| Co-Investigator(Kenkyū-buntansha) |
AYAKI Yoshikazu National College of Technology, Professor, 物質工学, 教授 (90032002)
MORIYAMA Masatsugu Tottori University, Faculty of Medicine, Associate. Professor, 医学部, 助教授 (90239707)
HASHIMOTO Eikich Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (20116239)
KASHIWAGI Kouji Daimatsu Co, Ltd. Sinner Researcher, 研究開発部, 技術員
NOGUCHI Makoto Industrial Research Institute of Tottori Prefecture, Sinner Researcher, 応用技術部, 研究員
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| Research Abstract |
In the marine food industry, fish skin is discarded as waste like bone and entrails, and these waste materials make-up more than a half of the whole body weight, desired for utilization in some other products. The skin of fish, which is composed of about 10% of fish waste, can be mechanically removed and readily recovered in food processing. The skin of a halibut Reinhararditus hippoglossoides, which is a main marine-food processing product in Tottori Prefecture Japan, contains 34% protein and related materials, 14% oil, and 52% water. The utilization of this fish skin is expected to contribute the development of local industry. Incidentally, liver diseases, such as hepatitis, liver cirrhosis and hepatoma were reported to show a tendency of rapid increase recently within Japanese people in the working generation of middle ages. It has also become an important subject to be urgently developed for therapeutic or ameliorative medication. At first in this project, the extraction and puriflcation process of collagen and extracellular matrix components were established using fish skin. As the application of fish collagen, we have succeeded the three dimensional culture of primary hepatocytes, and revealed that liver function was well maintained in this culture. Furthermore, we showed that proteoglycan crude extracts from the fish skin has significant inducibility of HGF in culture cell. Especially, of the separated glycosaminoglycans, dermatan sulfate which was the main component of glycosaminoglycans in the fish skin, showed the strongest activity in HGF induction. As the use of cattle collagen is restricted for the infection of the prion that is the cause of BSE and the Creutzfeldt-Jakob disease, the importance of fish collagen should be re-evaluated.
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