2004 Fiscal Year Final Research Report Summary
Identification of susceptible genes for essential hypertension
| Project/Area Number |
12203001
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Graduate School of Medicine, Chiba university (2002-2004)
Asahikawa Medical College (2000-2001) |
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Principal Investigator |
HATA Akira Chiba University, Graduate School of Medicine, Professor, 大学院医学研究院, 教授 (00244541)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUCHI Kenjiro Asahikawa Medical College, Professor, 医学部, 教授 (30045455)
INOUE Ituro The University of Tokyo, The Institute of Medical Science, Associate Professor, 医科学研究所, 客員助教授 (00192500)
MAKITA Yoshio Asahikawa Medical College, Assistant Professor, 医学部, 助手 (20271778)
SUGIYAMA Takuro Asahi life Foundation, The Institute of common diseases of adulthood, Laboratory Head, 検査部, 部長
SHIWAKU Kiminori Shimane University, Faculty of Medicine, 医学部, 助教授 (10108384)
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| Project Period (FY) |
2000 – 2004
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| Keywords | essential hypertension / susceptible genes / haplotype / haplotype association study / SNPs |
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| Research Abstract |
To identify susceptible genes for essential hypertension, following four steps were conducted. 1. sample collection from all over Japan, 2. selection of candidate genes, 3. haplotype construction of the candidate genes, 4. haplotype association study of candidate genes.
For sample collection, clinical cardiologists, epidemiologists, doctors working on regional health check-up were recruited from all over Japan. Thanks to them, a total of 1600 samples from clinics and 2200 samples from epidemiological population in the region have been obtained. Of these samples, a total of 200 cases and 200 controls were extracted to initial genotyping. Candidate genes were selected from two sources. One was derived from the human homologue genes that significantly increased or decreased expression in the mouse kidneys after more than three months of high salt diet detected by way of microarray analysis. The other was extracted from the reported genes in literature that expressed mainly in human kidney. A total of 121genes were assigned for candidates. Haplotype was successfully constructed in 73 genes with SNPs from Japanese SNP database (J-SNP). Then SNPs of the 73 genes were genotyped with 888 hypertensive cases and 840 normotensive controls (samples from Ehime, Osaka and Nippon Universities were added to ours). Both contingency table method and haplotype inference method were employed to haplotype association study. Finally ten genes have been survived through the screening steps. Of these, three candidate genes have been analyzed now.
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