Genome analysis of the cellular slime molds to understand the genetic system controlling cell differentiation

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 12206001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Tsukuba
• Principal Investigator: URUSHIHARA Hideko University of Tsukuba, Graduate school of Life and Environmental sciences, Professor, 大学院生命環境科学研究科, 教授 (00150087)
• Co-Investigator(Kenkyū-buntansha): TAKAKA Yoshimasa University of Tsukuba, Graduate school of Life and Environmental sciences, Professor, 大学院生命環境科学研究科, 教授 (80091908) ; MORIO Takahiro University of Tsukuba, Graduate school of Life and Environmental sciences, Assistant Professor, 大学院生命環境科学研究科, 助手 (10292509) ; MAEDA Mineko Osaka University, Graduate School of Sciences, Associate Professor, 大学院理学研究科, 助教授 (70029700) ; OCHIAI Hiroshi Hokkaido University, Graduate School of Sciences, Professor, 大学院理学研究科, 教授 (10002122)
• Project Period (FY): 2000 – 2004
• Keywords: Dictyostelium discoideum / EST analysis / Cell differentiation / Gene expression / Microarray analysis / transcription factors / Upstream sequences / Cis-elements

Research Abstract

The cellular slime mold, Dictyostelium discoideum, was used to understand the genetic control of development and differentiation. This organism normally proliferates as unicellular amoebae but gathers and constructs multicellular structures upon starvation. First, large scale analysis of cDNAs was performed to establish the genetic resources. Fourteen cDNA libraries were constructed from the cells at 5 developmental stages, and the nucleotide sequences of 95,884 clones, including 72,703 full-length ones, were determined. The sequences were assembled into 6,790 independent genes, which corresponded to 54% of the predicted 12,500 genes. These resources were then used for reverse-genetic and bioinformatic analyses. The following results were obtained.
1. Gene expression patterns during normal development were obtained showing occurrence of the overall drastic changes in expressed genes after cell aggregation. Whole mount in situ hybridization revealed the fine pattern formation in the prestalk region.
2. Two hundred and sixty genes were extracted to encode probable transcription factors, and precise expression patterns were obtained by real-time PCR for 40 of them. Thirteen genes were disrupted.
3. The Genetic network among 9 probable transcription factors was obtained using the S-system calculation based on the gene expression patterns during development in the wild type and disruption mutants.
4. Upstream promoter sequences were obtained by aligning the full-length cDNA and genome sequences, and candidate cis regulatory elements were extracted referring to the expression specificities.

Research Products

• [Journal Article] Dictyostelium transcriptional host cell response upon infection with Legionella. (2006)
  • Author(s): Farbrother, P.
  • Journal Title: Cell. Microbiol. (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A small GTPase RacF2 affects sexual cell fusion and asexual development in Dictyostelium discoideum through the regulation of cell adhesion (2006)
  • Author(s): Muramoto, T.
  • Journal Title: Dev. Growth Differ. (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evidence that the Dictyostelium STAT protein Dd-STATa plays a role in the differentiation of inner basal disc cells and identification of a promoter element essential for expression in these cells. (2005)
  • Author(s): Shimada, N.
  • Journal Title: Differentiation 73 Pages: 50-60
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] On the development of an analysis system for upstream sequences in Dictyostelium discoideum genome. (2005)
  • Author(s): Kobayashi, N.
  • Journal Title: コンピュータソフトウェア 22 Pages: 167-172
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Reverse genetic analyses of gamete-enriched genes revealed a novel regulator of the cAMP signaling pathway in Dictyostelium discoideum. (2005)
  • Author(s): Muramoto, T.
  • Journal Title: Mech. Dev. 122 Pages: 733-743
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The genome of the social amoeba Dictyostelium discoideum. (2005)
  • Author(s): Eichinger, L.
  • Journal Title: Nature 435 Pages: 43-57
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A comparative sequence analysis reveals a common GBD/FH3-FH1-FH2-DAD architecture in formins from Dictyostelium, fungi and metazoa. (2005)
  • Author(s): Rivero, F.M.T.
