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2005 Fiscal Year Final Research Report Summary

Elcilor reception and molecLiar mechanisms and fundion of oxidative bust

Research Project

Project/Area Number 12052212
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

DOKE Noriytiti  NAGOYA UNIVERSITY, Graduate School of Bioagricultral Sciences, Prof., 大学院生命農学研究科, 教授 (80023472)

Co-Investigator(Kenkyū-buntansha) SHIBUYA Naoto  Meiji University, Factity of Agrbulture, Prof., 農学部, 教授 (70350270)
Project Period (FY) 2000 – 2005
KeywordsElicitor / receptor of plucan elicitor / oxidative burst / MAP kinase cascade / PVS3 promoter / rboh / Disease-tolerant potato / gene silencing
Research Abstract

Chitin-oligosaccharidesβ-glucan and lipopolysacharides elicitors, which had potential to induce oxidative burst and phytoalexin production in suspension cultured cells of rice plant, were isolated and characterized. Receptors of the elicitors were isolated and characterized. Interaction between elicitor and receptor was followed by activation of phospholypase under regulation by protein kinases in a close relation to activation of active oxygen generation.. Two sets of NADPH oxidase genes were cloned from potato and Nicotiana benthamiana, named StrbohA and StrbohB and NbrbohA and NbrbohB, respectively.
In potato tissues infected with Phyiophthora infestans, Ca^<2+> signaling was immediately stimulated, following activation of oxidative burst system consisted of Strboh A through Ca^<2+> dependent protein kinase in the case of incompatible interaction. A MAP kinase cascade involving StMEK 1 and StMAPI was stimulated in both compatible and incompatible interaction, followed by activation of gene expression of StrbohB for secondary oxidative bust and enzymes for synthesis of sesuquiterpenoid phytoalexin, HMG, PVS etc.. However, activation of these enzymes was stimulated in relation to the first oxidative bust characteristic of incompatible interaction. StMEK1^<DD>, a constantly active form of StMEK1, was found to stimulate the oxidative burst depending on StrbohB, As RSV3, gene of vestipiradien syntase was sensitively response to infection with compatible race, StMEK1^<DD> conjugated with the promotor of PVS3 was subjected to toransformation of potato cultivars. The transformed potato plants expressed tolerance to P infestans, Ahernaria solani, or Erwinia cartovola with an activation of MAP kinase cascade, expression of StrbohB, response of oxidative burst and hypersensitive resistant reaction.

  • Research Products

    (56 results)

All 2005 2004 2003 2002 2001 2000

All Journal Article (54 results) Book (2 results)

  • [Journal Article] Elicitor-induced activation of phospholipases plays an important role for the induction of defense responses in suspension-cultured rice cells2005

    • Author(s)
      T.Yamaguchi
    • Journal Title

      Plant Cell Physiol 46

      Pages: 579-587

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The superoxide-generating NADPH oxidase in plants : its molecular basis and role in induction of local and systemic induced resistance against pathogens2005

    • Author(s)
      N.Doke, et al.
    • Journal Title

      Genomic and Genetic Analysis of Plant Parasitism and Defense.(S.Tsuyumu, H.E.Leach, T.Shiraishi, and T.Wolpert.. (eds.))(APS Press, St.Paul MN.)

      Pages: 195-206

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Elicitor-induced activation of phospholipases plays an important role for the induction of defense responses in suspension- cultured rice cells.2005

    • Author(s)
      T.Yamaguchi et al.
    • Journal Title

      Plant Cell Physiol. 46

      Pages: 579-546.579-587

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The oxidative burst in infected plants : its mechanism, function and application for disease tolerance.2005

    • Author(s)
      N.Doke et al.
    • Journal Title

      In Pursuit of the Essence of Plant Pathogenesis.(S.Takamatsu, I.Kobayashi, H.Yoshioka, K.Toyota (eds))(Mie University, Mie.)

