2001 Fiscal Year Final Research Report Summary
Development of SPR lipoprotein sensor for in situ clinical analysis
| Project/Area Number |
11793017
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
工業分析化学
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| Research Institution | Keio University |
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Principal Investigator |
SUZUKI Koji Keio University, Department of Applied Chemistry, Professor, 理工学部, 教授 (80154540)
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| Co-Investigator(Kenkyū-buntansha) |
KURIHARA Kazuyoshi Kanagawa Academy of Science and Technology, Researcher, 常勤研究員 (20270704)
SASAKI Shin-ichi Keio University, Department of Applied Chemistry, Instructor, 理工学部, 助手 (50317294)
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| Project Period (FY) |
1999 – 2001
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| Keywords | SPR sensor / optode / Chemical etching / Scanning near-field optical microscopy / SNOM / Miniaturization / Optical fiber probe / Surface plasmon resonance sensor |
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| Research Abstract |
A practical problem of SPR (Surface Plasmon Resonance) immuno sensor is the reduction of the sensitivity and specificity due to nonspecific interaction of proteins in human fluid (blood, tear). In this study we investigated the processes involved in the interaction of biomolecules with the surface of the sonsorchips and how to prevent nonspecific adsorption. Gold sonsor surfaces modified using self-assembled monolayers (SAMs) with various terminal groups (HS(CH_2)_<11>R) provide a wide range of different characteristics and therefore, are important regarding the design of immunoassays.
1) Sensorchip was prepared as a mixed SAM, using both ethylene glycol [R=(OCH_2CH_2)OH] terminated to thiols to reduce nonspecific protein binding and carboxylic acid [R=COOH] terminated thiols for binding of the antibody.
2) In order to increase the sensitivity, SG latex particles were immobilized on the surface of sensorchip.
3) The designed sensorchips were applied to the determination of the lipoprotein concentration in serum.
The sensorchip responded selectively to lipoprotein in serum, showing no significant nonspecific interaction. The properties of this chip were found to be sperior compared to the commercially available chip CM-5 (BIACORE). Further improvement of selectivity would make it possible to develop sensors for the clinical field.
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