SYSTEMATIC FUNCTIONAL ANALYSES OF GENES IN THE DROSOPHILA GENOME

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 12202002
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: TOKYO METROPOLITAN UNIVERSITY
• Principal Investigator: AIGAKI Toshiro TOKYO METROPOLITAN UNIVERSITY, BIOLOGICAL SCIENCES, ASSOCIATE PROFESSOR, 理学研究科, 助教授 (80150879)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Shigeo RIKEN, CDB, GROUP DIRECTOR, CDB, グループデイレクター(研究職) (60183092)
• Project Period (FY): 2000 – 2004
• Keywords: Drosophila / misexpression vector / misexpression phenotype / Gene Search system / GS line / GS vector / GAL4-UAS system / Phenotvpic information

Research Abstract

Genetic approaches in Drosophila melanogaster have defined many genes that have been informative for understanding the function of their counterparts in vertebrates including humans. However, not all genes show phenotypic changes when mutated: the phenotype may be too subtle to be detected, or other genes may compensate the defects. It is estimated that approximately two-thirds of genes belong to this category. To overcome this limitation, we conducted a large-scale Insertional mutagenesis using a series of P-element based "Gene Search vector", which, when inserted in the genome, allows GAL4-dependent misexpression of the vector-flanking genes. We have generated approximately. 16,000 lines carrying a GS vector, of which 12,000 have been mapped on the genome based on the vector-flanking sequence determined by inverse-PCR or vector-PCR. We constructed a comprehensive database for GS lines, with which one can easily identify GS lines that have a
vector insertion within or nearby the gene of interest (http://gsdb.biol.metro-u.ac.jp/%7Edclust/). It also allows to search based on the name of gene, map position, or phenotypes. The collection provides opportunities to identify novel genes based on the misexpression phenotypes, which can be induced tissue-or stage-specifically depending on the GAL4 drivers to be used. Using these lines, we have identified and characterized a number of novel genes that are involved in development, cell death, innate immunity, behavior, or longevity.