      Pages: 28-34

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Involvement of nitric oxide generation in hypersensitive cell death induced by elicitin in tobacco cell suspension culture.2004

    • Author(s)
      Yamamoto, A
    • Journal Title

      J.Gen.Plant Pathol. 70

      Pages: 85-92

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular cloning and characterization of a cDNA encoding ent-cassa-12, 15-diene symthase, a putative diterpenoid phytoalexin biosynthetic enzyme. from susp@ension Cultured reice cells treated with a chitin elicitor2004

    • Author(s)
      E.-M.Cho
    • Journal Title

      Plant J. 37

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Two rice GRAS family genesresponsive to N-acctylchitooligosaccharide elicitor are induced by phytoactivegibberellins : Evidence for cross talk between elicitor-and gibberellin-signaling in rice cells.2004

    • Author(s)
      R.B.Day
    • Journal Title

      Plant Mol.Biol 54

      Pages: 261-272

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization and cDNA cloning of monomeric lectins that correspond to the B-chain of a type 2 ribosome-inactivating protein from the bark of Japanese elderberry (Sambucus sieboldiana).2004

    • Author(s)
      M.A.Rojo
    • Journal Title

      J.Biochem 135

      Pages: 509-516

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation of Phospholipase D Induced by Hydrogen Peroxide in Suspension cultured Rice Cells2004

    • Author(s)
      T.Yamaguchi
    • Journal Title

      Plant Cell Physiol 45

      Pages: 1261-1270

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice2004

    • Author(s)
      T.Nemoto
    • Journal Title

      FEBS Lett 571

      Pages: 182-186

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] RERJ1, a jasmonic acid-responsive gene from rice endodes a basic helix-loop-helix protein. Biochem. Biophys.2004

    • Author(s)
      K.Kiribuchi
    • Journal Title

      Biochem.Biophys.Res.Comm 325

      Pages: 857-863

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Involvement of nitric oxide generation in hypersensitive cell death induced by elicitin in tobacco cell suspension culture.2004

    • Author(s)
      A.Yamamoto et al.
    • Journal Title

      J.Gen.Plant Pathol. 70

      Pages: 85-92

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular cloning and characterization of a cDNA encoding ent-eassa-12,15-diene synthasc, a putative diterpenoid phytonlexin biosynthetic enzyme, from suspension-cultured rice cells treatedwith a chitin elicitor.2004

    • Author(s)
      E.M.Cho et al.
    • Journal Title

      Plant.J. 37

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Two ricc GRAS family genes responsive to N-acetylchitooligosaccharide elicitor arc induced byphytoactive gibberellins : Evidence for cross talk between elicitor-and gibberellin-signaling in rice cells.2004

    • Author(s)
      R.B.Day et al.
    • Journal Title

      Plant Mol.Biol. 54

      Pages: 261-272

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization and cDNA cloning of monomeric lectins that correspond to the B-chain of a type 2 ribosome-inactivating protein from the bark of Japanese elderberry (Sambucus sieboldiana).2004

    • Author(s)
      M.A.Rojo et al.
    • Journal Title

      J.Biochem. 135

      Pages: 509-516

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Activation of Phospholipase D Induced by Hydrogen Peroxide in Suspension-cultured Rice Cells.2004

    • Author(s)
      T.Yamaguchi et al.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1261-1270

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Stemar-l3-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin.S in rice.2004

    • Author(s)
      T.Nemoto et al.
    • Journal Title

      PEBS Lett. 571

      Pages: 182-186

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] RERJI, a jasmonic acid-responsive gene from rice encodes a basic helix-loop-helix protein.2004

    • Author(s)
      K.Kiribuchi et al.
    • Journal Title

      Biochem.Biophys.Res.Comm. 325

      Pages: 857-863

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rice gene expression in response to N-acetylchitooligosaccharide elicitor : comprehensive analysis by DNA microarray with rundumly selected ESTs2003

    • Author(s)
      C.Akimoto-Tomoyama
    • Journal Title

      Plant Mol.Biol. 52

      Pages: 537-551

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of rice chitinase gene triggered by the direct injection of Ca^<2+>2003

    • Author(s)
      M.Saito
    • Journal Title

      J.Biotechnol. 105

      Pages: 537-551

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Niconana benthamiana gp9I^<pho1> homologs NbrbohA and NhrbohB participate in H_2O_2 accumulation and resistance to Phyrophthora in Jesatans2003

    • Author(s)
      H.Yoshioka
    • Journal Title

      Plant Cell 15

      Pages: 706-718

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Functional analysis of potato mitogen-actibated protein kinase kinase, StMEK12003

    • Author(s)
      S.Katou
    • Journal Title

      J.Gene.Plant Pathol 69

      Pages: 161-168

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Elicitation of primary and secondary metabolisms during defense in the potato2003

    • Author(s)
      E.Nakane
    • Journal Title

      J.Gen Plant Pathol. 69

      Pages: 378-384

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification and charcterization of two new members of the GRAS gene family in rice responsive to N-acetylchitooli gosaccharide elicitor.2003

