Identification of tumor-specific antigens recognized by human T cells

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 12213111
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Department of Immunogenetics, Graduate School of Medical Sciences, Kumamoto University
• Principal Investigator: NISHIMURA Yasuharu Kumamoto University, Department of Immunogenetics, Graduate School of Medical Sciences, Professor, 大学院医学薬学研究部, 教授 (10156119)
• Co-Investigator(Kenkyū-buntansha): SENJU Satoru Kumamoto University, Department of Immunogenetics, Graduate School of Medical Sciences, Associate Professor, 大学院医学薬学研究部, 助教授 (50274709) ; NAKATSURA Tetsuya Kumamoto University, Department of Immunogenetics, Graduate School of Medical Sciences, Research Associate, 大学院医学薬学研究部, 助手 (30343354)
• Project Period (FY): 2000 – 2004
• Keywords: Tumor-specific antigens / Tumor markers / Anti-tumor immunity / Cytotoxic T lymphocytes / Antigenic epitopes / cDNA microarray analysis / Serex method / Hepatocellular carcinoma / Melanoma (CONTINUE TO NEXT PAGE)

Research Abstract

The aims of this research project are as follows. 1) to identify tumor-specific antigens strongly expressed in cancer tissues as compared with various normal tissues, 2) to investigate possible usefulness of these tumor antigens for diagnosis and treatment of cancers. In these five years of research period, we identified the following six tumor-specific antigens useful for the cancer diagnosis and/or therapy. 1) Glypican-3 (GPC3); GPC3 is an oncofetal antigen and a GPI-anchored membrane protein. GPC3 is strongly expressed in hepatocellular carcinoma (HCC) and melanoma, but not expressed in various normal adult tissues. The serum soluble GPC3 was detected in about 40% of patients with HCC as well as melanoma. It is worth to note that the patients with even early stage of these cancers were positive for serum GPC3. GPC3 peptide-specific and MHC class I-restricted CTLs could be generated by stimulation with GPC3 peptides of human peripheral blood mononuclear cells (PBMCs) in vitro or stim ulation of mice in vivo. The mice pre-immunized with bone marrow-derived dendritic cells (DCs) pulsed with the GPC3 peptides were protected from the growth of transplanted colon cancer cell line, C26 transfected with mouse GPC3, in a CD8^+ CTL-dependent manner. 2) Proliferation potential related protein (PP-RP); PP-RP is a nuclear protein co-localized with chromosome during mitosis. PP-RP is strongly expressed in esophageal cancer cells and moderate expression was observed in testis and placenta but not in many other normal tissues. Knock down of PP-RP gene expression by RNAi inhibited the cell proliferation of esophageal cancer cell line, and the strong expression of PP-RP correlated with poor prognosis of the patients. Human CTLs could be generated by stimulation of PBMCs with PP-RP-derived peptides and these CTLs killed esophageal cancer cell line both in vitro and in vivo in nude mice transplanted with human cancer cells. 3) KM-HN-1, HSP-105, CLP and KM-PA2 ; these antigens were identified by SEREX method using cancer patients sera and cDNA expression libraries established from cancer cells or testis. These antigens are strongly expressed in several types of cancer cells and in several normal tissues at a lower level. CTLs specific to peptides derived from these antigens were generated and they killed cancer cells in both human and mice. 4) Embryonic stem (ES) cell-derived DC vaccine; we succeeded in augmentation of anti-tumor immunity by immunization of mice with ES cell-derived DCs expressing simultaneously genes encoding for an antigen and chemokines.

Research Products

• [Journal Article] Prevention of experimental autoimmune encephalomyelitis by transfer of EScell-derived dendritic cells expressing MOG peptide along with TRAIL or PD-L1. (2005)
  • Author(s): Hirata, S.et al.
  • Journal Title: J.Immunol. 174 Pages: 1888-1897
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prevention of experimental autoimmune encephalomyelitis by transfer of ES cell-derived dendritic cells expressing MOG peptide along with TRAIL or PD-L1. (2005)
  • Author(s): Hirata, S., Senju, S., Matsuyoshi, H., Fukuma, D., Uemura, Y., Nishimura, Y.
