2003 Fiscal Year Annual Research Report

枯草菌転写制御ネットワークの全体像の解明

Research Project

Project/Area Number	12206010
Research Institution	Fukuyama University
Principal Investigator	藤田泰太郎福山大学, 生命工学部, 教授 (40115506)
Co-Investigator(Kenkyū-buntansha)	関口順一信州大学, 繊維学部, 教授 (80111053) 吉田健一福山大学, 助教授 (20230732) 三輪泰彦福山大学, 助教授 (00219833) 定家義人埼玉大学, 理学部, 教授 (40000252) 田中暉夫東海大学, 海洋学部, 教授 (10236606)
Keywords	枯草菌 / 転写制御 / ネットワーク / DNAマイクロアレイ / シグマ因子 / 2成分制御系 / HTH蛋白質 / レギュロン
Research Abstract	DNA結合転写制御因子レギュロンのDNAマイクロアレイ解析結果に基づく、個々レギュロンの機能解析を体系的に進めた。シグマ因子の支配するレギュロンの機能解析では、ECFシグマ因子の網羅的解析をほぼ終了させることができた。2成分制御系レギュロンの網羅的機能解析を15年度よりの2年間で終了させる予定である。本年度解析の進んだのは、YcbA-YcbB、YccG-YccH、YclK-YclJ、YdfH-YdfI、YhcY-YhcZ、YrkQ-YrkP、YvrG-YvrHである。これらの2成分制御系のうち応答制御因子の認識配列の特定まで解析が進められたのは、YccH、YclJ、YdfI、YrkP、YvrHである。また、YcbA-YcbBがグルタミンの取り込みと代謝に関与し、YvrG-YvrHのセンシングに抗生物質のアジスロマイシンが関係しているとの結果を得た。約200種類のHTH転写制御因子の支配するレギュロンの網羅的機能解析は、薬剤耐性遺伝子のHTH転写制御因子を多く含む、MarR、TetRとMerRファミリーの制御因子の支配するレギュロンの解析を優先して進めた。既にMarR22因子、TetR19因子およびMerR6因子のタンパク生産系を構築した。TnrAレギュロンの解析に続き、LmrAとYvmBレギュロンの標的転写単位の同定に成功した。現在、19の制御因子の少なくとも一つの主要な標的を見出している。さらに、それぞれが結合する配列のうち9因子のシス配列を定めることに成功した。また、枯草菌の基幹代謝制御因子の多重制御をうけるilv-leuオペロンの発現制御の解析により、炭素代謝、窒素代謝、緊縮制御が密接に連動している事を明らかにしつつある。この連動の本体の解明を進め、代謝制御ネットワークを統合的に捉えられるようにする。

Research Products
(16 results)

All Other

All Publications (16 results)

[Publications] Hideaki Nanamiya: "Involvement of ClpX protein in the post-transcriptional regulation of a competence specific transcription factor, ComK protein, of Bacillus subtilis"Journal of Biochemistry (Tokyo). 133(3). 295-302 (2003)
[Publications] Kei Asai: "DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family"FEMS Microbiology Letters. 220(1). 155-160 (2003)
[Publications] Kazuo Kobayashi: "Essential Bacillus subtilis genes"Proceedings of National Acadmy of Science of the United States of America. 100(8). 4678-4683 (2003)
[Publications] Tasuya Fukushima: "Transcriptional, functional and cytochemical analyses of the veg gene in Bacillus subtilis"Journal of Biochemistry (Tokyo). 133(4). 475-483 (2003)
[Publications] Ken-ichi Yoshida: "Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box"Molecular Microbiology. 49(1). 157-165 (2003)
[Publications] Shouji Watanabe: "Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis : possible cooperation of mtl and gut operons"Journal of Bacteriology. 185(16). 4816-4824 (2003)
[Publications] Mitsuo Ogura: "Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis"Molecular Microbiology. 49(6). 1685-1697 (2003)
[Publications] Thiery Doan: "The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate"Microbiology. 149(9). 2331-2343 (2003)
[Publications] Ritsuko Kuwana: "Bacillus subtilis spoVIF (yjcC) gene, involved in coat assembly and spore resistance"Microbiology. 149(10). 3011-3021 (2003)
[Publications] Hideki Yamamoto: "Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases"Journal of Bacteriology. 185(22). 6666-6677 (2003)
[Publications] Atsuhiro Arai: "Inhibition of Bacillus subtilis aprE expression by lincomycin at the posttranscriptional level through inhibition of ppGpp synthesis"Journal of Biochemistry (Tokyo). 134(5). 691-697 (2003)
[Publications] Shigeo Tojo: "Organization and expression of the Bacillus subtilis sigY Operon"Journal of Biochemistry (Tokyo). 134(6). 935-946 (2003)
[Publications] Masanao Oda: "Analysis of HutP-dependent transcription antitermination in the Bacillus subtilis hut operon : identification of HutP binding sites on but antiterminator RNA and the involvement of the N-terminus of HutP in binding of HutP to the antiterminator RNA"Molecular Microbiology. 51(4). 1155-1168 (2004)
[Publications] Fumitaka Kawai: "Cardiolipin domains in Bacillus subtilis Marburg membranes"Journal of Bacteriology. 186(5). 1475-1483 (2004)
[Publications] Masakuni Serizawa: "Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses"Gene. (in press).
[Publications] Ken-ichi Yoshida: "The fifth gene of the iol operon of Bacillus subtilis, iolE, encodes 2-keto-myo-inositol hydratase"Microbiolology. (in press).