    • Author(s)
      R.B.Day
    • Journal Title

      Biochem.Biophisic Acta 1625

      Pages: 361-370

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Actiyation of phspholypase,by N-acetylchitooligosaccharide elicitor in suspension-culimed rice cells mediates reactiveoxygen generation2003

    • Author(s)
      J.Yamaguchi
    • Journal Title

      Physiol Plant 118

      Pages: 361-370

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Rice, gene expression in response to N-acetylehitooligo- Saccharide elicitor : comprehensive analysis by DNA micro array with randomly selected ESTs.2003

    • Author(s)
      C.Akimoto-Tomiyama, et al.
    • Journal Title

      Plant Mol.Biol. 52

      Pages: 537-551

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of rice chitinase gene triggered by the direct injection of Ca'2003

    • Author(s)
      M.Saito et al.
    • Journal Title

      J.Biotechnol 105

      Pages: 537-551

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Nicotiana benthamiana gp91^<pho/> homologs NbrbohA and NbrbohB participate in H_2O_2 accumulation and resistance to Phytophtyora Infesatans2003

    • Author(s)
      H.Yoshioka et al.
    • Journal Title

      Plant Cell 15

      Pages: 706-718

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional analysis of potato mitogen-actihatcd protein kinasc kinase, StMEK12003

    • Author(s)
      S.Katou, et al.
    • Journal Title

      J.Cen.Plant Pathol 69

      Pages: 161-168

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Elicitation of primary an secondary metabolisms during defense in the potato.2003

    • Author(s)
      E.Mikan, et al.
    • Journal Title

      J.Gen Plant Pathol 69

      Pages: 378-384

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identifieantion and characterization of two new members of the GRASgene family in rice responsive to N-acetylchitooignsacclittride elicitor.2003

    • Author(s)
      R.B.Day, et al.
    • Journal Title

      Biochem.Biophisic Acta 1625

      Pages: 361-370

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Activation of phspholipase by N-acetylchitooligosaccharide elicitor in suspension-cultured rice cells mediates reactive oxygen generation.2003

    • Author(s)
      J.Yamaguchi et al.
    • Journal Title

      Physiol Plant 118

      Pages: 361-370

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Two purified oligosaccharide elicitors N-acetylchitoheptose and tetraglucosylglucocitol. derived from Magnaporhte grisea cell wall, synergistically activate biosyntheis of phytoalexin in suspension-cultued cells.2002

    • Author(s)
      T.Yamaguchi
    • Journal Title

      J.Plant Physiol 159

      Pages: 1147-1149

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] キチンオリゴ糖による植物の生体防御機構の調節2002

    • Author(s)
      渋谷直人
    • Journal Title

      バイオインダストリー 19

      Pages: 6-12

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 植物の菌類病研究の現状と将来展望-感染生理学を中心として2002

    • Author(s)
      道家紀志
    • Journal Title

      植物感染生理談話会論文集 38

      Pages: 77-88

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Large-scakale identification of elicitor-responsive genes in suspension-cultured rice cells by DNA microarray.2002

    • Author(s)
      R.B.Day
    • Journal Title

      Plant Biotech 19

      Pages: 153-155

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] High-affinity binding proteins for N-acctylchitooligosaccharide elicitor in the plasama membernnes from wheat barley and earrot cells : conserved presence and correlation b with the responsiveness to the elicitor2002

    • Author(s)
      M.Okada
    • Journal Title

      Plant Cell Physiol 43

      Pages: 505-512

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ELS5, a rice N-acetylchitooligosaccharide elicitor-responsive RING-H2 finger proein, is a ubiquitin ligase whit^ch functions in vitro in co-operation with an elieitor- respons cive ubiquitin-conjygatting enzyme, OsUBC5b.2002

    • Author(s)
      R.Takai
    • Journal Title

      Plant J. 30

      Pages: 447-445

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cloning and characterization of cDNAs for the jasmonic acid-responsive genes PRJ1 and PAJ2 in suspension-cultured rice cells.2002

    • Author(s)
      M.Sugimori
    • Journal Title

      Biotech.Biotechnol.Bioche,. 66

      Pages: 1140-1142

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Rice receptor for chitin oligosaccharide elicitor does not couple to hetrotrimenc G. protein : elicitor responses of suspension cultured rice cells from Daikoku dwarf (dl) mutants lacking a functional G-protein a-sabunit.2002

    • Author(s)
      K.Tsukada
    • Journal Title

      Physiol Plant 116

      Pages: 373-382

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Two purified oligosaccharidc elicitors, N-acetylchitoheptose and tetraglucosylglucocitol, derived from Magnaporhte grisea cell wall, synergistically activate biosynthesis of phytoalexin in suspension-cultured cells.2002