  • Journal Title: J.Immunol. 174 Pages: 1888-1897
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mouse homologue of a novel human oncofetal antigen, Glypican-3, evokes T cell-mediated tumor rejection without autoimmune reactions in mice. (2004)
  • Author(s): Nakatsura, T.et al.
  • Journal Title: Clin.Cancer Res. 10 Pages: 8630-8640
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of a novel human cancer/testis antigen, KM-HN-1, recognizedby cellular and humoral immune responses. (2004)
  • Author(s): Monji, M.et al.
  • Journal Title: Clin.Cancer Res. 10 Pages: 6047-6057
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of glypican-3 as a novel tumor marker for melanoma. (2004)
  • Author(s): Nakatsura, T.et al.
  • Journal Title: Clin.Cancer Res. 10 Pages: 6612-6621
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Proliferation potential-related protein, an ideal esophageal cancer antigen forimmnotherapy, identified using complementary DNA microarray analysis. (2004)
  • Author(s): Yoshitake, Y.et al.
  • Journal Title: Clin.Cancer Res. 10 Pages: 6437-6448
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enhanced priming of antigen-specific CTL in vivo by transfer of ES cell-derived dendritic cells expressing chemokine along with antigenic protein; application to anti-tumor vaccination.anti-tumor vaccination. (2004)
  • Author(s): Matsuyoshi, H.et al.
  • Journal Title: J.Immunol. 172 Pages: 776-786
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] B-Raf contributes to sustained extracellular signal-regulated kinase activationassociated with interleukin-2 production stimulated through the T cell receptor. (2004)
  • Author(s): Tsukamoto, H.et al.
  • Journal Title: J.Biol.Chem. 279 Pages: 48457-48465
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] [Review] Degenerate recognition and response of human CD4^+ Th cell clones : Implications for basic and applied immunology. (2004)
  • Author(s): Nishimura, Y.et al.
  • Journal Title: Mol.Immunol. 40 Pages: 1089-1094
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves' disease. (2004)
  • Author(s): Ohkura, T.et al.
  • Journal Title: Biochem.Biophys.Res.Comm 321 Pages: 432-440
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparison of frequency of interferon-gamma-positive CD4^+ T cells before and after percutaneous coronary intervention and the effect of statin therapy in patients with stable angina pectoris. (2004)
  • Author(s): Tanaka, T.et al.
  • Journal Title: Am.J.Cardiol. 93 Pages: 1547-1549
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 抗原とケモカインを共発現させた ES 細胞由来の樹状細胞を用いた抗腫瘍免疫療法 (2004)
  • Author(s): 松吉秀武, 千住 覚, 西村泰治
  • Journal Title: 臨床免疫 42(1) Pages: 110-114
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 腫瘍抗原ペプチドを駆使した癌免疫療法の新展開 (2004)
  • Author(s): 中面哲也, 西村泰治
  • Journal Title: 実験医学 22(5) Pages: 215-220
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] cDNA マイクロアレイ解析による腫瘍特異抗原の探索 (2004)
  • Author(s): 中面哲也, 西村泰治
  • Journal Title: 臨床免疫 41(4) Pages: 369-378
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] cDNA マイクロアレイ解析による腫瘍特異抗原の探索「肝細胞癌の腫瘍マーカーおよび腫瘍特異抗原としてのGlypican-3(GPC3)の同定;その臨床応用への可能性 (2004)
  • Author(s): 中面哲也, 西村泰治
  • Journal Title: 医学をゲノムする(第27回阿蘇シンポジウム記録2003) Pages: 115-127
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mouse homologue of a novel human oncofetal antigen, Glypican-3, evokes T cell-mediated tumor rejection without autoimmune reactions in mice. (2004)
  • Author(s): Nakatsura, T., Komori, H., Kubo, T., Yoshitake, Y., Senju, S., Katagiri, T., Furukawa, Y., Oagawa, M., Nakamura, Y., Nishimura, Y.
  • Journal Title: Clin.Cancer Res. 10 Pages: 8630-8640
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of a novel human cancer/testis antigen, KM-HN-1, recognized by cellular and humoral immune responses. (2004)
  • Author(s): Monji, M., Nakatsura, T., Senju, S., Yoshitake, Y., Sawatsubashi, M., Shinohara, M., Kageshita, T., Ono, T., Inokuchi, A., Nishimura, Y.