2003 Fiscal Year Annual Research Report

枯草菌転写制御ネットワークの全体像の解明

Principal Investigator

藤田 泰太郎 福山大学, 生命工学部, 教授 (40115506)

Research Products

[Publications] Hideaki Nanamiya: "Involvement of ClpX protein in the post-transcriptional regulation of a competence specific transcription factor, ComK protein, of Bacillus subtilis"Journal of Biochemistry (Tokyo). 133(3). 295-302 (2003)

[Publications] Kei Asai: "DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family"FEMS Microbiology Letters. 220(1). 155-160 (2003)

[Publications] Kazuo Kobayashi: "Essential Bacillus subtilis genes"Proceedings of National Acadmy of Science of the United States of America. 100(8). 4678-4683 (2003)

[Publications] Tasuya Fukushima: "Transcriptional, functional and cytochemical analyses of the veg gene in Bacillus subtilis"Journal of Biochemistry (Tokyo). 133(4). 475-483 (2003)

[Publications] Ken-ichi Yoshida: "Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box"Molecular Microbiology. 49(1). 157-165 (2003)

[Publications] Shouji Watanabe: "Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis : possible cooperation of mtl and gut operons"Journal of Bacteriology. 185(16). 4816-4824 (2003)

[Publications] Mitsuo Ogura: "Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis"Molecular Microbiology. 49(6). 1685-1697 (2003)

[Publications] Thiery Doan: "The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate"Microbiology. 149(9). 2331-2343 (2003)

[Publications] Ritsuko Kuwana: "Bacillus subtilis spoVIF (yjcC) gene, involved in coat assembly and spore resistance"Microbiology. 149(10). 3011-3021 (2003)

[Publications] Hideki Yamamoto: "Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases"Journal of Bacteriology. 185(22). 6666-6677 (2003)

[Publications] Atsuhiro Arai: "Inhibition of Bacillus subtilis aprE expression by lincomycin at the posttranscriptional level through inhibition of ppGpp synthesis"Journal of Biochemistry (Tokyo). 134(5). 691-697 (2003)

[Publications] Shigeo Tojo: "Organization and expression of the Bacillus subtilis sigY Operon"Journal of Biochemistry (Tokyo). 134(6). 935-946 (2003)

[Publications] Fumitaka Kawai: "Cardiolipin domains in Bacillus subtilis Marburg membranes"Journal of Bacteriology. 186(5). 1475-1483 (2004)

[Publications] Masakuni Serizawa: "Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses"Gene. (in press).

[Publications] Ken-ichi Yoshida: "The fifth gene of the iol operon of Bacillus subtilis, iolE, encodes 2-keto-myo-inositol hydratase"Microbiolology. (in press).

藤田泰太郎福山大学, 生命工学部, 教授 (40115506)