)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMATSU Kumiko (吉松 組子) Hokkaido University school of Medicine Instructor, 医学部, 助手 (90220722)
KARIWA Hiroaki Hokkaido University Graduate School of Veterinary Medicine AssociateProfessor, 大学院・獣医学研究科, 助教授 (70224714)
TAKASHIMA Ikuo Hokkaido University Graduate School of Veterinary Medicine Professor, 大学院・獣医学研究科, 教授 (30002083)
MORIKAWA Shigeru National Institute of Infectious Disease Section of Virology, chief, ウイルス第一部・外来性ウイルス室, 室長(研究職) (00167686)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Hemorrhagic fever with renal syndrome / HFRS / hantavirus / zoonosis / virus infection / pathogenicity / genetic reassortant / infection enhancement |
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| Research Abstract |
1. Epidemiologic conditions and status of vaccine development in China were investigated. Information regarding to nucleotide sequence of Chinese isolates and diagnostic procedures for serodiagnosis were exchanged.
2. Technical information for genetic characterization of hantavirus was obtained from Slovak scientist.
3. Technical information for characterization of hantavirus receptor and genetic reassortant virus was obtained from US scientist.
4. Techniques for construction of artificial hantavirus was obtained from scientist of Wisconsin University School of Veterinary Medicine.
5. Information regarding to Nephropathia epidemica virus pathogenicity was obtained from Dr.Antti Vaheri of Helsinki University.
6. Information of epidemiologic and epizootiologic conditions in South East Asia was obtained from scientist in Thai.
7. Infection enhancement by the GalNac specific lectin, DBA and SBA was confirmed. Involvement of cellular factor on the virus cell membrane fusion was elucidated.
8. Pathogenicity to mice was related to growth rated at peripheral cite. The difference was considered to caused by the point mutation at enveloped protein.
9. Viral S and M genome was cloned and expressed in the mammalian cells. Several kinds of mini-genomes were constructed for the examination of role for transcription and translation. For the next step, relationship between the minigenome expression and the proteins expressed by S and M genomes will be required.
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