Research Products

• [Journal Article] Alternative trans-splicing : a novel mode of pre-mRNA processing. (2006)
  • Author(s): Horiuchi, T., Aigaki, T.
  • Journal Title: Biol. Cell 98 Pages: 135-140
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The RING finger-scaffold protein POSH targets TAK1 to control immunity signaling in Drosophila. (2005)
  • Author(s): Tsuda, M., Langmann, C., Harden, N., Aigaki, T.
  • Journal Title: EMBO reports 6 Pages: 1082-1087
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Gain-of-function screen identifies a role of the Sec6lalpha translocon in Drosophila postmitotic neurotoxicity. (2005)
  • Author(s): Kanuka, H., Hiratou, T., Igaki, T., Kanda., H., Kuranaga, E., Sawamoto, K., Aigaki, T., Okano, H., Miura, M.
  • Journal Title: Biochim. Biophys. Acta 1726 Pages: 225-237
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification. (2005)
  • Author(s): Katsuyama, T., Sugawara, T., Tatsumi, M., Oshima, Y., Gehring, W., Aigaki, T., Kurata, S.
  • Journal Title: Proc. Natl. Acad. Sci.USA 102 Pages: 15918-15923
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The RING finger-scaffold protein POSH targets TAK1 to control immunity signalling in Drosophila. (2005)
  • Author(s): Tsuda, M., Langmann, C., Harden, N., Aigaki, T.
  • Journal Title: EMBOr 6 Pages: 1082-1087
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of a secreted form of Dally, a Drosophila glypican, induces overgrowth phenotype by affecting action range of Hedgehog. (2005)
  • Author(s): Takeo, S., Akiyama, T., Firkus, C., Aigaki, T., Nakato, H.
  • Journal Title: Dev. Biol. 284 Pages: 204-218
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth. (2005)
  • Author(s): Laviolette, M.J., Nunes, N., Peyre, J.B., Aigaki, T., Stewart, B.A.
  • Journal Title: Genetics 170 Pages: 779-792
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The slender lobes gene, identified by retarded mushroom body development, is required for proper nucleolar organization in Drosophila. (2005)
  • Author(s): Orihara-Ono, M., Suzuki, M., Saito, M., Yoda, Y., Aigaki, T., Hama, C.
  • Journal Title: Dev. Biol. 281 Pages: 121-133
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Drosophila orthologues of human b-secretase induce the secretion of a Golgi-resident transferase, heparan sulfate 6-O-sulfotransferase. (2005)
  • Author(s): Kotani, N., Kitazume, S., Kamimura, K., Takeo, S., Aigaki, T., Nakato, H., Hashimoto, Y.
  • Journal Title: J. Biochem. 137 Pages: 315-322
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparative sequences analysis of a gene-dense region among closely related species of Drosophila melanogaster. (2004)
  • Author(s): Kawahara, Y., Matsuo, T., Nozawa, M., Shin-I, T., Kohara, Y., Aigaki, T.
  • Journal Title: Genes Genetic Systems 79 Pages: 351-359
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression level of sarah, a homolog of DSCR1 is critical for ovulation and female courtship behavior in Drosophila melanogaster. (2004)
  • Author(s): Ejima, A., Tsuda, M., Takeo, S., Ishii, K., Matsuo, T., Aigaki, T.
  • Journal Title: Genetics 168 Pages: 2077-2087
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparative sequences analysis of a gene-dense region among closely related species of Drosophila melanogaster. (2004)
  • Author(s): Kawahara, Y, Matsuo, T., Nozawa, M., Shin-I, T., Kohara, Y., Aigaki, T.
  • Journal Title: Gen Genet. Sys. 79 Pages: 351-359
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Drosophila Nedd4 regulates endocytosis of Notch and suppresses its ligand-independent activation. (2004)
  • Author(s): Sakata, T., Sakaguchi, H., Tsuda, L., Higashitani, A., Aigaki, T.; Matsuno, K., Hayashi, S.
  • Journal Title: Curr. Biol. 14 Pages: 2228-2236
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Formin 3 is required for assembly of the F-actin structure that mediates tracheal fusion in Drosophila. (2004)
  • Author(s): Tanaka, H., Takasu, E., Aigaki, T., Kato, K., Hayashi, S., Nose, A.
  • Journal Title: Devel. Biol. 274 Pages: 413-425
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genetic Bases of Oxidative Stress Resistance and Life span in Drosophila. (2004)
  • Author(s): Aigaki, T., Kaneuchi, T., Matsuo, T., Seong, K-H., Togawa, T.
  • Journal Title: J. Clin. Biochem. Nut. 34 Pages: 77-83
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In vivo hyaluronan synthesis by expression of mammalian hyaluronan synthase gene in Drosophila. (2004)
  • Author(s): Takeo S., Fujise, M., Akiyama, T., Habuchi, H., Itano, N., Matsuo, T., Aigaki, T., Kimata, K., Nakato, H.
  • Journal Title: J. Biol. Chem. 279 Pages: 18920-18925
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Alternative trans-splicing of constant and variable exons of a Drosophila axon guidance gene, lola. (2003)
  • Author(s): Horiuchi, T., Giniger, E., Aigaki, T.
  • Journal Title: Genes ＆ Development 17 Pages: 2496-2501
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Alternative splicing of lola generates 19 transcription factors controlling axon guidance in Drosophila. (2003)
  • Author(s): Goeke, S., Greene, E.A., Grant, P.K., Gates, M.A., Crowner, D., Aigaki, T., Giniger, E.
