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2000 Fiscal Year Final Research Report Summary

The Method of Microassay system of Sulfuric Glycosaminoglycans in saliva

Research Project

Project/Area Number 11694324
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionOsaka Dental University

Principal Investigator

OKAZAKI Joji  Osaka Dental University Lecturer, 歯学部, 講師 (80169094)

Co-Investigator(Kenkyū-buntansha) KAMADA Aiko  Osaka Dental University Research Assistant, 歯学部, 助手 (50140215)
Project Period (FY) 1999 – 2000
Keywordssaliva / Periodontal Disease / Diagnosis / Glycosaminoglicans / Condroitin palfate
Research Abstract

The measurement of sulphated glycosaminoglycans (S-GAGs) in gingival crevicular fluid (GCF) is important in the search of disease biomarkers. To analyze the amount of S-GAGs in GCF quickly and accurately, we have modified a microplate method previously used for urinary S-GAGs to estimate the content of S-GAGs in GCF without interference of other GCF components. GCF samples were collected from six maxillary anterior teeth and the diluted GCF samples were divided into two groups (20 μl each), with or without chondroitinase ABC treatment, mixed with a dimethylmethylene blue (DMB) dye solution (180 μl) to detect S-GAG.The absorbance was measured using microplate reader of 540 nm and the amount of S-GAG was calculated from calibration curves for standard S-GAGs where a linear relationship existed in the concentration range (0-3 μg/100 μl). Other GCF components such as glycoprotein and serum constituents did not influence the measurement of S-GAGs. Although trace amounts of S-GAGs were found in control with a healthy periodontium (0.05 μg/100 μl±0.04, mean±S.E., n=5), considerable amounts of S-GAGs were found in patients with advanced periodontal disease (0.42 ± 0.11, n=6, p<0.05). The accuracy of this simple and rapid method measuring S-GAGs in GCF makes it useful for screening periodontal diseases.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 岡崎定司,鎌田愛子,Graham Embeny: "歯周疾患ならびに顎関節疾患のバイオマーカーを用いたチェアーサイドでできるスクリーニング法"歯界展望-歯科医学と健康の創造. 増刊号. 321 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J OKAZAKI,A KAMADA,G EMBERY,RWADDINGTON,RMALL: "Measuring Glycosaminoglycans in TMJ Synotial Fluid Based on Dimethylmethylene Blue"J Dent Ples. 79・5. 1248 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.OKAZAKI, A KAMADA, G.EMBERY: "The Method of Microassar system of solfaric Glycosaminoglycans in saliva and TMJ"Dental Outlook, A special issue. 321. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.OKAZAKI, A.KAMADA, G.EMBERY, R.WADDINGTON, R.HALL: "Measuring Glycosaminoglycans in TMJ Synovial Fluid Based on Dimethylnethylone Blue."J.Dent Res. 179 (5). 1248 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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