  • Journal Title: Clin.Cancer Res. 10 Pages: 6047-6057
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of glypican-3 tumor marker for melanoma. (2004)
  • Author(s): Nakatsura, T., Kageshita, T., Ito, S., Wakamatsu, K., Monji, M., Senju, S., Ono, T., Nishimura, Y.
  • Journal Title: Clin.Cancer Res. 10 Pages: 6612-6621
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Proliferation potential-related protein, an ideal esophageal cancer antigen for immnotherapy, identified using complementary DNA microarray analysis. (2004)
  • Author(s): Yoshitake, Y., Nakatsura, T., Monji, M., Senju, S., Matsuyoshi, H., Tsukamoto, H., Hosaka, S., Komori, H., Fukuma, D., Ikuta, Y., Katagiri, T., Furukawa, Y., Ito, H., Shinohara, M., Nakamura, Y., Nishimura, Y.
  • Journal Title: Clin.Cancer Res. 10 Pages: 6437-6448
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhanced priming of antigen-specific CTLs in vivo by embryonic stem cell-derived dendritic cells expressing chemokine along with antigenic protein; application to antitumor vaccination. (2004)
  • Author(s): Matsuyoshi, H., Senju, S., Hirata, S., Yoshitake, Y., Uemura, Y., Nishimura, Y.
  • Journal Title: J.Immunol. 172 Pages: 776-786
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] B-Raf contributes to sustained extracellular signal-regulated kinase activation associated with interleukin-2 production stimulated through the T cell receptor. (2004)
  • Author(s): Tsukamoto, H., Irie, A., Nishimura, Y.
  • Journal Title: J.Biol.Chem. 279 Pages: 48457-48465
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Review, Degenerate recognition and response of human CD4^+ Th cell clones : implications for basic and applied immunology. (2004)
  • Author(s): Nishimura, Y., Chen, Y-Z., Uemura, Y., Tanaka, Y., Tsukamoto, H., Kanai, T., Yokomizo, H., Yun, C., Matsuoka, T., Irie, A., Matsushita, S.
  • Journal Title: Mol.Immunol. 40 Pages: 1089-1094
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves' disease. (2004)
  • Author(s): Ohkura, T., Taniguchi, S., Yamada, K., Nishio, N., Okamura, T., Yoshida, A., Kamijou, K., Fukata, S., Kuma, K., Inoue, Y., Hisatome, I., Senju, S., Nishimura Y., Shigemasa, C.
  • Journal Title: Biochem.Biophys.Res.Comm 321 Pages: 432-440
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparison of frequency of interferon-gamma- positive CD4^+ T cells before and after percutaneous coronary intervention and the effect of statin therapy in patients with stable angina pectoris. (2004)
  • Author(s): Tanaka T., Soejima, H., Hirai, N., Sakamoto, T., Yoshimura, M., Kaji, I., Miyao Y., Fujimoto, K., Miyagi, H., Irie, A., Nishimura, Y., Ogawa, H.
  • Journal Title: Am.J.Cardiol. 93 Pages: 1547-1549
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Generation and genetic modification of dendritic cells derived from mouse embryonic stem cells. (2003)
  • Author(s): Senju, S.et al.
  • Journal Title: Blood 101 Pages: 3501-3508
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Systematic analysis of the combinatorial nature of epitopes recognized by TCR leads to identification of mimicry epitopes for GAD65 specific TCRs (2003)
  • Author(s): Uemura, Y.et al.
  • Journal Title: J.Immunol. 170 Pages: 947-960
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Unique T cell proliferation dependent on PKCm activation with impaired Zap-70 phosphorylation in recognition of over-expressed HLA-DR/partially agonistic peptide complexes. (2003)
  • Author(s): Irie, A.et al.
  • Journal Title: Eur.J.Immunol. 33 Pages: 1497-1507
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The preference to a Th1-type response in patients with coronary spastic angina. (2003)
  • Author(s): Soejima, H.et al.
  • Journal Title: Circulation 107 Pages: 2196-2200
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cellular vaccination with genetically modified dendritic cells derived from mouse ES cells. (2003)
  • Author(s): Senju, S.et al.
