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2004 Fiscal Year Final Research Report Summary

Analysis of developmental genetic system using small teleost fish as vertebrate model systems

Research Project

Project/Area Number 12202004
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionUniversity of Tokyo (2001-2004)
National Institute of Genetics (2000)

Principal Investigator

TAKEDA Hiroyuki  University of Tokyo, Graduate School of Science, 大学院理学系研究科, 教授 (80179647)

Co-Investigator(Kenkyū-buntansha) NARUSE Kiyoshi  University of Tokyo, Graduate School of Science, 大学院理学系研究科, 講師 (50208089)
HORI Hiroshi  Nagoya University, Graduate School of Science, 大学院理学研究科, 教授 (60116663)
KUDO Akira  Tokyo Institute of Technology, Department of Biological information, 生命理工研究科, 教授 (70178002)
Project Period (FY) 2000 – 2004
Keywordsmedaka / genome / EST / Hox / mutant / development / evolution / SNP
Research Abstract

In the present study, we focused on the medaka that became an attractive model organism for the study of vertebrate early development and genome evolution. The following three groups were jointed, aiming at the establishment of comprehensive resources for medaka developmental genetics as well as structural and functional analyses of developmentally important genes in medaka.
1. Takeda and Naruse
To strengthen the study of medaka developmental genetics, Takeda's laboratory has conducted a large-scale isolation of ESTs from medaka embryos and developed tools that facilitate mutant analysis such as rapid mapping system, M-markers and microarray with 8,000 uni-genes. These resources are accelerating medaka mutant analyses and made an important contribution to the medaka genome sequencing project.
2. Hori
Hori's laboratory analyzed the organization of the entire hox gene loci. Together with the information in the Fugu genome database and in the Danio genome database, the physical maps of three fish genomes were constructed and compared one another. Not only numbers of hox genes but also the distances between the neighboring hox genes are highly similar between medaka and fugu. As for six clusters, Aa, Ab, Ba, Bb, Ca and Da that are commonly present in the three fishes, only few or no differences were found in each cluster. Thus, the hox gene sets should have been well conserved once they had been established in respective species.
3. Kudo
Kudo's laboratory has screened for mutants that are defective in organogenesis and fin regeneration in the medaka, using ethylnitrosourea (ENU) as a mutagen. A total of 386 mutagenized genomes were scored to identify 151 mutations affecting organogenesis, and 16 mutations affecting the fin regeneration were identified. In parallel, they isolated 20,000 ESTs from re-generating fins, representing about 3,000 uni-genes.

  • Research Products

    (23 results)

All 2005 2004 2002 2001 Other

All Journal Article (23 results)

  • [Journal Article] Identification of a gene required for de novo DNA methylation of the zebrafish no tail gene2005

    • Author(s)
      Shimoda, N.
    • Journal Title

      Developmental Dynamics 233

      Pages: 1509-1516

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic linkage map of medaka with polymerase chain reaction length polymorphisms2005

    • Author(s)
      Kimura, T.
    • Journal Title

      Gene 19

      Pages: 24-31

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Retinoic acid-metabolizing enzy;me Cyp26al is essential for determining territories of hindbrain and spinal cord in zebrafish2005

    • Author(s)
      Emoto, Y.
    • Journal Title

      Dev. Biol. 278

      Pages: 415-427

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic linkage map of medaka with polymerase chain reaction length polymorphisms2005

    • Author(s)
      Kimura, T.
    • Journal Title

      Gene 19(363)

      Pages: 24-31

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Retinoic acid-metabolizing enzy ; me Cyp26al is essential for determining territories of hindbrain and spinal cord in zebrafish2005

    • Author(s)
      Emoto, Y.
    • Journal Title

      Dev. Biol. 278

      Pages: 415-427

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Large-scale isolation of ESTs from medaka embryos and its application to medaka developmental genetics2004

    • Author(s)
      Kimura, T.
    • Journal Title

      Mechanisms of Development 121

      Pages: 915-932

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Early fin primordia of zebrafish larvae regenerate by a similar growth control mechanism with adult regeneration2004

    • Author(s)
      Kawakami A.
    • Journal Title

      Developmental Dynamics 231(4)

      Pages: 693-699

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A mutation in the gene for delta-aminolevulinic acid dehydratase (ALAD) causes hypochromic anemia in the medaka, Oryzias latipes2004

    • Author(s)
      Sakamoto, D.
    • Journal Title

      Mechanisms of Development 121

      Pages: 747-752

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization of mutations affecting embryonic hematopoiesis in the medaka, Oryzias latipes2004

    • Author(s)
      Tanaka, K.
    • Journal Title

      Mechanisms of Development 121

      Pages: 739-746

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Large-scale analysis of the genes involved in fin regeneration and blastema formation in the medaka, Oryzias latipes2004

    • Author(s)
      Katogi, R.
    • Journal Title

      Mechanisms of Development 121

      Pages: 861-872

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The novel medaka transglutaminase gene is expressed in developing yolk veins2004

    • Author(s)
      Koh, D.
    • Journal Title

      Gene Exp. Patterns. 4

      Pages: 263-266

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Zebrafish periostin is required for the adhesion of muscle fiber bundles to the myoseptum and for the differentiation of muscle fibers2004

    • Author(s)
      Kudo, H.
    • Journal Title

      Developmental Biolology 267

      Pages: 473-487

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Medaka genomics : a bridge between mutant phenotypes and gene function2004

    • Author(s)
      Naruse, K.
    • Journal Title

      Mechanisms of Development 121

      Pages: 619-628

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tbx24, a novel T-box gene, is mutated in the zebrafish somite segmentation mutant fused somites2002

    • Author(s)
      Nikaido, M.
    • Journal Title

      Nature Genetics 31

      Pages: 5-16

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] DMY is a Y-specific DM-domain gene required for male development in the medaka fish2002

    • Author(s)
      Matsuda M.
    • Journal Title

      Nature 417(6888)

      Pages: 559-563

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] DMY is a Y-specific DM-domain gene required for male development in the medaka fish2002

    • Author(s)
      Matsuda, M.
    • Journal Title

      Nature 417(6888)

      Pages: 559-563

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The Tol2 transposable element of the medaka fish : an active DNA-based element naturally occurring in a vertebrate genome. (Review)2001

    • Author(s)
      Koga A.
    • Journal Title

      Genes Genet Syst. 76(1)

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of the endoderm and gut in medaka, Oryzias latipes

    • Author(s)
      Kobayashi, D.
    • Journal Title

      Development, Growth and Differentiation (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transgenic analysis of medaka mesp-b enhancer in somitogenesis

    • Author(s)
      Terasaki, H.
    • Journal Title

      Development, Growth and Differentiation (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes

    • Author(s)
      Kurosawa, G.
    • Journal Title

      Gene (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of the endoderm and gut in medaka, Oryzias latipes

    • Author(s)
      Kobayashi, D.
    • Journal Title

      Development, Growth and Differentiation (In press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transgenic analysis of medaka mesp-b enhancer in somitogenesis

    • Author(s)
      Terasaki, H.
    • Journal Title

      Development, Growth and Differentiation (In press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes

    • Author(s)
      Kurosawa, G.
    • Journal Title

      Gene (in press)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27   Modified: 2021-12-17  

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