2001 Fiscal Year Final Research Report Summary
Evaluation method development of endocrine disruptor effect and pollution analysis by its application
| Project/Area Number |
11793003
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
分離・精製・検出法
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| Research Institution | KUMAMOTO UNIVERSITY |
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Principal Investigator |
TODA Kei Kumamoto University, Faculty of Science, Associate professor, 理学部, 助教授 (90264275)
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| Co-Investigator(Kenkyū-buntansha) |
NAKONO Naoko Kumamoto Immuno Research Lab, Chief Researcher, 開発部, 主任研究員
NAKATA Haruhiko Kumamoto University, Graduate school of Science and Technology, Research Associate, 大学院・自然科学研究科, 助手 (60311875)
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| Project Period (FY) |
1999 – 2001
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| Keywords | endocrine disruptors / immunoassay / electrochemical sensor / polychlorinated biphenyls |
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| Research Abstract |
Some kinds of devices have been developed to determine specific chemicals electrochemically after immunoreactions. In the one of the devices, a SPR cell was used as a reaction field. Immobilization of antibody and immunoreactions were conducted with monitoring with the SPR signal and final product by enzyme reactions were detected electrochemically. This method had an advantage that the complicated procedure of sandwich immunoassay process could be monitored and could be successfully continued to the final electrochemical enzyme assay. The antigen could be measured by the SPR signal only when the concentration was high, and the electrochemical determination had 1000 time greater sensitivity than the SPR's. A reaction field was fabricated on a gold film by the process establish with the help of the SPR. Usually, the immobilization needs long time such as one night, when the antibody was conuected on a glass substrate with glutalaldehyde. On the contrary, the antibody could be immobilized on the gold film in a short time. When we used SPR instrument, the immobilization area was limited. But, by using the large immobilization area, the enzyme reaction could be done without the flow stop for the incubation. The other device consisted of a micro-capillary. With this capillary device, only 5 μL of sample was needed for the determination. The detection limit of IgG was very small, 2 amol. This device was expected to be used with inserting into a living body directly.
PCBs contained in living things and sediments were investigated by means of GC/MS. It was found that the PCB level at the bottom was higher than the mouth of the bay. PCBs levels in many samples of B. pectinirostris were measured. The concentration was related to the body length rather than to their age.
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