| Project/Area Number |
11794014
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
生物資源科学
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
TAKEBA Go Kyoto Prefectural University, Human Environment, Professor, 人間環境学部, 教授 (10046500)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Hirofumi Kyoto Prefectural University, Human Environment, Research assistant, 人間環境学部, 助手 (50264750)
SHIURAISHI Toshimasa Kobe University, Agriculture, Research Assistant Fac., 農学部, 助手 (00304121)
SHIINA Takashi Kyoto Prefectural University, Human Environment, Assistant Professor, 人間環境学部, 助教授 (10206039)
MORI Mari Shiga Prefectural Agricultural Experiment Station, Main Researcher, 主任研究員
NAKAMURA Yasushi Kyoto Prefectural University, Research Assistant, 人間環境学部, 助手 (90285247)
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| Project Period (FY) |
1999 – 2001
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| Keywords | nitrate / biosensor / ammmoia / glutamine synthetase / eutrophication / de-nitrogenation / nitrate reductase / transformation |
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| Research Abstract |
A biosensor detecting nitrate in water was produced to investigate easily the nitrate concentration in river, lake or sea water. It converts nitrate to nitrite by nitrate reductase, and detects the nitrite by reacting with sulfanilamide and N-1-naphtyl ethylene. It detects 5ppm of nitrate in water. It will be used in various places to detect easily nitrate concentration.
In order to reduce the nitrogen in river, lake and sea water, we created the transgenic reed plants with over-expressed glutamine synthetase. The reed plants absorbed nitrate 15-to 20-times faster, and grew 3 times higher than control plants. It will be used efficiently to reduce the nitrate content in water.
Glutamine synthetase is a key enzyme to assimilate ammonia in roots of plants. It is expressed in plastids in roots and the level of expression is generally low in plants. We tried to over-express the glutamine synthetase in plastids by means of chloroplast transformation, and have got tobacco plants with 30-to 40-times higher content of glutamine synthetase. It will be used efficiently to reduce nitrogen in water.
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