2000 Fiscal Year Annual Research Report

ナメクジにおける学習特異的蛋白質LAPS18の機能解析と連合学習の分子神経機構

Research Project

Project/Area Number	12210048
Research Institution	The University of Tokyo
Principal Investigator	桐野豊東京大学, 大学院・薬学系研究科, 教授 (10012668)
Co-Investigator(Kenkyū-buntansha)	渡辺恵東京大学, 大学院・薬学系研究科, 助手 (80302610)
Keywords	ナメクジ / 連合学習 / 免疫組織化学 / LAPS18 / differential display法 / 初代培養神経細胞 / 成長円錐 / 神経回路
Research Abstract	ナメクジにおいて学習特異的に発現量が増大する蛋白をdifferential display法により発見し,LAPS18と名付けた.本年度の研究では,LAPS18の発現パターンの解析を行った.LAPS18特異的抗体を作成し,ニンジンとキニジンを用いて条件付けしたナメクジの脳切片におけるLAPS18の分布をみると,コントロールの脳ではLAPS18は嗅覚中枢である前脳の細胞体層に局在していたのに対し,条件付けした脳では前脳神経突起層において著しいLAPS18の増大を示した.次にナメクジ前脳の初代培養神経を用いて,LAPS18の細胞内分布を調べた.その結果,permeabilize処理を行わない細胞でも細胞表面が染色されたことから,LAPS18は細胞表面に存在することが明らかになった.特に神経突起の成長円錐などに特徴的に存在しているのが観察された.また,LAPS18cDNAをCOS細胞にtransfectして発現させたところ,LAPS18蛋白質が培養液中に遊離していた.これらの結果は,LAPS18が分泌蛋白として細胞表面において機能していることを示しており,その機能として細胞どうしの接着,神経突起の伸長やシナプス形成などに関与していることが予想された.そこで細胞の移動に関係しているかどうか調べる目的で,初代培養神経の培養液中にLAPS18または抗LAPS18抗体を加えて,細胞の移動を観察した.その結果,LAPS18は細胞の凝集を促進し,抗LAPS18抗体は細胞の移動を抑制した.これらの結果から,LAPS18は神経回路の構造的変化を通じて記憶形成に関わっていることが予想される.

Research Products

(13 results)

All Other

All Publications (13 results)

[Publications] Inoue,T. et al.: "Phase-dependent filtering of sensory information in the oscillating olfactory center of terrestrial mollusk"J.Neurophysiol.. 84. 1112-1115 (2000)
[Publications] Tomita,S. et al.: "PDZ domain-dependent suppression of NF-kB/p65-inducedA_42 production by a neuron specific XII-like protein"J.Biol.Chem.. 275. 13056-13060 (2000)
[Publications] Iijima,K. et al.: "Neuron-specific phosphorylation of Alzheimer's _-amyloid precursor protein by Cdk5"J.Neurochem. 75. 1085-1091 (2000)
[Publications] Lee,D. et al.: "Regulation of XIIL-dependent APP metabolism by XB51, a novel XIIL-binding protein"J.Biol.Chem.. 275. 23134-23138 (2000)
[Publications] Lee,D. et al.: "Rapid transbilaver phospholipid redistribution associated with exocytotic release of neurotransmitters from cholinergic nerve terminals isolated from electric ray Narke japonica"Neurosci.Lett.. 291. 21-24 (2000)
[Publications] Toda,S. et al.: "Image analysis of olfactory responses in the procerebrum of the terrestrial slug Limax marginatus."J.Exp.Biol.. 203. 2895-2905 (2000)
[Publications] Nakaya,T. et al.: "Identification and expression of a novel gene in odor-taste associative learning in the terrestrial slug"Genes Cells. 6. 43-56 (2001)
[Publications] Watanabe,S. et al.: "Modulation of oscillatory neural activities by cholinergic activation of interneurons in the olfactory center of a terrestrial slug"Brain Res.. (in press). (2001)
[Publications] Kishimoto,Y. et al.: "Classical eyeblink conditioning in glutamate receptor subunit d2 mutant mice is impaired in delay paradigm but not in trace paradigm"Eur.J.Neurosci.. (in press). (2001)
[Publications] Kishimoto,Y. et al.: "Long-trace interval eyeblink conditioning is impaired in mutant mice lacking NMDA receptor subunit_1"Eur.J.Neurosci.. (in press). (2001)
[Publications] Shindo,K. et al.: "Solution structure of micelle-bound H5 peptide (427-452) : A primary structure corresponding to the pore-forming region of the voltage dependent potassium channel"Biochim.Biophys.Acta. (in press). (2001)
[Publications] Satoh,Y. et al.: "β-Amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray"Neurosci.Lett.. (in press). (2001)
[Publications] 桐野豊外7名: "生物物理の最前線(印刷中)"講談社.