    • Author(s)
      T.Yamaguchi et al.
    • Journal Title

      J.Plant Physiol 159

      Pages: 1147-1149

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulation of plan defense mechanism by chitinorigosaccharides2002

    • Author(s)
      N.Shibuya
    • Journal Title

      Biodindustry 19

      Pages: 6-12

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Current status and perspective in research on host-fungi interaction -a special consideration on infection physiology-2002

    • Author(s)
      N.Doke
    • Journal Title

      Proceeding of plant infection physiology 39

      Pages: 77-88

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Large-scale identification of elicitor-responsive genes in suspension-cultured rice cells by DNA microarray.2002

    • Author(s)
      R.B.Day
    • Journal Title

      Plant Bioteeh. 19

      Pages: 153-155

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] High-affinity binding proteins for N-acctylchitooligosaccharide elicitor in the plasma membranes from wheat barley and carrot cells : conserved presence and correlation with the responsiveness to the elicitor.2002

    • Author(s)
      M.Okada et al.
    • Journal Title

      Plant Cell Physiol 43

      Pages: 505-512

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ELSS, a rice N-acetylchitooligosaccharide elicitor-responsive RING-H2 finger protein, is a ubiquitin ligasc whitch functions in vitro in co-operation with an elicitor-responsive ubiquitin-conjugating enzyme, OsUBC5b.2002

    • Author(s)
      R.Takai et al.
    • Journal Title

      Plant.J. 30

      Pages: 447-445

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cloning and characterization of cDNAs for the jasmonic acid-responsive genes PRJ1 and PRJ2 in suspension-cultured rice cells.2002

    • Author(s)
      M.Sugimori et al.
    • Journal Title

      Biotech.Biotechnol.Bindle 66

      Pages: 1140-1142

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rice receptor for chitin oligosaccharide elicitor does not couple to hcterotrimeric G-protein : elicitor responses of suspension cultured rice cells from Daikoku dwarf (dl) mutants lacking a functional G-protcin a-sabunit.2002

    • Author(s)
      K.Tsukad et al.
    • Journal Title

      Physi Plant. 116

      Pages: 373-382

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of a high -affinity binding protein for N-acetylchito-oligaosachauide elicitor in the plasma membrane from rice leaf and root cells.2001

    • Author(s)
      M.Okada
    • Journal Title

      J.Plant Physiol. 158

      Pages: 121-124

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of a high-affinity binding protein for N-acetylchito-oligosaccharide elicitor in the plasma membrane from rice leaf and root cells.2001

    • Author(s)
      M.Okada et al.
    • Journal Title

      J.Plant Physiol. 158

      Pages: 121-124

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Difference in the recognition of glucan elicitor signals between rice and soybean : b-glucan fragments from rice blast disease fungus Pyricularia oryzae that elicit phytoalexin biosynihesis in suspension-cultured rice cells.2000

    • Author(s)
      T.Yamaguchi
    • Journal Title

      Plant Cell Physiol. 12

      Pages: 817-826

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Polysaccaride elicitors and their receptors for plant defense response.2000

    • Author(s)
      T.Yamaguchi
    • Journal Title

      Trends Glycosci.Glycotech. 12

      Pages: 113-120

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Difference in the recognition of glucan elicitor signals between rice and soybean : β-glucan fragments from rice blast disease fungus Pyricularia oryzac that elicit phyoalcxin biosynthesis in suspension-cultured rice cells.2000

    • Author(s)
      T.Yamaguchi.et al.
    • Journal Title

      Plant Cell Physiol. 12

      Pages: 817-826

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Polysaccharide elicitors and their receptors for plant defense response2000

    • Author(s)
      T.Yamaguchi et al.
    • Journal Title

      Trends Glycosci.Glycotech. 12

      Pages: 113-120

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] The superoxide generating NADPH oxidase in plants : its molecular basis and role in induction of local and systemic induced resistance against pathogens. Genomic and Genetic Analysis of Plant Parasitism and defense (In : tsuyumu, s. Leach, JE. Shiraishi, T. and Walpert, eds.)2005

    • Author(s)
      N.Doke.
    • Total Pages
      195-206
    • Publisher
      APS Press
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 感染植物のオキシダティブバースト : その機構・機能と耐病性化への応用2005

    • Author(s)
      道家紀志
    • Total Pages
      28-34
    • Publisher
      「植物病の探求」出版会
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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