1999 Fiscal Year Annual Research Report

体液からの年齢推定に関する法医学的研究

Research Project

Project/Area Number	11897010
Research Institution	Gunma University
Principal Investigator	岸紘一郎群馬大学, 医学部, 教授 (30169841)
Co-Investigator(Kenkyū-buntansha)	安田年博群馬大学, 助教授 (80175645) 滝澤久夫富山医科薬科大学, 医学部, 教授 (90171579) 三澤章吾筑波大学, 社会医学系, 教授 (50086534) 飯田礼子福井医科大学, 医学部, 助手 (40139788) 竹下治男群馬大学, 講師 (90292599)
Keywords	個人識別 / 年齢推定 / 体液 / 分子生物学 / 尿
Research Abstract	法医学において個人識別は極めて重要な研究分野の一つであるが、現在のところ血液などの体液から年齢推定可能な年齢依存性変化を示すマーカーはなく、これらマーカの発見・法医学実務への導入が図られなければならない。そこで、本研究では血液や体液から年齢推定に活用しうるマーカーを開発すべく予備実験、文献調査および討議を重ね、本課題を将来「重点領域研究」の研究課題とする予備調査を目的とした。 1.従前の研究において17歳以下の若年者尿中には存在するが、それ以上になると検出できなくなる年齢依存性タンパク質(Ugl-Y)を見出した。今回、若年者尿から精製したUgl-Yに関するタンパク質化学的解析から、Ugl-Yの全アミノ酸配列の95%以上を決定した。さらに、その構造はfibronectinの一領域にほぼ一致することが明らかとなった。2.年齢推定に利用しうる年齢依存性生体分子を検索するため分子生物学的アプローチとして遺伝子発現パターンの年齢依存性変動を精査した。遺伝子発現の変動をモニターする有効な方法であるfluorescence differential display(FDD)-PCR法を利用して、異なった成長段階におけるマウス腎臓の遺伝子発現を解析した。その結果、7種類の遺伝子で有意な年齢依存的発現の認められることを見出した。そのなかには老年期のみに発現されるものなど様々な年齢依存的発現パターンが観察された。本研究結果はFDD-PCR法が年齢依存的発現を示す遺伝子の検索には有効な手段であることを証明するものであった。今後は、本法によって見出された年齢依存的発現を示す遺伝子について、その産物が法医学的試料から検出しうるものか否かを検討して年齢推定マーカーとして活用する予定である。以上の研究成果、文献調査などから、年齢依存性マーカーの発見・実務への応用に関する基本的コンセンサスが得られ、それに沿って研究を継続する予定である。

Research Products

(33 results)

All Other

All Publications (33 results)

[Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains"The Journal of Forensic Sciences. 44(1). 175-178 (1999)
[Publications] Toshihiro Yasuda: "Structural requirement of human deoxyribonuclease II for the development of the active enzyme form,revealed by site-directed mutagenesis"Biochemocal and Biophysical Research Communications. 256(3). 591-594 (1999)
[Publications] Osamu Hosomi: "Xenopus laevis pancreatic Dnase I:purification and characterization"Experimental and Clinical Immunogenetics. 19(2). 117-123 (1999)
[Publications] Toshihiro Yasuda: "A new allele,DNASE1^*6,of human deoxyribouuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochemical and Biophysical Research Communications. 260(1). 280-283 (1999)
[Publications] Haruo Takeshita: "Simultaneous typing of the STR loci,vWF and HumTPO,using discontinuous polyacrylamide gel electrophoresis"Legal Medicine. 1(3). 233-237 (1999)
[Publications] Yoshimitsu Nakashima: "Molecular,biochemical and immunological studies of hen pancreatic deoxyribonuclease I"International Journal of Biochemistry and Cell Biology. 31(11). 1313-1324 (1999)
[Publications] Einosuke Tanaka: "Clinically significant pharmacokinetic drug interactions with psychoactive drugs"Journal of Clinical Pharmacy and Therapeutics. 24. 7-16 (1999)
[Publications] Katsuya Honda: "Identification of a dismembered body by DNA analysis"International Medical Journal. 61(2). 151-154 (1999)
[Publications] Yoshihiko Kominato: "Expression of human histo-blood group ABO genes is dependent upon DNA methylation of the promoter region"Journal of Biological Chemistry. 274. 37240-37250 (1999)
[Publications] Reiko Iida: "Age-related changes of gene expression in mouse kidney:fluorescence differential display-PCR analysis"Mechanisms of Ageing and Development. 113(2). 133-142 (2000)
[Publications] Yoshihiko Kominat: "Acute cerebellar hemorrhage in a patinet with Klinefelter syndrome"The Journal of Forensic Sciences. (in press).
[Publications] Ichiro Shimada: "DNA polymorphisms in the 5'-flanking sequence of human ABO blood group genes and their association with the alleles for the common ABO phenotypes"Legal Medicine. 1(4). 217-225 (1999)
[Publications] Akira Kido: "AmpliType PM+DQA1 typing on aged seminal stains"Acta Criminologiae et Medicinae Legalis Japonica. 65(2). 66-69 (1999)
[Publications] I.Kondo: "Missense mutations in phosphomannomutase 2 gene in two Japanese families with carbohyrate-deficient glycoprotein syndrome type 1"Clinical Genetics. 55(1). 50-54 (1999)
[Publications] H.Zang: "Novel TSC2 mutation in patient with pulmonary tuberous sclerosis:lack of loss of heterozygosity in a lung cyst"American Journal of Medical Genetics. 82(5). 368-370 (1999)
[Publications] M.Nakagawa: "A new single-nucleotide polymorphism in the seventh componenet of complement (C7) gene"Journal of Human Genetics. 44(4). 272-273 (1999)
[Publications] K.Mizugishi: "Missenes mutations in the phosphomannomutase 2 gene of two Japanese siblings with carbohydrate-deficient glycoprotein syndrom type 1"Braon and Development. 21(3). 223-228 (1999)
[Publications] K.Yoshikawa: "Determination of carbohydrate-deticient transterrin separated by lectin affinity chromatography for detecting chronic alcohol abuse"FEBS Letters. 458(2). 112-116 (1999)
[Publications] Tamiko Nakajima: "Rapid purification of human Dnase I using mouse monoclonal anti-Dnase I antibodies and characterization of the antibodies"Experimental and Clinical Immunogenetics. (in press).
[Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (Dnase II)"FEBS Letters. 467(1/2). 231-234 (2000)
[Publications] Osamu Hosomi: "Molecular cloning of cDNA encoding Xenopus Laevis deoxyribonuclease I"DNA Sequence. (in press).
[Publications] 藤川克行: "日本人のトランスフェリン多型に認められる地理的勾配(埼玉県および青森県在住者を中心として)"血液事業. 21(4). 279-287 (1999)
[Publications] 安田年博: "ヒトdeoxyribonuclease II(Dnase II)に関する最近の研究"北関東医学. 49(1). 39-41 (1999)
[Publications] 岸紘一郎: "Dnase I"日本臨床. 57(増刊). 486-489 (1999)
[Publications] 竹下治男: "不連続系ポリアクリルアミドゲル電気泳動を利用した2種類のSTR座位(vWFおよびHumTPO)の同時検出"DNA多型. 7. 88-90 (1999)
[Publications] 渡邊剛太郎: "Heteroduplex analysis によるSTR多型解析"DNA多型. 7. 124-128 (1999)
[Publications] 三次綾: "西日本と韓国におけるalchoholdehydrogenase 2(ADH2)およびaldehyde dehydrogenase 2(ALDH2)の遺伝的多型について"DNA多型. 7. 158-160 (1999)
[Publications] 梅津和夫: "凝固因子FXIIIBとSTR多型と蛋白多型との関連について"DNA多型. 7. 241-243 (1999)
[Publications] 村脇義和: "ALDH2遺伝子型の飲酒習慣およびアルコール性肝障害への影響:健康診断受診者での検討"アルコールと医学生物学. 19. 74-77 (1999)
[Publications] 三澤章吾: "自動車事故における人体損傷の形成機序"法医学の実際と研究. 42. 1-16 (1999)
[Publications] 三澤章吾: "ミクロからマクロへ―個から集団へ"學鐙. 96(3). 10-13 (1999)
[Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane:a case study and animal experiments"International Journal of Legal Medicine. (in press).
[Publications] Haruo Takeshita: "Mammalian deoxyribonucleases I are classified into three types:pancreas,parotid and pancreas-parotid(mixed) types,based on differences of their tissue concentrations"Biochemical and Biophysical Reseach Communications. (in press).

1999 Fiscal Year Annual Research Report

体液からの年齢推定に関する法医学的研究

Principal Investigator

岸 紘一郎 群馬大学, 医学部, 教授 (30169841)

Research Products

[Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains"The Journal of Forensic Sciences. 44(1). 175-178 (1999)

[Publications] Toshihiro Yasuda: "Structural requirement of human deoxyribonuclease II for the development of the active enzyme form,revealed by site-directed mutagenesis"Biochemocal and Biophysical Research Communications. 256(3). 591-594 (1999)

[Publications] Osamu Hosomi: "Xenopus laevis pancreatic Dnase I:purification and characterization"Experimental and Clinical Immunogenetics. 19(2). 117-123 (1999)

[Publications] Toshihiro Yasuda: "A new allele,DNASE1^*6,of human deoxyribouuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochemical and Biophysical Research Communications. 260(1). 280-283 (1999)

[Publications] Haruo Takeshita: "Simultaneous typing of the STR loci,vWF and HumTPO,using discontinuous polyacrylamide gel electrophoresis"Legal Medicine. 1(3). 233-237 (1999)

[Publications] Yoshimitsu Nakashima: "Molecular,biochemical and immunological studies of hen pancreatic deoxyribonuclease I"International Journal of Biochemistry and Cell Biology. 31(11). 1313-1324 (1999)