  • Journal Title: J.Invest.Dermatol 121 Pages: 1241
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Glypican-3, overexpressed specifically in human hepatocellular carcinoma, is a novel tumor marker (2003)
  • Author(s): Nakatsura, T.et al.
  • Journal Title: Biochem.Biophys.Res.Comm 306 Pages: 16-25
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of naturally processed helper T-cell epitopes from prostate-specific membrane antigen using peptide-based in vitro stimulation. (2003)
  • Author(s): Kobayashi, H.et al.
  • Journal Title: Clin. Cancer Res. 9 Pages: 5386-5393
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Heat shock protein 105 is overexpressed in a variety of human tumors. (2003)
  • Author(s): Kai, M.et al.
  • Journal Title: Oncology reports 10 Pages: 1777-1782
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] [Review] Specificity, degeneracy, and molecular mimicry in antigen recognition by HLA-class II restricted T cell receptors; implications for clinical medicine. (2003)
  • Author(s): Uemura, Y.et al.
  • Journal Title: Modern Rheumatology 10 Pages: 205-214
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 癌とHLA-癌細胞の排除におけるHLAの役割 (2003)
  • Author(s): 中面哲也, 西村泰治
  • Journal Title: 医学のあゆみ 207(8) Pages: 543-548
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Generation and genetic modification of dendritic cells derived from mouse embryonic stem cells. (2003)
  • Author(s): Senju, S., Hirata, S., Matsuyoshi, H., Masuda, M., Uemura, Y., Araki, K., Yamamura, K.I., Nishimura, Y.
  • Journal Title: Blood 101 Pages: 3501-3508
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Systematic analysis of the combinatorial nature of epitopes recognized by TCR leads to identification of mimicry epitopes for GAD65 specific TCRs. (2003)
  • Author(s): Uemura, Y., Senju, S., Maenaka, K., Iwai, L.K., Fujii, S., Tabata, H., Tsukamoto, H., Hirata, S., Chen, Y-Z, Nishimura, Y.
  • Journal Title: J.Immunol. 170 Pages: 947-960
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Unique T cell proliferation associated with PKCm activation and impaired Zap-70 phosphorylation in recognition of overexpressed HLA/partially agonistic peptide complexes. (2003)
  • Author(s): Irie, A., Chen Y-Z, Tsukamoto, H., Jotsuka, T., Masuda, M., Nishimura, Y.
  • Journal Title: Eur.J.Immunol. 33 Pages: 1497-1507
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Preference towards a Th1-type response in patients with coronary spastic angina. (2003)
  • Author(s): Soejima, H., Irie, A., Miyamoto, S., Kajiwara, I., Kojima, S., Hokamaki, J., Sakamoto, T., Tanaka, T., Yoshimura, M., Nishimura, Y., Ogawa, H.
  • Journal Title: Circulation 107 Pages: 2196-2200
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cellular vaccination with genetically modified dendritic cells derived from mouse ES cells. (2003)
  • Author(s): Senju S, Matsuyoshi H, Hirata S, Nishimura Y.
  • Journal Title: J.Invest.Dermatol. 121 Pages: 1241
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Glypican-3, overexpressed specifically in human hepatocellular carcinoma, is a novel tumor marker. (2003)
  • Author(s): Nakatsura, T., Yoshitake, Y., Senju, S., Monji, M., Komori, H., Motomura, Y., Hosaka, S., Beppu, T., Ishiko, T., Kamohara, H., Ashihara, H., Katagiri, T., Furukawa, Y., Fujiyama, S., Ogawa, M., Nakamura, Y., Nishimura, Y.
  • Journal Title: Biochem.Biophys.Res.Comm. 306 Pages: 16-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of naturally processed helper T-cell epitopes from prostate-specific membrane antigen using peptide-based in vitro stimulation. (2003)
  • Author(s): Kobayashi, H., Omiya, R., Sodey, B., Yanai, M., Oikawa, K., Sato, K., Kimura, S., Senju, S., Nishimura, Y., Tateno, M., Celis, E.
  • Journal Title: Clin.CancerRes. 9 Pages: 5386-5393
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Heat shock protein 105 is overexpressed in a variety of human tumors. (2003)
  • Author(s): Kai, M., Nakatsura, T., Egami, H., Senju, S., Nishimura, Y., Ogawa, M.
