2000 Fiscal Year Annual Research Report

口蓋扁桃摘出手術は何歳になったら可能であるか?-ヒト口蓋扁桃リンパ球を移植したNOD-scidマウスでの免疫学的検討-

Research Project

Project/Area Number	11877294
Research Institution	Shimane Medical University
Principal Investigator	川内秀之島根医科大学, 医学部, 教授 (50161279)
Co-Investigator(Kenkyū-buntansha)	小笠原圭子島根医科大学, 医学部, 助手 (40304266) 片岡真吾島根医科大学, 医学部, 助手 (60152667) 加藤太二島根医科大学, 医学部, 助教授 (20185846)
Keywords	口蓋扁桃 / 粘膜免疫 / リンパ球 / ホーミング / NOD-scidマウス / Tリンパ球 / M蛋白 / IgA
Research Abstract	口蓋扁桃摘出手術やアデノイド切除術により得られたヒト扁桃リンパ球を採取し、フローサイトメトリーにより、リンパ球サブセットさらには抗原提示細胞について検討した。その結果、幼小児期から成人に至るまでのBおよびT細胞の割合、T細胞のサブセットであるヘルパーTリンパ球と抑制性Tリンパ球の割合についても詳細に検討し、細菌抗原の抗原提示に関わる抗原提示細胞の分布状況や、抗原認識や抗体産生の調節に関るTリンパ球サブセットの解析を行うことができた。さらに、ヒト扁桃リンパ球の共通粘膜免疫系組織へのホーミングならびに局所粘膜での機能分化について検討するため、ヒト扁桃由来の抗原特異的抗体産生B前駆細胞や抗体産生を調節するT細胞(1x10^8)をNOD-scidマウスに腹腔内投与し、経時的に脾臓に定着するかどうかについて検討した。その結果、ヒト扁桃リンパ球がTリンパ球を中心として、NOD-scidマウスに定着することが、成人より得られたヒト扁桃リンパ球を移入した際のフローサイトメトリーの結果より明らかにされた。さらに、定着したNOD-scidマウスにおいて、β溶連菌由来のM蛋白を粘膜アジュバントであるコレラトキシンと共に点鼻感作し、鼻粘膜局所での免疫応答ならびにヒト扁桃リンパ球のホーミングについて検討した。その結果、ヒト扁桃リンパ球を移入したマウス血清中には、M蛋白特異的なヒトIgGおよびIgA抗体が検出され、さらにM蛋白で点鼻感作した移入マウスでは、点鼻感作を行わなかった移入マウスに比べ、鼻洗浄液中のM蛋白特異的ヒトIgA抗体が有意に上昇していた。これらの結果は、ヒト口蓋扁桃リンパ球が共通粘膜免疫系の組織に帰趨し、分化・増殖することにより、粘膜局所で抗原特異的免疫応答を行っていることを示唆していると考えられる。

Research Products

(19 results)

All Other

All Publications (19 results)