2000 Fiscal Year Annual Research Report

ナメクジにおける学習特異的蛋白質LAPS18の機能解析と連合学習の分子神経機構

Principal Investigator

桐野 豊 東京大学, 大学院・薬学系研究科, 教授 (10012668)

Research Products

[Publications] Inoue,T. et al.: "Phase-dependent filtering of sensory information in the oscillating olfactory center of terrestrial mollusk"J.Neurophysiol.. 84. 1112-1115 (2000)

[Publications] Tomita,S. et al.: "PDZ domain-dependent suppression of NF-kB/p65-inducedA_42 production by a neuron specific XII-like protein"J.Biol.Chem.. 275. 13056-13060 (2000)

[Publications] Iijima,K. et al.: "Neuron-specific phosphorylation of Alzheimer's _-amyloid precursor protein by Cdk5"J.Neurochem. 75. 1085-1091 (2000)

[Publications] Lee,D. et al.: "Regulation of XIIL-dependent APP metabolism by XB51, a novel XIIL-binding protein"J.Biol.Chem.. 275. 23134-23138 (2000)

[Publications] Lee,D. et al.: "Rapid transbilaver phospholipid redistribution associated with exocytotic release of neurotransmitters from cholinergic nerve terminals isolated from electric ray Narke japonica"Neurosci.Lett.. 291. 21-24 (2000)

[Publications] Toda,S. et al.: "Image analysis of olfactory responses in the procerebrum of the terrestrial slug Limax marginatus."J.Exp.Biol.. 203. 2895-2905 (2000)

[Publications] Nakaya,T. et al.: "Identification and expression of a novel gene in odor-taste associative learning in the terrestrial slug"Genes Cells. 6. 43-56 (2001)

[Publications] Watanabe,S. et al.: "Modulation of oscillatory neural activities by cholinergic activation of interneurons in the olfactory center of a terrestrial slug"Brain Res.. (in press). (2001)

[Publications] Kishimoto,Y. et al.: "Classical eyeblink conditioning in glutamate receptor subunit d2 mutant mice is impaired in delay paradigm but not in trace paradigm"Eur.J.Neurosci.. (in press). (2001)

[Publications] Kishimoto,Y. et al.: "Long-trace interval eyeblink conditioning is impaired in mutant mice lacking NMDA receptor subunit_1"Eur.J.Neurosci.. (in press). (2001)

[Publications] Shindo,K. et al.: "Solution structure of micelle-bound H5 peptide (427-452) : A primary structure corresponding to the pore-forming region of the voltage dependent potassium channel"Biochim.Biophys.Acta. (in press). (2001)

[Publications] Satoh,Y. et al.: "β-Amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray"Neurosci.Lett.. (in press). (2001)

[Publications] 桐野豊 外7名: "生物物理の最前線(印刷中)"講談社.

桐野豊東京大学, 大学院・薬学系研究科, 教授 (10012668)

[Publications] 桐野豊外7名: "生物物理の最前線(印刷中)"講談社.