  • Journal Title: Oncology reports 10 Pages: 1777-82
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] [Review] Specificity, degeneracy, and molecular mimicry in antigen recognition by HLA-class II restricted T cell receptors; implications for clinical medicine. (2003)
  • Author(s): Uemura, Y., Senju, S., Fujii, S., Iwai, L.K., Maenaka, K., Tabata, H., Kanai, T., Chen, Y-Z., Nishimura, Y.
  • Journal Title: Modern Rheumatology 13 Pages: 205-214
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cellular and humoral immune responses to a human pancreatic cancer antigen, CLP, originally defined by the SEREX method (2002)
  • Author(s): Nakatsura, T.et al.
  • Journal Title: Eur.J.Immunol. 32 Pages: 826-836
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Head and neck cancer antigens recognized by the humoral immune system (2002)
  • Author(s): Monji, M. et al.
  • Journal Title: Biochem.Biophys.Res.Comm. 294 Pages: 734-741
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and immunocytochemical analysis of DCNP1, a dendritic cell-associated nuclear protein (2002)
  • Author(s): Masuda, M.et al.
  • Journal Title: Biochem.Biophys.Res.Comm. 290 Pages: 1022-1029
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cross-linking HLA-DR molecules on Th1 cells induces anaergy in association with increased level of cyclin-dependent kinase inhibitor p27Kip1 (2002)
  • Author(s): Kudo, H.et al.
  • Journal Title: Immunol.Lts. 81 Pages: 149-155
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 「SEREX」第34回臨床免疫の近未来 (2002)
  • Author(s): 中面哲也, 門司幹男, 西村泰治
  • Journal Title: Surgery Frontier 9(3) Pages: 58-61
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 樹状細胞による抗原のプロセシングと提示 (2002)
  • Author(s): 千住 覚, 西村泰治
  • Journal Title: 医学のあゆみ 200(6) Pages: 467-471
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cellular and humoral immune responses to a human pancreatic cancer antigen, CLP, originally defined by the SEREX method. (2002)
  • Author(s): Nakatsura, T., Senju, S., Ito, M., Nishimura, Y.*, Itoh, K.*(^*equal contribution)
  • Journal Title: Eur.J.Immunol. 32 Pages: 826-836
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Head and neck cancer antigens recognized by the humoral immune system. (2002)
  • Author(s): Monji, M., Senju, S., Nakatsura, T., Yamada, K., Sawatsubashi, M., Inokuchi, A., Nishimura, Y.
  • Journal Title: Biochem.Biophys.Res.Comm. 294 Pages: 734-741
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification and immunocytochemical analysis of DCNP1, a dendritic cell-associated nuclear protein. (2002)
  • Author(s): Masuda, M., Senju, S., Fujii, S-I., Terasaki, Y., Takeya, M., Hashimoto, S-I., Matsushima, K., Yumoto, E., Nishimura, Y.
  • Journal Title: Biochem.Biophys.Res.Comm. 290 Pages: 1022-1029
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cross-linking HLA-DR molecules on Th1 cells induces anaergy in association with increased level of cyclin- dependent kinase inhibitor p27Kip1. (2002)
  • Author(s): Kudo, H., Matsuoka, T., Mitsuya, H., Nishimura Y., Matsushita, S.
  • Journal Title: Immunol.Lts. 81 Pages: 149-155
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Lens epitherial derived growth factor as an autoantigen in Vogt-Koyanagi-Harada disease. (2001)
  • Author(s): Yamada, K.et al.
  • Journal Title: Immunol. Lts. 78 Pages: 161-168
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Establishment of an expression cloning system for CD4^+ T cell epitopes (2001)
  • Author(s): Fujii, S.et al.
  • Journal Title: Biochem.Biophys.Res.Comm. 284 Pages: 1140-1147
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Gene cloning of immunogenic antigens over-expressed in pancreatic cancer (2001)
  • Author(s): Nakatsura, T.et al.
  • Journal Title: Biochem.Biophys.Res.Comm. 281 Pages: 936-944
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of human antitumor cytotoxic T lymphocytes epitopes of recoverin, cancer-associated retinopathy antigen, to achieve a clinical better prognosis in a paraneoplastic syndrome. (2001)
  • Author(s): Maeda, A.et al.
