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2000 Fiscal Year Final Research Report Summary

Elucidation of the evolution and generation of human paralogous chromosome regions by genome analysis

Research Project

Project/Area Number 11694317
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionTokai University

Principal Investigator

INOKO Hidetoshi  Tokai University School of Medicine, Professor, 医学部, 教授 (10101932)

Co-Investigator(Kenkyū-buntansha) ANDO Asako  Tokai University School of Medicine, Assistant Professor, 医学部, 講師 (40101935)
Project Period (FY) 1999 – 2000
KeywordsHLA / genome duplication / evolution / homologous region / CD1 / glycosyltransferase / quadriplication / immune response
Research Abstract

In order to clarify the gene organization of the chromosome1q21-q22 region around the CD1 (non-classical MHC antigen) genes, and to understand of the evolution of the HLA region, we have determined the nucleotide sequence of a 1.1 Mb BAC and PAC contig segment joining CD1D to FCERIA.Twelve expressed genes were located in this region with the order of CD1D-CD1A-CD1C-CD1B-CD1E-SPTA1-MNDA-IFI-16-AIM2-BL1A-FY-FCERIA from centromere to telomere. In addition, 29 genes or possibly expressed gene sequences including 20 olfactory receptor (OR) genes were also found in this region. Dot matrix analysis using the entire HLA class I region sequence versus itself revealed numerous segmental duplications of a minimal building block, whereas no such trace of duplication units was observed in the CD1 region. Therefore, these findings suggest that the human CD1 region was established prior to the HLA class I region.
Some recent development of molecular biology has been interpreted as favoring the notion that the time of amphioxus emergence might be prior to two rounds of large scale genomic duplications around the time of vertebrate emergence. In order to clarify the gene organization of the region corresponding to the ancestral MHC region in amphioxus, we have determined the nucleotide sequence of in total 350, 250 bp genomic DNAs from 9 cosmid clones. Thirty-two genes or candidate gene sequences were found in these clones. It is noteworthy that the 14 of these 32 genes or candidate gene sequences showed high sequence homology to genes (RING3, h-Rev107-like, RXR, NEU-like, p44 protein, Notch, Selectin, etc.) Which were located in the human MHC region or the paralogous regions. Taken together, these findings suggest that amphioxus is a potential valuable model animal for understanding of the developmental process of the MHC region or the paralogous region.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Shiina T, et al.: "Molecular dynamics of MHC genesis unraveled by sequencing analysis of the 1,796,938 bp HLA class I region."Proc Natl Acad Sci USA. 96. 13282-13287 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] The MHC sequencing consortium (Aguado B, et al.): "Complete structure and gene map of a human major histocompatibility complex(MHC)."Nature. 401. 921-923 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiina T, et al.: "Genome sequencing analysis of the 1.8Mb entire human MHC class I region."Immunological Reviews. 167. 193-199 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dai KZ, et al.: "The SH2D2A gene encoding the T-cell-specific adapter protein(TSAd) is localized centromeric to the CD1 gene cluster on human chromosome 1."Immunogenetics. 51. 179-185 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keicho N, et al.: "Fine localization of a major disease-susceptibility locus for diffuse panbronchiolitis."Am J Hum Genet. 66. 501-507 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuzaka Y, et al.: "New polymorphic microsatellite markers in the human MHC class II region."Tissue Antigens. 56. 492-500 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiina et al.: "The beta 1,3-galactosyltransferase-4 (B3GALT4) gene is located in the centromeric segment of the human MHC class II region."Immunogenetics. 51. 75-78 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dai KZ, et al.: "The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd) is localized centromeric to the CD1 gene cluster on human chromosome 1."Immunogenetics. 51. 179-185 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keicho N, et al.: "Fine localization of a major disease-susceptibility locus for diffuse panbronchiolitis."Am J Hum Genet. 66. 501-507 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe Y, et al.: "Replication timing of the human X-inactivation center(XIC) region : correlation with chromosome bands."Gene. 252. 163-172 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ota M, et al.: "On the MICA deleted-MICB null, HLA-B4801 haplotype."Tissue Anitgens. 56. 268-271 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yabuki K, et al.: "Dev Ophthalmol"Kargar. 15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanaya S, et al.: "Major Histocompatibility Complex; Evolution, Structure, and Function"Springer. 15 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Komatsu-Wakui M, et al.: "Major Histocompatibility Complex; Evolution, Structure, and Function"Springer. 7 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiina T, et al.: "Molecular dynamics of MHC genesis unraveled by sequencing analysis of the 1,796,938 bp HLA class I region."Proc Natl Acad Sci USA. 96. 13282-13287 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] The MHC sequencing consortium (Aguado B, et al): "Complete structure and gene map of a human major histocompatibility complex (MHC)."Nature. 401. 921-923 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiina T, et al: "Genome sequencing analysis of the 1.8 Mb entire human MHC class I region."Immunological Reviews. 167. 193-199 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dai KZ, et al: "The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd) is localized centromeric to the CD1 gene cluster on human chromosome 1."Immunogenetics. 51. 179-185 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keicho N, et al: "Fine localization of a major disease-susceptibility locus for diffuse panbronchiolitis."Am J Hum Genet. 66. 501-507 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuzaka Y, et al: "New polymorphic microsatellite markers in the human MHC class II region."Tissue Antigens. 56. 492-500 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yabuki K, et al.: "Uveitis Update Immunogenetics"Dev.Ophthalmol.(ed.BenEzra) Basel, Kargar. 77-91 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanaya S, et al.: "Disribution of polypurine/polypyrimidine tract sequences in the human MHC region and their possible functions."Major Histocompatibility Complex (ed.Kasahar M), Springer, Tokyo. 131-145 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiina T et al.: "The beta 1, 3-galactosyltransferase-4 (B3GALT4) gene is located in the centromeric segment of the human MHC class II region."Immunogenetics. 51. 75-78 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dai KZ, et al.: "The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd) is localized centromeric to the CD1 gene cluster on human chromosome 1."Immunogenetics. 51. 179-185 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keicho N, et al.: "Fine localization of a major disease-susceptibility locus for diffuse panbronchiolitis."Am J Hum Genet. 66. 501-507 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe Y, et al.: "Replication timing of the human X-inactivation center (XIC) region : correlation with chromosome bands."Gene. 252. 163-172 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ota M, et al.: "On the MICA deleted-MICB null, HLA-B4801 haplotype."Tissue Anitgens. 56. 268-271 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Komatsu-Wakui M, et al.: "MIC-A polymorphism and a MIC-A-MIC-B null haplotype with Å'100kb deletion."Major Histocompatibility Complex (ed.Kasahar M), Springer, . Tokyo. 480-486 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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