  • Journal Title: BMC Genom. 6 Pages: 28
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evidence that the Dictyostelium STAT protein Dd-STATa plays a role in the differentiation of inner basal disc cells and identification of a promoter element essential for expression in these cells. (2005)
  • Author(s): Shimada, N., Kawata, T., Maruo, T., Maeda, M., Urushihara, H.
  • Journal Title: Differentiation 73 Pages: 50-60
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] On the development of an analysis system for upstream sequences in Dictyostelium discoideum genome. (2005)
  • Author(s): Kobayashi, N., Marin, M., Tanaka, Y., Urushihara, H.
  • Journal Title: Computor Softwear 22(3) Pages: 167-172
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Reverse genetic analyses of gamete-enriched genes revealed a novel regulator of the cAMP signalling pathway in Dictyostelium discoideum. (2005)
  • Author(s): Muramoto, T., Takeda, S., Furuya, Y., Urushihara, H.
  • Journal Title: Mech.Dev. 122 Pages: 733-743
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The genome of the social amoeba Dictyostelium discoideum. (2005)
  • Author(s): Eichinger, L., Pachebat, J.A., Glockner, G, Rajandream, M.-A., Sucgang, R., Berriman, M., Song, J., Olsen, R., Szafranski, K., Xu, Q.et al.
  • Journal Title: Nature 435 Pages: 43-57
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A comparative sequence analysis reveals a common GBD/FH3-FH1-FH2-DAD architecture in formins from Dictyostelium, fungi and metazoa. (2005)
  • Author(s): Rivero, F.M.T., Meyer, A.K., Urushihara, H., Uyeda, T.Q., Kitayama, C
  • Journal Title: BMC Genom. 6(1) Pages: 28
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An orderly retreat : dedifferentiation is a regulated process. (2004)
  • Author(s): Katoh, M.
  • Journal Title: Proc. Natl. Acad. Sci. USA 101 Pages: 7005-7010
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analyses of cDNAs from growth and slug stages of Dictyostelium discoideum. (2004)
  • Author(s): Urushihara, H.
  • Journal Title: Nuc. Acids Res. 32 Pages: 1647-1653
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Extracellular matrix family proteins that are potential targets of Dd-STATa in Dictyostelium discoideum. (2004)
  • Author(s): Shimada, N.
  • Journal Title: J. Plant Res. 117 Pages: 345-353
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridization. (2004)
  • Author(s): Shimada, N.
  • Journal Title: Int. J. Dev. Biol. 48 Pages: 679-682
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Defect in peroxisomal multifunctional enzyme MFE1 affects cAMP relay in Dictyostelium. (2004)
  • Author(s): Matsuoka, S.
  • Journal Title: Dev. Growth Differ. 45 Pages: 195-199
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Control of cell-type proportioning in Dictyostelium discoideum by differentiation-inducing factor as determined by in situ hybridization. (2004)
  • Author(s): Maruo, T.
  • Journal Title: Eukaryot. Cell 3 Pages: 1241-1248
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An orderly retreat : dedifferentiation is a regulated process. (2004)
  • Author(s): Katoh, M., Shaw, C., Van Driessche, N., Xu, Q., Morio, T., Kuwayama, H., Obara, S., Urushihara, H., Tanaka, Y., Shaulsky, G.
  • Journal Title: Proc.Natl.Acad.Sci.USA 101 Pages: 7005-7010
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Analyses of cDNAs from growth and slug stages of Dictyostelium discoideum. (2004)
  • Author(s): Urushihara, H., Morio, T., Saito, T., Kohara, Y., Kouriki, E., Ochiai, H., Maeda, M., Williams, J.G, Takeuchi, I., Tanaka, Y.
  • Journal Title: Nuc.Acids Res. 32 Pages: 1647-1653
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Extracellular matrix family proteins that are potential targets of Dd-STATa in Dictyostelium discoideum. (2004)
  • Author(s): Shimada, N., Nishino, K., Maeda, M., Urushihara, H., Kawata, T.