[Publications] 柴宏巳: "ヒト鼻咽腔粘膜における抗原提示細胞の分布"耳鼻咽喉科免疫アレルギー. 16・1. 21-25 (1998)
[Publications] Tomoko Nagata: "Conjugation of ubiquitin-like polypeptide to intracellular acceptor proteins."Biochimica et Biophysica Acta. 1401. 319-328 (1998)
[Publications] 川内秀之: "ヒト口蓋扁桃リンパ球のマウスへの試み:NOD-scidマウスを用いた検討"口咽科. 10・2. 149-160 (1998)
[Publications] 川内秀之: "ヒト口蓋扁桃リンパ球のマウスへの試み"日本耳鼻咽喉科感染症研究会会誌. 16・1. 60-66 (1998)
[Publications] Hideyuki Kawachi: "The role of tonsils of as an Inductive site of Immune response in nasopharynx."Proceedings of the XIIth European Rhinologic Society and International Symposium on Infection and Allergy of the Nose, Vienna, Austria, July 28-August 1.1998. 83-87 (1998)
[Publications] Hideyuki Kawachi: "Mucosal immunity of nasopharynx."Proceedings of the XIIth European Rhinologic Society and International Symposium on Infection and Allergy of the Nose, Vienna, Austria, July 28-August 1.1998. 89-93 (1998)
[Publications] Chiaki Sano: "Therapeutic Effects of Benzoxazinorifamycin KRM- 1648 Secretory Leukocyte Protease Inhibitor or the Nonsteroidal Anti-Inflammatory Drug Diclofenac Sodium against Mycobacterium avium Complex Infection in Mice."Antimicrobial Agents And Chemotherapy. 43・2. 360-346 (1999)
[Publications] Chiaki Sano: "The modulating effects of proinflammatory cytokines, interferon-γ and tumor necrosis factor-α and immunoregulating cytokines. IL-10 and transforming growth factor-β, on antinicrobial activity of murine peritoneal macrophages against Mycobacterium avium-intracellulare"Clinical and Experimental Immunology. 115・3. 435-442 (1999)
[Publications] 川内秀之: "ヘルパーT細胞と調節性(抑制性)T細胞の今昔."耳鼻咽喉科免疫アレルギー. 17・1. 51-52 (1999)
[Publications] Tomoyuki Nishikori: "Apoptosis during inner ear development in human and mouse embryos : an analysis by computer-assisted three-dimensional reconstruction."Anat Embryol. 200. 19-26 (1999)
[Publications] Masashi Suzuki: "Efficacy of an antiallergic drug on otitis media with effusion in association with allergic rhinitis -An experimental study-."Annals of Otology, Rhinology & Laryngology. 108・6. 554-558 (1999)
[Publications] Masashi Suzuki: "Clinical efficacy of an antiallergic drug on otitis media with effusion in association with allergic rhinitis."Auris.Nasus.Larynx.. 26・2. 123-129 (1999)
[Publications] Hideyuki Kawauchi: "Mucosal immunity of nasopharynx -an experimental study in TCR-transgenic (OVA23-3) mice."Rhinology Supplement. 15. 56 (1999)
[Publications] Nobue Uchida: "Squamous cell carcinoma of the external auditory canal -Two cases treated with high dose rate^<192> Ir remote afterloading system (RALS)."Reprinted from Radiation Medicine. 17・6. 443-446 (1999)
[Publications] Hideyuki Kawauchi: "T-cell mucosal imminity in the nasopharynx."New Frontiers in Immunobiology. 35-41 (2000)
[Publications] 川内秀之: "上気道の生体防御機構と炎症性疾患の病態と治療."耳鼻咽喉科展望. 43・1. 10-22 (2000)
[Publications] Hisashi Ohga: "S-carboxymethy1Cysteine (S-CMC) has a suppressive effect on the Hydrogen Peroxide Production of Neutrophils."Recent Advances in Otitis Media. (in press).
[Publications] Ryoutaro Ishimitsu: "Overexpression of IL-15 In Vivo Enhances Tc1 Response, Which Inhibits Allergic Inflammation in a Murine Model of Asthma"The Journal of Immunology. 166. 1991-2001 (2001)
[Publications] Ryoutaro Ishimitsu: "Dichotomous effect of a traditional Japanese medicine, Bu-zhong-yi-qi-tang on allergic asthma in mice."Immunopharmacology. (in press).

