2000 Fiscal Year Final Research Report Summary
The Method of Microassay system of Sulfuric Glycosaminoglycans in saliva
| Project/Area Number |
11694324
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Osaka Dental University |
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Principal Investigator |
OKAZAKI Joji Osaka Dental University Lecturer, 歯学部, 講師 (80169094)
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| Co-Investigator(Kenkyū-buntansha) |
KAMADA Aiko Osaka Dental University Research Assistant, 歯学部, 助手 (50140215)
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| Project Period (FY) |
1999 – 2000
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| Keywords | saliva / Periodontal Disease / Diagnosis / Glycosaminoglicans / Condroitin palfate |
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| Research Abstract |
The measurement of sulphated glycosaminoglycans (S-GAGs) in gingival crevicular fluid (GCF) is important in the search of disease biomarkers. To analyze the amount of S-GAGs in GCF quickly and accurately, we have modified a microplate method previously used for urinary S-GAGs to estimate the content of S-GAGs in GCF without interference of other GCF components. GCF samples were collected from six maxillary anterior teeth and the diluted GCF samples were divided into two groups (20 μl each), with or without chondroitinase ABC treatment, mixed with a dimethylmethylene blue (DMB) dye solution (180 μl) to detect S-GAG.The absorbance was measured using microplate reader of 540 nm and the amount of S-GAG was calculated from calibration curves for standard S-GAGs where a linear relationship existed in the concentration range (0-3 μg/100 μl). Other GCF components such as glycoprotein and serum constituents did not influence the measurement of S-GAGs. Although trace amounts of S-GAGs were found in control with a healthy periodontium (0.05 μg/100 μl±0.04, mean±S.E., n=5), considerable amounts of S-GAGs were found in patients with advanced periodontal disease (0.42 ± 0.11, n=6, p<0.05). The accuracy of this simple and rapid method measuring S-GAGs in GCF makes it useful for screening periodontal diseases.
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