2005 Fiscal Year Final Research Report Summary
Molecular mechanism of viral pathogenicity and of viral mutplisation.
| Project/Area Number |
12052201
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Hokkaido University |
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Principal Investigator |
UYEDA Ichiro Hokkaido University, Graduate School of Agriculture, Prof., 大学院農学研究科, 教授 (10113523)
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| Co-Investigator(Kenkyū-buntansha) |
MASUTA Chikara Hokkaido University, Graduate School of Agriculture, Prof., 大学院農学研究科, 教授 (60281854)
ISHIKAWA Masayuki National Institute of Agrobiobgical Sciences, Group Leader, 生理機能研究グループ, チーム長 (70192482)
MISE Kazuyuki Kyoto Universky, Graduate School of Agriculture, Assoc.Prof., 大学院農学研究科, 助教授 (90209776)
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| Project Period (FY) |
2000 – 2005
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| Keywords | Plant virus / Pathogenicity / Multiplication / Host-virus Interactions |
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| Research Abstract |
HC-Pro gene of clover yellow vein virus was proven to play a crucial role in inducing necrosis in legume plants. Using an infectious cDNA to the virus, many mutants were generated and inoculated to plants. And several point mutations were identified controlling necrosis induction.
We analyzed the functions of the 2b proteins of cucumoviruses in the viral symptom induction. The 2b protein of Tomato aspermy virus was found to be involved in the systemic movement in tobacco. The 2b protein of cucumber mosaic virus soybean strain induces seed coat mottling of soybean by suppressing RNA silencing of the chalcone synthase genes.
The Arabidopsis CUM1 and CUM2 genes were cloned that are involved in cucumber mosaic virus multiplication. An in vitro assay system for the Tm-1 activity in tomato that inhibits the tomato mosaic virus RNA replication was also established.
To analyze movement and adaptation mechanisms of bromoviruses, functional domains of the movement protein (MP) and coat protein of Brome mosaic virus (BMV) were mapped and a BMV MP-interacting host plant protein was identified and shown to be involved in cell-to-cell movement of BMV.
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[Journal Article] Patterning of virus-infected soybean seed coat is associated with suppression of endogenous silencing of chalcone synthase genes.2004
Author(s)
Senda, M., Masuta, C., Ohnishi, S., Goto, K., Kasai, A., Sano, T, Hong, J.-S., MacFarlane, S.
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Journal Title
The Plant Cell 16
Pages: 807-818
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and characterization of the SSB1 locus involved in symptom development by Spring beauty latent virus infection in Arabidopsis thaliana.2004
Author(s)
Fujisaki, K., Hagihara, F., Azukawa, Y., Kaido, M., Okuno, T., Mise, K.
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Journal Title
Molecular Plant-Microbe Interactions 17
Pages: 967-975
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Development of clover yellow vein virus as an efficient, stable gene expression system for legume species.2000
Author(s)
Masuta, C., Yamana, T., Tacahashi, Y., Uyeda, I., Sato, M., Ueda, S., Matsumura, T.
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Journal Title
The Plant Journal 23
Pages: 539-546
Description
「研究成果報告書概要(欧文)」より