  • Journal Title: J.Plant Res. 117 Pages: 345-353
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridization. (2004)
  • Author(s): Shimada, N., Maeda, M., Urushihara, H., Kawata, T.
  • Journal Title: Int.J.Dev.Biol. 48 Pages: 679-682
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Defect in peroxisomal multifunctional enzyme MFE1 affects cAMP relay in Dictyostelium (2004)
  • Author(s): Matsuoka, S., Kuwayama, H., Ikeno, D., Oyama, M., Maeda, M.
  • Journal Title: Dev.Growth Differ. 45 Pages: 195-199
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Control of cell-type proportioning in Dictyostelium discoideum by differentiation-inducing factor as determined by in situ hybridization. (2004)
  • Author(s): Maruo, T., Sakamoto, S., Iranfar, N., Fuller, D., Morio, T., Urushihara, H., Tanaka, Y., Maeda, M., F.Loomis, W.F.
  • Journal Title: Eukaryot.Cell 3 Pages: 1241-1248
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Changing patterns of gene expression in prestalk cell subtypes of Dictyostelium recognized by in situ hybridization with genes from microarray analyses. (2003)
  • Author(s): Maeda, M.
  • Journal Title: Eukaryot. Cell 2 Pages: 627-637
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family, affects fatty acid metabolism necessary for morphogenesis in Dictyostelium spp. (2003)
  • Author(s): Matsuoka, S.
  • Journal Title: Eukaryot. Cell 2 Pages: 638-645
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Construction of a gamete-enriched gene pool and RNAi- mediated functional analysis in Dictyostelium discoideum. (2003)
  • Author(s): Muramoto, T.
  • Journal Title: Mech. Dev. 120 Pages: 965-975
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and characterization of novel calcium-binding proteins of Dictyostelium and their spatial expression patterns during development. (2003)
  • Author(s): Sakamoto, S.
  • Journal Title: Dev. Growth Differ. 45 Pages: 507-514
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A novel gene trap method using terminator-REMI and 3'rapid amplification of cDNA ends (RACE) in Dictyostelium. (2003)
  • Author(s): Takeda, K.
  • Journal Title: Gene 312 Pages: 321-333
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A STAT regulated, stress-induced signalling pathway in Dictyostelium. (2003)
  • Author(s): Araki, T.
  • Journal Title: J. Cell Sci. 116 Pages: 2907-2915
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Changing patterns of gene expression in prestalk cell subtypes of Dictyostelium recognized by in situ hybridization with genes from microarray analyses. (2003)
  • Author(s): Maeda, M., Sakamoto, S., Iranfar, N., Fuller, D., Maruo, T., Ogihara, S., Morio, T., Urushihara, H., Tanaka, Y., Loomis, W.L.
  • Journal Title: Eukaryot.Cell 2 Pages: 627-637
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family, affects fatty acid metabolism necessary for morphogenesis in Dictyostelium spp. (2003)
  • Author(s): Matsuoka, S., Saito, T., Kuwayama, H., Morita, N., Ochiai, H., Maeda, M.
  • Journal Title: Eukaryot.Cell 2 Pages: 638-645
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum. (2003)
  • Author(s): Muramoto, T., Suzuki, K., Shimizu, H., Kohara, Y., Kohriki, E., Obara, S., Tanaka, Y., Urushihara, H.
  • Journal Title: Mech.Dev. 120 Pages: 965-975
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification and characterization of novel calcium-binding proteins of Dictyostelium and their spatial expression patterns during development. (2003)
  • Author(s): Sakamoto, S., Nishino, K., Tomisako, M., Kuwayama, H., Tanaka, Y., Suetake, I., Tajima, S., Ogihara, S., Coukell, B., Maeda, M.
  • Journal Title: Dev.Growth Differ. 45 Pages: 507-514
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel gene trap method using terminator-REMI and 3' rapid amplification of cDNA ends (RACE) in Dictyostelium. (2003)
  • Author(s): Takeda, K., Saito, T., Tanaka, T., Morio, T., Maeda, M., Tanaka, Y., Ochiai, H.