  • Journal Title: Eur.J.Immunol. 31 Pages: 563-572
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differences between T cell reactivities to major myelin protein-derived peptides in opticospinal and conventional forms of multiple sclerosis and healthy controls. (2001)
  • Author(s): Minohara, M.et al.
  • Journal Title: Tissue Antigens 57 Pages: 447-456
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Combination of HLA-A and HLA class II alleles controls the susceptibility to rheumatoid arthritis. (2001)
  • Author(s): Tsuchiya K.et al.
  • Journal Title: Tissue Antigens 58 Pages: 395-401
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of a novel autoantigen UACA in patients with panuveitis. (2001)
  • Author(s): Yamada, K.et al.
  • Journal Title: Biochem.Biophys.Res.Comm. 280 Pages: 1169-1176
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of β-lactoglobulin-derived peptides and class II HLA molecules recognized by T Cells from patients with milk allergy. (2001)
  • Author(s): Inoue, R.et al.
  • Journal Title: Clin.Exp.Immunol 31 Pages: 1126-1134
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] (review) Molecular and cellular analyses of HLA class II -associated susceptibility to autoimmune diseases in the Japanese population (2001)
  • Author(s): Nishimura, Y.et al.
  • Journal Title: Modern Rheumatology 11 Pages: 103-112
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 「MHCによるTリンパ球への抗原提示」,特集 : 免疫学研究の最新動向 (2001)
  • Author(s): 西村泰治
  • Journal Title: 学術月報 54(7) Pages: 685-689
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Lens epitherial derived growth factor as an autoantigen in Vogt- Koyanagi-Harada disease. (2001)
  • Author(s): Yamada, K., Senju, S., Shinohara, T., Nakatsura, T., Murata, Y., Ishihara, M., Nakamura, S., Ohno, S., Negi, A., Nishimura, Y.
  • Journal Title: Immunol.Lts. 78 Pages: 161-168
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Establishment of an expression cloning system for CD4^+ T cell epitopes. (2001)
  • Author(s): Fujii, S.*, Uemura, Y.*, Iwai, L.K., Ando, M., Senju, S., Nishimura, Y.(^*equal contribution)
  • Journal Title: Biochem.Biophys.Res.Comm. 284 Pages: 1140-1147
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gene cloning of immunogenic antigens over- expressed in pancreatic cancer. (2001)
  • Author(s): Nakatsura, T., Senju, S., Yamada, K., Jyotsuka, T., Ogawa, M., Nishimura, Y.
  • Journal Title: Biochem.Biophys.Res.Comm. 281 Pages: 936-944
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of human antitumor cytotoxic T lymphocytes epitopes of recoverin, cancer-associated retinopathy antigen, to achieve a clinical better prognosis in a paraneoplastic syndrome. (2001)
  • Author(s): Maeda, A., Ohguro, H., Nabeta, Y., Hirohashi, Y., Sahara, H., Maeda, T., Wada, Y., Sato, T., Yun, C., Nishimura, Y., Kuroki, Y., Sato, N.
  • Journal Title: Eur.J.Immunol 31 Pages: 563-572
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differences between T cell reactivities to major myelin protein-derived peptides in opticospinal and conventional forms of multiple sclerosis and healthy controls. (2001)
  • Author(s): Minohara, M., Ochi, H., Matsushita, S., Irie, A., Nishimura, Y., Kira, J-I.
  • Journal Title: Tissue Antigens 57 Pages: 447-456
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Combination of HLA-A and HLA class II alleles controls the susceptibility to rheumatoid arthritis. (2001)
  • Author(s): Tsuchiya K, Kimura A, Kondo M, Nishimura Y, Sasazuki T
  • Journal Title: Tissue Antigens 58 Pages: 395-401
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of a novel autoantigen UACA in patients withpanuveitis. (2001)
  • Author(s): Yamada, K., Senju, S., Nakatsura, T., Murata, Y., Ishihara, M., Nakamura, S., Ohno, S., Negi, A., Nishimura, Y.
  • Journal Title: Biochem.Biophys.Res.Comm. 280 Pages: 1169-1176
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of β-lactoglobulin- derived peptides and class II HLA molecules recognized by T Cells from patients with milk allergy. (2001)
  • Author(s): Inoue, R., Matsushita, S., Kaneko, H., Shinoda, S., Sakaguchi, H., Nishimura, Y., Kondo, N.
