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2005 Fiscal Year Final Research Report Summary

The resistance mechanism of plants against virus infection

Research Project

Project/Area Number 12052222
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

OKUNO Tetsuro  Kyoto University, Graduate School of Agriculture, Professor, 大学院農学研究科, 教授 (00221151)

Co-Investigator(Kenkyū-buntansha) WATANABE Yuichiro  University of Tokyo, Graduate School of Arts and Sciences, Associate Professor, 大学院・総合文化研究科, 助教授 (60183125)
Project Period (FY) 2000 – 2005
Keywordsvirus / RNA silencing / RNAi / RNA replication / attenuated virus / miRNA / Arabidopsis / Dicer
Research Abstract

Okuno's group found that to suppress Red clover necrotic mosaic virus (RCNMV) needs multiple viral components, including viral RNAs and putative RNA replicase proteins. A close relationship between the RNA elements required for negative-strand RNA synthesis and RNAi suppression suggests a strong link between the viral RNA replication machinery and the RNAi machinery. DCL1 mutants of Arabidopsis thaliana did not support RCNMV infection, suggesting DCL1 is a host component required for RCNMV infection. RCNMV preferentially interferes with the accumulation of long small-interfering RNAs (24-26 nt) in RNAi induced by a hairpin dsRNA. We propose a model in which, to replicate, RCNMV deprives the RNAi machinery of a factor that is probably a dicer-like protein, and suppresses Dicer-mediated dsRNA cleavage. They also found that the stem-loop structure that functions as a trans-activator for the RNA-mediated CP expression is critically required for RNA2 replication of RCNMV itself.
Watanabe's group found that DCL1 catalyzes at least the first and second cleavage steps in miRNA biogenesis and that double-stranded RNA-binding domains of DCL1 are involved in positioning of the cleavage sites. Our result is direct evidence that DCL1 is involved in processing of pri-and pre-miRNA. (dsRNA )-binding domains of DCL1 are involved in positioning of the cleavage sites. They also identified several proteins associated with Tobacco mosaic virus movement protein in plasmodesmata.

  • Research Products

    (15 results)

All 2005 2004

All Journal Article (13 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] The Red clover necrotic mosaic virus RNA2 trans-activator is also acis-acting RNA2 replication element.2005

    • Author(s)
      Tatsuta, M. et al.
    • Journal Title

      Journal of Virology 79

      Pages: 978-986

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Timing and Extent of Hypersensitive Response are Critical to Restrict Local and Systemic Spread of Pepper mild mottle virus in Pepper Containing the L^3 Gene.2005

    • Author(s)
      Hamada, H. et al.
    • Journal Title

      Journal of General Plant Pathology 71

      Pages: 90-94

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Discrimination of Tobamoviruses and Their Pathotypes for L gene-mediated resistance in Green Pepper (Capsicum annuum L.) by Reverse Transcription Polymerase Chain Reaction2005

    • Author(s)
      Takeuchi, S., et al.
    • Journal Title

      Journal of General Plant Pathology 71

      Pages: 60-67

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The Red clover necrotic mosaic virus RNA2 trans-activator is also a cis-acting RNA2 replication element2005

    • Author(s)
      M.Tatsuta et al.
    • Journal Title

      J.Virolrogy 79

      Pages: 978-986

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Timing and Extent of Hypersensitive Response are Critical to Restrict Local and Systemic Spread of Pepper mild mottle virus in Peppep Containing the L^3 Gene.2005

    • Author(s)
      H.Hamada et al.
    • Journal Title

      J.Gen.Plant Pathol. 71

      Pages: 90-94

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Discrimination of Tobamoviruses and Their Pathotypes. for L gene-mediated resistance in Green Pepper (Capsicum annuum L.) by Reverse Transcription Polymerase Chain Reaction2005

    • Author(s)
      S.Takeuchi at al.
    • Journal Title

      J.Gen.Plant Pathol. 71

      Pages: 60-67

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Arabidopsis micro-RNA biogenesis through Dicer-like protein functions2004

    • Author(s)
      Kurihara, Y., Watanabe, Y.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 12753-12758

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of host-specificity determinants in hetanodaviruses by using reassortants between striped jack nervous necrosis virus and sevenband grouper nervous necrosis virus.2004

    • Author(s)
      Iwamoto, T. et al.
    • Journal Title

      Journal of Virology 78

      Pages: 1256-1262

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A TMV-Cg mutant with a truncated coat protein induces cell death resembling the hypersensitive response in Arabidopsis.2004

    • Author(s)
      Kurihara, Y., Watanabe, Y.
    • Journal Title

      Molecular Cells 17

      Pages: 334-339

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Arabidopsis micro-RNA biogenesis through Dicer-like protein functions2004

    • Author(s)
      Y.Kurihara, Y.Watanabe
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 12753-12758

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of host-specificity determinants in betanodaviruses by using reassortants between striped jack nervous necrosis virus and sevenband grouper nervous necrosis virus.2004

    • Author(s)
      T.Iwamoto et al.
    • Journal Title

      J.Virol. 78

      Pages: 1256-1262

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A TMV-Cg mutant with a truncated coat protein induces ce II death resembling the hypersensitive response in Arabidopsis.2004

    • Author(s)
      Y.Kurihara, Y.Watanabe
    • Journal Title

      Molecular Cells 17

      Pages: 334-339

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] New Strategy in Bioscience : Plantvirus and Bioscience2004

    • Author(s)
      T.Okuno et al.
    • Journal Title

      Maruzen

      Pages: 250

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] バイオサイエンスの新戦略 : 植物ウイルスとバイオサイエンス2004

    • Author(s)
      奥野哲郎(その他)
    • Total Pages
      250
    • Publisher
      丸善
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 温度依存性翻訳制御ポリヌクレオチド2004

    • Inventor(s)
      奥野 哲郎ら
    • Industrial Property Rights Holder
      京都大学・発明者
    • Industrial Property Number
      特願2004-117384
    • Filing Date
      2004-04-12
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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