  • Journal Title: Gene 312 Pages: 321-333
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A STAT regulated, stress-induced signalling pathway in Dictyostelium. (2003)
  • Author(s): Araki, T., Tsujioka, M., Abe, A., Fukuzawa, M., Meima, M., Schaap, P., Morio, T., Urushihara, H., Katoh, M., Maeda, M., Tanaka, Y., Takeuchi, I., Williams, J.G
  • Journal Title: J.Cell Sci. 116 Pages: 2907-2915
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. (2002)
  • Author(s): Kuwayama, H.
  • Journal Title: Nuc. Acids Res. 30 Pages: e2
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A transcriptional profile of multicellular development in Dictyostelium discoideum. (2002)
  • Author(s): Van Driessche, N.
  • Journal Title: Development 129 Pages: 1543-1552
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A novel PCR-mediated method for one tube generation of a gene disruption construct. (2002)
  • Author(s): Kuwayama, H.
  • Journal Title: Biotech. Lett. 24 Pages: 1307-1312
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Syntaxin 7, syntaxin 8, Vtil and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum. (2002)
  • Author(s): Bogdanovic, A.
  • Journal Title: Biochem. J. 368 Pages: 29-39
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Functional Genomics of the social amoebae, Dictyostelium discoideum. (2002)
  • Author(s): Urushihara, H.
  • Journal Title: Mol. Cells 13 Pages: 1-4
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. (2002)
  • Author(s): Kuwayama, H., Obara, O., Morio, T., Katoh, M., Urushihara, H., Tanaka, Y.
  • Journal Title: Nuc.Acids Res. 30 Pages: e2
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A transcriptional profile of multicellular development in Dictyostelium discoideum. (2002)
  • Author(s): Van Driessche, N., Shaw, C., Katoh, M., Morio, T., Sucgang, R., Ibarra, M., Kuwayama, H., Saito, T., Urushihara, H., Maeda, M., Takeuchi, I., Ochiai, H., Eaton, W., Tollett, J., Halter, J., Kuspa, K., Tanaka, Y., Shaulsky, G.
  • Journal Title: Development 129 Pages: 1543-1552
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel PCR-mediated method for one tube generation of a gene disruption construct. (2002)
  • Author(s): Kuwayama, H., Obara, S., Morio, T., Katoh, M., Kuhara, K., Tanaka, Y.
  • Journal Title: Biotech.Lett. 24 Pages: 1307-1312
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum. (2002)
  • Author(s): Bogdanovic, A., Bennett, N., Kieffer, S., Louwagie, M., Morio, T., Garin, J., Satre, M., Bruckert, F.
  • Journal Title: Biochem.J. 368 Pages: 29-39
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional Genomics of the social amoebae, Dictyostelium discoideum. (2002)
  • Author(s): Urushihara, H.
  • Journal Title: Mol.Cells 13 Pages: 1-4
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification and characterization of two penta-EF-hand Ca2+-binding proteins in Dictyostelium discoideum. (2001)
  • Author(s): Ohkouchi, S.
  • Journal Title: J. Biochem. 130 Pages: 207-215
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] ORI-GENE : gene classification based on the evolutionary tree. (2001)
  • Author(s): Mizuno, H.
  • Journal Title: Bioinformatics 17 Pages: 167-173
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium development. (2001)
  • Author(s): Tsujioka, M.
  • Journal Title: Dev. Growth ＆ Differ. 43 Pages: 275-283
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] FebA : a gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum. (2001)
  • Author(s): Morio, T.
  • Journal Title: Biochim. Biophys. Acta 1519 Pages: 65-69
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and characterization of two penta-EF-hand Ca2+-binding proteins in Dictyostelium discoideum. (2001)
  • Author(s): Ohkouchi, S., Nishio, K., Maeda, M., Hitomi, K., Adachi, H., Maki, M.
  • Journal Title: J.Biochem. 130 Pages: 207-215
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] ORI-GENE : gene classification based on the evolutionary tree. (2001)
  • Author(s): Mizuno, H., Tanaka, Y., Nakai, K., Sarai, A.