2000 Fiscal Year Annual Research Report

口蓋扁桃摘出手術は何歳になったら可能であるか?-ヒト口蓋扁桃リンパ球を移植したNOD-scidマウスでの免疫学的検討-

Principal Investigator

川内 秀之 島根医科大学, 医学部, 教授 (50161279)

Research Products

[Publications] 柴宏巳: "ヒト鼻咽腔粘膜における抗原提示細胞の分布"耳鼻咽喉科免疫アレルギー. 16・1. 21-25 (1998)

[Publications] Tomoko Nagata: "Conjugation of ubiquitin-like polypeptide to intracellular acceptor proteins."Biochimica et Biophysica Acta. 1401. 319-328 (1998)

[Publications] 川内秀之: "ヒト口蓋扁桃リンパ球のマウスへの試み:NOD-scidマウスを用いた検討"口咽科. 10・2. 149-160 (1998)

[Publications] 川内秀之: "ヒト口蓋扁桃リンパ球のマウスへの試み"日本耳鼻咽喉科感染症研究会会誌. 16・1. 60-66 (1998)

[Publications] Hideyuki Kawachi: "The role of tonsils of as an Inductive site of Immune response in nasopharynx."Proceedings of the XIIth European Rhinologic Society and International Symposium on Infection and Allergy of the Nose, Vienna, Austria, July 28-August 1.1998. 83-87 (1998)

[Publications] Hideyuki Kawachi: "Mucosal immunity of nasopharynx."Proceedings of the XIIth European Rhinologic Society and International Symposium on Infection and Allergy of the Nose, Vienna, Austria, July 28-August 1.1998. 89-93 (1998)

[Publications] Chiaki Sano: "Therapeutic Effects of Benzoxazinorifamycin KRM- 1648 Secretory Leukocyte Protease Inhibitor or the Nonsteroidal Anti-Inflammatory Drug Diclofenac Sodium against Mycobacterium avium Complex Infection in Mice."Antimicrobial Agents And Chemotherapy. 43・2. 360-346 (1999)

[Publications] 川内秀之: "ヘルパーT細胞と調節性(抑制性)T細胞の今昔."耳鼻咽喉科免疫アレルギー. 17・1. 51-52 (1999)

[Publications] Tomoyuki Nishikori: "Apoptosis during inner ear development in human and mouse embryos : an analysis by computer-assisted three-dimensional reconstruction."Anat Embryol. 200. 19-26 (1999)

[Publications] Masashi Suzuki: "Efficacy of an antiallergic drug on otitis media with effusion in association with allergic rhinitis -An experimental study-."Annals of Otology, Rhinology & Laryngology. 108・6. 554-558 (1999)

[Publications] Masashi Suzuki: "Clinical efficacy of an antiallergic drug on otitis media with effusion in association with allergic rhinitis."Auris.Nasus.Larynx.. 26・2. 123-129 (1999)

[Publications] Hideyuki Kawauchi: "Mucosal immunity of nasopharynx -an experimental study in TCR-transgenic (OVA23-3) mice."Rhinology Supplement. 15. 56 (1999)

[Publications] Nobue Uchida: "Squamous cell carcinoma of the external auditory canal -Two cases treated with high dose rate^<192> Ir remote afterloading system (RALS)."Reprinted from Radiation Medicine. 17・6. 443-446 (1999)

[Publications] Hideyuki Kawauchi: "T-cell mucosal imminity in the nasopharynx."New Frontiers in Immunobiology. 35-41 (2000)

[Publications] 川内秀之: "上気道の生体防御機構と炎症性疾患の病態と治療."耳鼻咽喉科展望. 43・1. 10-22 (2000)

[Publications] Hisashi Ohga: "S-carboxymethy1Cysteine (S-CMC) has a suppressive effect on the Hydrogen Peroxide Production of Neutrophils."Recent Advances in Otitis Media. (in press).

[Publications] Ryoutaro Ishimitsu: "Overexpression of IL-15 In Vivo Enhances Tc1 Response, Which Inhibits Allergic Inflammation in a Murine Model of Asthma"The Journal of Immunology. 166. 1991-2001 (2001)

[Publications] Ryoutaro Ishimitsu: "Dichotomous effect of a traditional Japanese medicine, Bu-zhong-yi-qi-tang on allergic asthma in mice."Immunopharmacology. (in press).

川内秀之島根医科大学, 医学部, 教授 (50161279)