1999 Fiscal Year Annual Research Report

双極性障害におけるミトコンドリア機能障害に関する分子生物学的研究

Research Project

Project/Area Number	11770536
Research Institution	The University of Tokyo
Principal Investigator	加藤忠史東京大学, 医学部・附属病院, 講師 (30214381)
Keywords	躁うつ病 / ミトコンドリア / カルシウム / 細胞内情報伝達系
Research Abstract	我々はこれまでに、双極性障害において脳エネルギー代償異常が見られること、ミトコンドリア遺伝子の5178Cおよび10398A多型が双極性障害の危険因子となることを見出してきた。一方、双極性障害の病態には細胞内カルシウム反応の亢進が関与すると考えられており、最近ミトコンドリアが細胞内カルシウム反応に関与することが分かってきた。本研究の目的は、双極性障害患者の培養リンパ芽球を用いて、細胞内カルシウム反応とミトコンドリア機能の関係について検討することである。これまでに双極性障害患者20名および対照群20名において、末梢血よりリンパ球を分離し、EBウイルスを用いてトランスフォーム後、培養を開始した。既に各々8名ずついおいて株化を確認した。これらの細胞に、蛍光細胞内カルシウム指示薬カルシウム指示薬Fura-2-AMを負担し、分化蛍光光度計を用いて、PAF(血小板活性化因子)、Thapsigargin、CCCPによる細胞内カルシウム反応を調べた。その結果、PAF刺激性カルシウム反応は双極性障害患者では低下傾向であり、Thapsigargin、CCCPによる細胞内カルシウム反応は上昇している傾向が見られた。今後例数を増やして検討していく予定である。

Research Products
(14 results)

All Other

All Publications (14 results)

[Publications] Kato T,Honda M 他: "A novel polymorphism in the promoter region of the tumor necrosis factor alpha gene;No association with narcolepsy."American Journal of Medical Genetics. 88. 301-304 (1999)
[Publications] Kato T,Murashita J 他: "Relationship of energy metabolism detected by ^<31>P-MRS in the human brain with mental fatigue.Neuropsvchobiology"Neuropsychobiology. 39. 214-218 (1999)
[Publications] Kato T,Honda M 他: "A search for a mutation in the tumor necrosis factor alpha(TNF-α)gene in narcolepsy."Psychiatry and Chinical Neurosciences. 53. 421-423 (1999)
[Publications] Kato T,Yamanaka G 他: "Efficacy of media in motivating patients with panic disorder to visit specialists."Psychiatry and Chinical Neurosciences. 53. 523-526 (1999)
[Publications] Kunugi H,Kato T 他: "No evidence for an association of polymorphism of the tryptophan hydroxylase gene with affective disorders or attempted suicide among Japanese patients."American Jourmal of Psychiatry. 156. 774-776 (1999)
[Publications] Murashita J,Kato T 他: "Age-dependent alteration of metabolic response to photic stimulation in the human brain measured by ^<31>PMR-spectroscopy."Brain Research. 818. 72-76 (1999)
[Publications] Kunugi H,Kato T 他: "Dinucleotide repeat polymorphism in the neurotrophin-3 gene and hippocampal volume in psychoses."Schizophrenia Research. 37. 271-273 (1999)
[Publications] Hamakawa H,Kato T 他: "Quantitative proton magnetic resonance spectroscopy in the bilateral frontal lobes of patients with bipolar disorder."Psychological Medicine. 29. 639-644 (1999)
[Publications] Kunugi H,Kato T 他: "A functional polymorphism in the promoter region of monoamine oxidase-A gene and mood disorders."Molecular Psychiatry. 4. 393-395 (1999)
[Publications] Kato T,Fujii K 他: "White matter hyperintensity detected by magnetic resonance imaging and lithium response in bipolar desorder;A preliminary observation."Psychiatry and Chinical Neurosciences. 54(in press). (2000)
[Publications] Kato T,Kunugi H 他: "Mitochondrial DNA polymorphisms in bipolar disorder."Journal of Affective Disorders. (in press). (2000)
[Publications] Sasaki T,Kato T 他: "Altered brain energy metabolism in lithium-resistant bipolar disorder detected by photic stimulated ^<31>P-MR spectroscopy."American Journal of Psychiatry. (in press). (2000)
[Publications] Kato T,Kato N 他: "Mitochondrial dysfunction in bipolar disorder."Bipolar Disorder. (in press). (2000)
[Publications] 加藤忠史: "双極性障害一躁うつ病の分子病理と治療戦略"医学書院. 201 (1999)

1999 Fiscal Year Annual Research Report

双極性障害におけるミトコンドリア機能障害に関する分子生物学的研究

Principal Investigator

加藤 忠史 東京大学, 医学部・附属病院, 講師 (30214381)

Research Products

[Publications] Kato T,Honda M 他: "A novel polymorphism in the promoter region of the tumor necrosis factor alpha gene;No association with narcolepsy."American Journal of Medical Genetics. 88. 301-304 (1999)

[Publications] Kato T,Murashita J 他: "Relationship of energy metabolism detected by ^<31>P-MRS in the human brain with mental fatigue.Neuropsvchobiology"Neuropsychobiology. 39. 214-218 (1999)

[Publications] Kato T,Honda M 他: "A search for a mutation in the tumor necrosis factor alpha(TNF-α)gene in narcolepsy."Psychiatry and Chinical Neurosciences. 53. 421-423 (1999)

[Publications] Kato T,Yamanaka G 他: "Efficacy of media in motivating patients with panic disorder to visit specialists."Psychiatry and Chinical Neurosciences. 53. 523-526 (1999)

[Publications] Kunugi H,Kato T 他: "No evidence for an association of polymorphism of the tryptophan hydroxylase gene with affective disorders or attempted suicide among Japanese patients."American Jourmal of Psychiatry. 156. 774-776 (1999)

[Publications] Murashita J,Kato T 他: "Age-dependent alteration of metabolic response to photic stimulation in the human brain measured by ^<31>PMR-spectroscopy."Brain Research. 818. 72-76 (1999)

[Publications] Kunugi H,Kato T 他: "Dinucleotide repeat polymorphism in the neurotrophin-3 gene and hippocampal volume in psychoses."Schizophrenia Research. 37. 271-273 (1999)

[Publications] Hamakawa H,Kato T 他: "Quantitative proton magnetic resonance spectroscopy in the bilateral frontal lobes of patients with bipolar disorder."Psychological Medicine. 29. 639-644 (1999)

[Publications] Kunugi H,Kato T 他: "A functional polymorphism in the promoter region of monoamine oxidase-A gene and mood disorders."Molecular Psychiatry. 4. 393-395 (1999)

[Publications] Kato T,Fujii K 他: "White matter hyperintensity detected by magnetic resonance imaging and lithium response in bipolar desorder;A preliminary observation."Psychiatry and Chinical Neurosciences. 54(in press). (2000)

[Publications] Kato T,Kunugi H 他: "Mitochondrial DNA polymorphisms in bipolar disorder."Journal of Affective Disorders. (in press). (2000)

[Publications] Sasaki T,Kato T 他: "Altered brain energy metabolism in lithium-resistant bipolar disorder detected by photic stimulated ^<31>P-MR spectroscopy."American Journal of Psychiatry. (in press). (2000)

[Publications] Kato T,Kato N 他: "Mitochondrial dysfunction in bipolar disorder."Bipolar Disorder. (in press). (2000)

[Publications] 加藤忠史: "双極性障害一躁うつ病の分子病理と治療戦略"医学書院. 201 (1999)

加藤忠史東京大学, 医学部・附属病院, 講師 (30214381)