  • Journal Title: Nature Neuroscience 6 Pages: 917-924
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Alternative trans-splicing of constant and variable exons of a Drosophila axon guidance gene, lola. (2003)
  • Author(s): Horiuchi, T., Giniger, E., Aigaki, T.
  • Journal Title: Genes Dev. 17 Pages: 2496-2501
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Alternative splicing of lola generates 19 transcription factors controlling axon guidance in Drosophila. (2003)
  • Author(s): Goeke, S., Greene, E.A., Grant, P.K., Gates, M.A., Crowner, D., Aigaki, T., Giniger, E.
  • Journal Title: Nat. Neurosci. 6 Pages: 917-924
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Drosophila lola encodes a family of BTB-transcription regulators with highly variable C-terminal domains containing zinc finger motifs. (2003)
  • Author(s): Ohsako, T., Horiuchi, T., Matsuo, T., Komaya, S., Aigaki, T.
  • Journal Title: Gene 311 Pages: 59-69
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Efficient method for measurement of H2O2 resistance in Drosophila. (2003)
  • Author(s): Kaneuchi, T., Togawa, T., Matsuo, T., Fuyama, Y., Aigaki, T.
  • Journal Title: Biogerontology 4 Pages: 157-165
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] (2003) Dally regulates Dpp morphogen gradient formation in the Drosophila wing. (2003)
  • Author(s): Fujise, M., Takeo, S., Kamimura, K., Matsuo, T., Aigaki, T., Izumi, S., Nakato, H.
  • Journal Title: Development 130 Pages: 151-1522
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] GETDB, a database compiling expression patterns and molecular locations of a collection of Ga14 enhancer traps. (2002)
  • Author(s): Hayashi, S., Ito, K., Sado, Y., Taniguchi, M., Takeuchi, H., Aigaki, T., Matsuzaki, F., Nakagoshi, H., Tanimura, T., Ueda, R., Uemura, T., Yoshihara, M., Goto, S.
  • Journal Title: Genesis 34 Pages: 58-61
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Overexpression of a pattern-recognition receptor, peptidoglycan-recognition protein-LE, activates imd/relish-mediated antibacterial defense and prophenoloxidase cascade in Drosophila larvae. (2002)
  • Author(s): Takehana, A., Katsuyama, T., Tamaki, Y., Oshima, Y., Takada, H., Aigaki, T., Kurata, S.
  • Journal Title: Proc. Natl. Acad. Sci.USA 99 Pages: 13705-13710
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Longevity determination genes in Drosophila melanogaster. (2002)
  • Author(s): Aigaki, T., Seong, K.H., Matsuo, T.
  • Journal Title: Mech.Age.Dev. 123 Pages: 1531-1541
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Eiger, a TNF superfamily ligand that triggers the Drosophila JNK pathway. (2002)
  • Author(s): Igaki, T., Kanda, H., Goto, Y., Kanuka, H., Kuranaga, E.Aigaki, T., Miura, M.
  • Journal Title: EMBO J. 21 Pages: 3009-3018
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Forked end : a novel transmembrane protein involved in neuromuscular specificity in Drosophila identified by gain-of-function screening (2002)
  • Author(s): Umemiya, T., Takasu, E., Takeichi, M., Aigaki, T., Nose, A.
  • Journal Title: J. Neurobiol. 51 Pages: 205-214
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The gene search system : its application to functional genomics and a screen for longevity genes in Drosophila melanogaster. (2001)
  • Author(s): Aigaki, T., Ohsako, T., Toba, G., Seong, K.-H., Matsuo, T.
  • Journal Title: Journal of Neurogenetics 15 Pages: 1-10
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Neural specific overexpression of DPOSH, Drosophila Plenty of SH3s, extends the longevity of adult flies. (2001)
  • Author(s): Seong, K.-H., Matsuo, T., Fuyama, Y., Aigaki, T.
  • Journal Title: Biogerontology 2 Pages: 271-281
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Application of the gene search system to a screen for longevity gene in Drosophila. (2001)
  • Author(s): Seong, K.-H., Ogashiwa, T., Matsuo, T., Fuyama, Y., Aigaki, T.
  • Journal Title: Biogerontology 2 Pages: 209-217
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The gene search system : its application to functional genomics and a screen for longevity genes in Drosophila melanogaster. (2001)
  • Author(s): Aigaki, T., Ohsako, T., Toba, G., Seong, K.-H., Matsuo, T.
  • Journal Title: J. Neurogenet. 15 Pages: 1-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phenotypic association of spontaneous ovulation and sexual receptivity in virgin females of Drosophila melanogaster. (2001)
  • Author(s): Ejima, A., Nakayama, S., Aigaki, T.
  • Journal Title: Behav. Genet. 31 Pages: 437-444
  • Description: 「研究成果報告書概要(欧文)」より
• [Patent(Industrial Property Rights)] 遺伝子探索ベクター隣接ゲノム配列の同定方法、及び酸化ストレス感受性変異体 (2005)
  • Inventor(s): 相垣敏郎
  • Industrial Property Rights Holder: 首都大学東京
  • Industrial Property Number: 特願2005-324980
  • Filing Date: 2005-11-09
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 寿命関連遺伝子の探索方法 (2001)
  • Inventor(s): 相垣敏郎
  • Industrial Property Rights Holder: 科学技術振興機構
  • Industrial Property Number: 特願2001-378756
  • Filing Date: 2001-12-12
  • Acquisition Date: 2003-06-24
  • Description: 「研究成果報告書概要(和文)」より