  • Journal Title: Clin.Exp.Immunol. 31 Pages: 1126-1134
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] (review) Molecular and cellular analyses of HLA class II-associated susceptibility to autoimmune diseases in the Japanese population. (2001)
  • Author(s): Nishimura, Y., Ito, H., Tabata, H., Fujii, S., Tokano, Y., Chen, Y-Z., Matsuda, I., Mitsuya, H., Kira, J-I., Hashimoto, H., Senju, S., Matsushita, S.
  • Journal Title: Modern Rheumatology 11 Pages: 103-112
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immunocytochemical analyses and targeted gene disruption of GTPBP1. (2000)
  • Author(s): Senju, S.et al.
  • Journal Title: Mol.Cell Biol. 20 Pages: 6195-6200
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ligation of HLA-DR molecules on B cells induces enhanced expression of IgM heavy chain genes in association with Syk activation. (2000)
  • Author(s): Tabata, H.et al.
  • Journal Title: J.Biol.Chem. 275 Pages: 34998-35005
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Na^+-dependence of early signaling in human T cell activation : role of voltage-gated Na^+ channels. (2000)
  • Author(s): Lai, Z-F.et al.
  • Journal Title: J.Immunol. 165 Pages: 83-90
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Fine specificity of T cells reactive to human PDC-E2 163-176 peptide, the immunodominant autoantigen in primary biliary cirrhosis : implications for molecular mimicry and cross-recognition among mitochondrial autoantigens. (2000)
  • Author(s): Shigematsu, H.et al.
  • Journal Title: Hepatology 32 Pages: 901-909
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of mouse and human GTPBP2, new members of GP-1 family of GTPase. (2000)
  • Author(s): Kudoh, H.et al.
  • Journal Title: Biochem.Biophys.Res.Comm. 272 Pages: 456-465
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Both the HLA-DPB1 and -DRB1 alleles correlate with risk for multiple sclerosis in Japanese : Clinical phenotypes and gender as important factors. (2000)
  • Author(s): Fukazawa, T.et al.
  • Journal Title: Tissue Antigens 55 Pages: 199-205
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analysis of T-cell responses to the β2-glycoprotein I-derived peptide libraly in patients with anti-β2-glycoprotein I antibody-associated autoimmunity. (2000)
  • Author(s): Ito, H.et al.
  • Journal Title: Human Immunol. 61 Pages: 366-377
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analysis of HLA-DRB1 alleles in Japanese patients with chronic myelogenous leukemia. (2000)
  • Author(s): Yasukawa, M.et al.
  • Journal Title: Am.J.Hematol. 63 Pages: 99-101
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Multicenter cooperative study of HLA class II alleles in Japanese patients with systemic lupus erythematosus. (2000)
  • Author(s): Morimoto, S.et al.
  • Journal Title: Mod. Rheumatol. 10 Pages: 235-239
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] CLIP置換型インバリアント鎖遺伝子を利用した HLA クラスII・ペプチド複合体発現細胞ライブラリーの構築:がん免疫療法への応用の可能性 (2000)
  • Author(s): 藤井慎嗣, 西村泰治
  • Journal Title: 分子細胞治療,特集:がん免疫療法の最前線 1(1) Pages: 14-22
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] T 細胞に抗原を認識させる主要組織適合抗原の構造と機能 (2000)
  • Author(s): 西村泰治
  • Journal Title: 蛋白質・核酸・酵素 45(7) Pages: 1205-1218
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immunocytochemical analyses and targeted gene disruption of GTPBP1. (2000)
  • Author(s): Senju, S., Iyama, K-I, Kudo, H., Aizawa, S., Nishimura, Y.
  • Journal Title: Mol.Cell Biol. 20 Pages: 6195-6200
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ligation of HLA-DR molecules on B cells induces enhanced expression of IgM heavy chain genes in association with Syk activation. (2000)
  • Author(s): Tabata, H., Matsuoka, T., Endo, R, Nishimura, Y., Matsushita, S.