  • Journal Title: Bioinformatics 17(2) Pages: 167-73
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium development. (2001)
  • Author(s): Tsujioka, M., Yokoyama, M., Nishino, K., Kuwayama, H., Morio, T., Katoh, M., Urushihara, H., Saito, T., Ochiai, H., Tanaka, Y., Takeuchi, I., Maeda, M.
  • Journal Title: Dev.Growth ＆ Differ. 43 Pages: 275-283
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] FebA : a gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum. (2001)
  • Author(s): Morio, T., Yasukawa, H., Urushihara, H., Saito, T., Ochiai, H., Takeuchi, I., Maeda, M., Tanaka, Y.
  • Journal Title: Biochim.Biophys.Acta 1519 Pages: 65-69
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A second functional delta-5 fatty acid desaturase in the cellular slime mold Dictyostelium discoideum. (2000)
  • Author(s): Saito, T.
  • Journal Title: Eur. J. Biochem. 267 Pages: 1813-1818
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Loss of a member of the aquaporin gene family, aqpA affects spore dormancy in Dictyostelium. (2000)
  • Author(s): Mitra, B.N.
  • Journal Title: Gene 251 Pages: 131-139
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Developmental changes in the spatial expression of genes involved in myosin function in Dictyostelium. (2000)
  • Author(s): Maeda, M.
  • Journal Title: Dev. Biol. 223 Pages: 114-119
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A syntaxin 7 homologue is present in Dictyostelium discoideum endosomes and controls their homotypic fusion. (2000)
  • Author(s): Bogdanovic, A.
  • Journal Title: J Biol. Chem. 275 Pages: 36691-36697
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A second functional delta-5 fatty acid desaturase in the cellular slime mold Dictyostelium discoideum. (2000)
  • Author(s): Saito, T., Morio, T., Ochiai, H.
  • Journal Title: Eur.J.Biochem. 267 Pages: 1813-1818
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Loss of a member of the aquaporin gene family, aqpA affects spore dormancy in Dictyostelium. (2000)
  • Author(s): Mitra, B.N., Yoshino, R., Morio, T., Yokoyama, M., Maeda, M., Urushihara, H., Tanaka, Y.
  • Journal Title: Gene 251 Pages: 131-139
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Developmental changes in the spatial expression of genes involved in myosin function in Dictyostelium. (2000)
  • Author(s): Maeda, M., Kuwayama, H., Yokoyama, M., Nishino, K., Morio, T., Urushihara, H., Katoh, M., Tanaka, Y., Saito, T., Ochiai, H., Takemoto, K., Yasukawa, H., Takeuchi, I.
  • Journal Title: Dev. Biol. 223 Pages: 114-119
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A syntaxin 7 homologue is present in Dictyostelium discoideum endosomes and controls their homotypic fusion. (2000)
  • Author(s): Bogdanovic, A., Bruckert, F., Morio, T., Satre, M.
  • Journal Title: J.Biol.Chem. 275(47) Pages: 36691-36697
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dictyostelium transcriptional host cell response upon infection with Legionella.
  • Author(s): Farbrother, P., Wagner, C., Na, J., Budi, N.A., Tunggal, B., Morio, T., Urushihara, H., Tanaka, Y., Schleicher, M., Steinert, M., Eichinger, L.
  • Journal Title: Cell.Microbiol. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A small GTPase RacF2 affects sexual cell fusion and asexual development in Dictyostelium discoideum through the regulation of cell adhesion.
  • Author(s): Muramoto, T., Urushihara, H.
  • Journal Title: Dev.Growth Differ. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The cDNA Sequencing Project.
  • Author(s): Urushihara, H., Morio, T., Tanaka, Y.
  • Journal Title: Methods in Molecular Biology : Dictyostelium discoideum. (eds. Eichinger, L. andRivero, F.) (Humana Press) (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] The cDNA Sequencing Project. In : Methods in Molecular Biology : Dictyostelium discoideum. (2006)
  • Author(s): Urushihara, H.
  • Publisher: Humana Press(印刷中)
  • Description: 「研究成果報告書概要(和文)」より