2001 Fiscal Year Final Research Report Summary
INTRINSIC ANTIMICROBIAL POLYPEPTIDE IN INFLAMMATROY BOWEL DISEASE
| Project/Area Number |
11694231
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | ASAHIKAWA MEDICAL COLLEGE |
|---|
Principal Investigator |
KOHGO Yutaka ASAHIKAWA MEDICAL COLLEGE, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (10133183)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ASHIDA Toshifumi ASAHIKAWA MEDICAL COLLEGE, SCHOOL OF MEDICINE, INSTRUCTOR, 医学部, 助手 (50261409)
AYABE Tokiyuoshi ASAHIKAWA MEDICAL COLLEGE, SCHOOL OF MEDICINE, INSTRUCTOR, 医学部, 助手 (90301019)
FUJIMOTO Yoshinori ASAHIKAWA MEDICAL COLLEGE, SCHOOL OF MEDICINE, P, INSTRUCTOR, 医学部, 助手 (90292127)
|
|---|
| Project Period (FY) |
1999 – 2001
|
| Keywords | INTERNATIONAL SCIENCE EXCHANGE / ANTIMICROBIAL PEPTIDE / a-DEFENSIN / PANETH CELL / INFLAMMATORY BOWEL DISEASE |
|---|
| Research Abstract |
In this study we analyzed the expression of antimicrobial peptide in IBD intestine and colon by biological and immunological technique. We have found that 1) As well as a mouse intestine reported before, isolated crypts from normal human intestine could exocrine the antimicrobial peptide by the LPS stimulation, ex vivo. 2) In situ hybridization and RT-PCR of isolated human crypts revealed that antiomicrobial activity was caused by human a-defensin (HD-5, HD-6), released from Paneth cells. 3) Isolated human crypts from IBD intestine also produce the antimicrobial peptide as well as that of normal, noninflammed intestine. 4) Paneth cells in UC colon (metaplastic Paneth cell) also release the antimicrobial activity. 5) Metaplastic Paneth cells in human stomach also have and release antimicrobial activity that kills effectively H. Pylori.
These results were submitted to publication.
|