[Publications] Einosuke Tanaka: "Clinically significant pharmacokinetic drug interactions with psychoactive drugs"Journal of Clinical Pharmacy and Therapeutics. 24. 7-16 (1999)

[Publications] Katsuya Honda: "Identification of a dismembered body by DNA analysis"International Medical Journal. 61(2). 151-154 (1999)

[Publications] Yoshihiko Kominato: "Expression of human histo-blood group ABO genes is dependent upon DNA methylation of the promoter region"Journal of Biological Chemistry. 274. 37240-37250 (1999)

[Publications] Reiko Iida: "Age-related changes of gene expression in mouse kidney:fluorescence differential display-PCR analysis"Mechanisms of Ageing and Development. 113(2). 133-142 (2000)

[Publications] Yoshihiko Kominat: "Acute cerebellar hemorrhage in a patinet with Klinefelter syndrome"The Journal of Forensic Sciences. (in press).

[Publications] Ichiro Shimada: "DNA polymorphisms in the 5'-flanking sequence of human ABO blood group genes and their association with the alleles for the common ABO phenotypes"Legal Medicine. 1(4). 217-225 (1999)

[Publications] Akira Kido: "AmpliType PM+DQA1 typing on aged seminal stains"Acta Criminologiae et Medicinae Legalis Japonica. 65(2). 66-69 (1999)

[Publications] I.Kondo: "Missense mutations in phosphomannomutase 2 gene in two Japanese families with carbohyrate-deficient glycoprotein syndrome type 1"Clinical Genetics. 55(1). 50-54 (1999)

[Publications] H.Zang: "Novel TSC2 mutation in patient with pulmonary tuberous sclerosis:lack of loss of heterozygosity in a lung cyst"American Journal of Medical Genetics. 82(5). 368-370 (1999)

[Publications] M.Nakagawa: "A new single-nucleotide polymorphism in the seventh componenet of complement (C7) gene"Journal of Human Genetics. 44(4). 272-273 (1999)

[Publications] K.Mizugishi: "Missenes mutations in the phosphomannomutase 2 gene of two Japanese siblings with carbohydrate-deficient glycoprotein syndrom type 1"Braon and Development. 21(3). 223-228 (1999)

[Publications] K.Yoshikawa: "Determination of carbohydrate-deticient transterrin separated by lectin affinity chromatography for detecting chronic alcohol abuse"FEBS Letters. 458(2). 112-116 (1999)

[Publications] Tamiko Nakajima: "Rapid purification of human Dnase I using mouse monoclonal anti-Dnase I antibodies and characterization of the antibodies"Experimental and Clinical Immunogenetics. (in press).

[Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (Dnase II)"FEBS Letters. 467(1/2). 231-234 (2000)

[Publications] Osamu Hosomi: "Molecular cloning of cDNA encoding Xenopus Laevis deoxyribonuclease I"DNA Sequence. (in press).

[Publications] 藤川克行: "日本人のトランスフェリン多型に認められる地理的勾配(埼玉県および青森県在住者を中心として)"血液事業. 21(4). 279-287 (1999)

[Publications] 安田年博: "ヒトdeoxyribonuclease II(Dnase II)に関する最近の研究"北関東医学. 49(1). 39-41 (1999)

[Publications] 岸紘一郎: "Dnase I"日本臨床. 57(増刊). 486-489 (1999)

[Publications] 竹下治男: "不連続系ポリアクリルアミドゲル電気泳動を利用した2種類のSTR座位(vWFおよびHumTPO)の同時検出"DNA多型. 7. 88-90 (1999)

[Publications] 渡邊剛太郎: "Heteroduplex analysis によるSTR多型解析"DNA多型. 7. 124-128 (1999)

[Publications] 三次綾: "西日本と韓国におけるalchoholdehydrogenase 2(ADH2)およびaldehyde dehydrogenase 2(ALDH2)の遺伝的多型について"DNA多型. 7. 158-160 (1999)

[Publications] 梅津和夫: "凝固因子FXIIIBとSTR多型と蛋白多型との関連について"DNA多型. 7. 241-243 (1999)

[Publications] 村脇義和: "ALDH2遺伝子型の飲酒習慣およびアルコール性肝障害への影響:健康診断受診者での検討"アルコールと医学生物学. 19. 74-77 (1999)

[Publications] 三澤章吾: "自動車事故における人体損傷の形成機序"法医学の実際と研究. 42. 1-16 (1999)

[Publications] 三澤章吾: "ミクロからマクロへ―個から集団へ"學鐙. 96(3). 10-13 (1999)

[Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane:a case study and animal experiments"International Journal of Legal Medicine. (in press).

[Publications] Haruo Takeshita: "Mammalian deoxyribonucleases I are classified into three types:pancreas,parotid and pancreas-parotid(mixed) types,based on differences of their tissue concentrations"Biochemical and Biophysical Reseach Communications. (in press).

岸紘一郎群馬大学, 医学部, 教授 (30169841)