2000 Fiscal Year Annual Research Report

植物病原糸状菌の宿主特異的毒素生成と宿主応答の分子機構

Research Project

Project/Area Number	12052218
Research Institution	Tottori University
Principal Investigator	尾谷浩鳥取大学, 農学部, 教授 (50032305)
Co-Investigator(Kenkyū-buntansha)	児玉基一朗鳥取大学, 農学部, 講師 (00183343) 中島廣光鳥取大学, 農学部, 教授 (40144646)
Keywords	宿主特異的毒素 / 環状ペプチド / 宿主特異的毒素生合成遺伝子 / 宿主応答 / 葉緑体シャペロニン
Research Abstract	1.新規宿主特異的毒素の構造決定アブラナ科野菜黒すす病菌、トマト褐色輪紋病菌およびセイヨウナシbrown spot病菌では、新規宿主特異的毒素(HST)としてそれぞれAB、CCTおよびSV毒素を生産するが、これらHSTの中で、CCT毒素は、グルコースが結合した環状ペプチド(分子量2780.6)であることが明らかとなった。また、SV毒素では効率の良い単離・精製法が確立された。 2.宿主特異的毒素生成機構リンゴ斑点落葉病菌は環状ペプチドHST(AM毒素)を生成するが、環状ペプチド合成酵素遺伝子配列から設計されたPCRプライマーを用いて、AM毒素生合成遺伝子のクローニングに成功した。さらに、本遺伝子を特異的に増幅するプライマーを用いたPCRによるリンゴ斑点落葉病菌の特異的検出法が確立され、本法から、最近、新潟県で発生しているセイヨウナシ黒斑病の病原菌がリンゴ斑点落葉病菌であることが明らかとなった。 3.宿主特異的毒素応答機構リンゴ斑点落葉病感受性リンゴ品種に放射線を照射して得られた抵抗性変異体と元の感受性品種の蛋白質を二次元電気泳動法によって解析し、感受性品種のみに存在する60kDa、pI5のスポット(SA60)を見い出した。そこで、SA60を抽出し、アミノ酸配列を解析した結果、葉緑体シャペロニン(CPN60)と極めて高い相同性を示し、CPN60のホモログであることが示唆された。本菌の生産するAM毒素は、感受性リンゴ品種の葉緑体に直接作用するので、SA60がAM毒素受容に関与する因子である可能性が高い。

Research Products
(10 results)

All Other

All Publications (10 results)

[Publications] Johnson,R.D.: "Cloning and characterization of a cyclic peptide synthetase gene from Alternaria alternata apple pathotype whose product is involved in AM-toxin synthesis and pathogenicity"Molecular Plant-Microbe Interaction. 13. 742-753 (2000)
[Publications] Yamamoto,M.: "(+)-Catechin acts as an infection-inhibiting factor in strawberry leaf"Phytopathology. 90. 595-600 (2000)
[Publications] Johnson,R.D.: "A PCR-based method to specifically detect Alternaria alternata apple pathotype (A. mali), the causal agent of Alternaria blotch of apple"Phytopathology. 90. 973-976 (2000)
[Publications] 尾谷浩: "植物病原菌の宿主識別と宿主特異的毒素の作用機構に関する研究"日本植物病理学会報. 66. 63-65 (2000)
[Publications] Otani,H.: "Host recognition by plant pathogens and role of host-specific toxins"Journal of General Plant Pathology,. 66. 278-280 (2000)
[Publications] Otani,H.: "A new type of host-selective toxin, a protein produced from Alternaria brassicicola"Delivery and Perception of Pathogen Signals in Plants (Ed. by Keen,N.T.et al.),APS Press,St.Paul,U.S.A.. 68-76 (2001)
[Publications] Salamiah: "Genetic analysis of pathogenicity and host-specific toxin production of Alternaria alternata tomato pathotype by protoplast fusion"Journal of General Plant Pathology. 67. 7-14 (2001)
[Publications] Salamiah: "Construction and genetic analysis of hybrid strains between the apple and tomato pathotypes of Alternaria alternata by protoplast fusion"Journal of General Plant Pathology. (in press). (2001)
[Publications] Singh,P.: "Host-specific SV-toxin of the fungal pathogen causing brown spot of European pear"Acta Horticultulae. (in press). (2001)
[Publications] Otani,H.: "Molecular basis of host-specific toxins: host-specificity and genetic analysis in Alternaria pathogens"Proceedings of 1st Asian Conference on Plant Pathology. (in press). (2001)

2000 Fiscal Year Annual Research Report

植物病原糸状菌の宿主特異的毒素生成と宿主応答の分子機構

Principal Investigator

尾谷 浩 鳥取大学, 農学部, 教授 (50032305)

Research Products

[Publications] Johnson,R.D.: "Cloning and characterization of a cyclic peptide synthetase gene from Alternaria alternata apple pathotype whose product is involved in AM-toxin synthesis and pathogenicity"Molecular Plant-Microbe Interaction. 13. 742-753 (2000)

[Publications] Yamamoto,M.: "(+)-Catechin acts as an infection-inhibiting factor in strawberry leaf"Phytopathology. 90. 595-600 (2000)

[Publications] Johnson,R.D.: "A PCR-based method to specifically detect Alternaria alternata apple pathotype (A. mali), the causal agent of Alternaria blotch of apple"Phytopathology. 90. 973-976 (2000)

[Publications] 尾谷浩: "植物病原菌の宿主識別と宿主特異的毒素の作用機構に関する研究"日本植物病理学会報. 66. 63-65 (2000)

[Publications] Otani,H.: "Host recognition by plant pathogens and role of host-specific toxins"Journal of General Plant Pathology,. 66. 278-280 (2000)

[Publications] Otani,H.: "A new type of host-selective toxin, a protein produced from Alternaria brassicicola"Delivery and Perception of Pathogen Signals in Plants (Ed. by Keen,N.T.et al.),APS Press,St.Paul,U.S.A.. 68-76 (2001)

[Publications] Salamiah: "Genetic analysis of pathogenicity and host-specific toxin production of Alternaria alternata tomato pathotype by protoplast fusion"Journal of General Plant Pathology. 67. 7-14 (2001)

[Publications] Salamiah: "Construction and genetic analysis of hybrid strains between the apple and tomato pathotypes of Alternaria alternata by protoplast fusion"Journal of General Plant Pathology. (in press). (2001)

[Publications] Singh,P.: "Host-specific SV-toxin of the fungal pathogen causing brown spot of European pear"Acta Horticultulae. (in press). (2001)

[Publications] Otani,H.: "Molecular basis of host-specific toxins: host-specificity and genetic analysis in Alternaria pathogens"Proceedings of 1st Asian Conference on Plant Pathology. (in press). (2001)

尾谷浩鳥取大学, 農学部, 教授 (50032305)