  • Journal Title: J.Biol.Chem. 275 Pages: 34998-35005
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Na^+- dependence of early signaling in human T cell activation: role of voltage-gated Na^+ channels. (2000)
  • Author(s): Lai, Z-R, Chen, Y-Z., Nishimura, Y, Nishi, K.
  • Journal Title: J.Immunol. 165 Pages: 83-90
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Fine specificity of T cells reactive to human PDC-E2 163-176 peptide, the immunodominant autoantigen in primary biliary cirrhosis: implications for molecular mimicry and cross-recognition among mitochondrial autoantigens. (2000)
  • Author(s): Shigematsu, H., Shimoda, S., Nakamura, M., Matsushita, S., Nishimura, Y, Sakamoto, N., Ichiki, Y, Niho, Y, Gershwin, M.E., Ishibashi, H.
  • Journal Title: Hepatology 32 Pages: 901-909
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of mouse and human GTPBP2, new members of GP- 1 family of GTPase. (2000)
  • Author(s): Kudoh, H., Senju, S., Mitsuya, H., Nishimura, Y.
  • Journal Title: Biochem.Biophys.Res.Comm. 272 Pages: 456-465
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Both the HLA-DPB1 and-DRB1 alleles correlate with risk for multiple sclerosis in Japanese : Clinical phenotypes and gender as important factors. (2000)
  • Author(s): Fukazawa, T., Yamasaki, K., Ito, H., Kikuchi, S., Minohara, M., Horiuchi, I., Tsukishima, E., Sasaki, H., Hamada, T., Nishimura, Y., Tashiro, K., Kira, J.
  • Journal Title: Tissue Antigens 55 Pages: 199-205
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Analysis of T-cell responses to the β2-glycoprotein I-derived peptide libraly in patients with anti-β2-glycoprotein I antibody-associated autoimmunity. (2000)
  • Author(s): Ito, H., Matsushita, S., Tokano, Y., Nishimura, H., Tanaka, Y., Fujisao, S., Mitsuya, H., Hashimoto, H., Nishimura, Y.
  • Journal Title: Human Immunol. 61 Pages: 366-377
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Analysis of HLA-DRB1 alleles in Japanese patients with chronic myelogenous leukemia. (2000)
  • Author(s): Yasukawa, M., Ohminami, H., Kojima, K., Inokuchi, K., Nishimura, Y., Fujita, S.
  • Journal Title: Am.J.Hematol. 63 Pages: 99-101
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Multicenter cooperative study of HLA class II alleles in Japanese patients with systemic lupus erythematosus. (2000)
  • Author(s): Morimoto, S., Hashimoto, H., Yamanaka, K., Tokano, Y., Nishimura, Y., Sawada, S., Kamatani, N., Sumida, T., Yoshizaki, K., Nagasawa, K., Miyasaka, N.
  • Journal Title: Mod.Rheumatol. 10 Pages: 235-239
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 新版・臨床免疫学 (2001)
  • Author(s): 西村泰治, 千住 覚
  • Total Pages: 55-62
  • Publisher: 講談社(東京)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 現代医学の基礎,第11巻,感染と生体防御 (2000)
  • Author(s): 西村泰治
  • Total Pages: 221-243
  • Publisher: 岩波書店(東京)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] わかる実験医学シリーズ : 免疫がわかる (2000)
  • Author(s): 西村泰治
  • Total Pages: 44-55
  • Publisher: 羊土社(東京)
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 悪性黒色腫の新規な診断剤 (2004)
  • Inventor(s): 西村 泰治, 中面 哲也, 生田 義明
  • Industrial Property Rights Holder: 国立大学法人熊本大学
  • Industrial Property Number: 2004-303688
  • Filing Date: 2004-10-19
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] RNAiを利用した抗腫瘍剤 (2004)
  • Inventor(s): 西村 泰治, 中面 哲也, 保坂 征司
  • Industrial Property Rights Holder: 国立大学法人熊本大学
  • Industrial Property Number: 2004-235087
  • Filing Date: 2004-08-12
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 霊長類動物胚性幹細胞から樹状細胞の製造方法 (2004)
  • Inventor(s): 千住 覚, 西村泰治
  • Industrial Property Rights Holder: 千住 覚, 田辺製薬株式会社
  • Industrial Property Number: 2004-249062
  • Filing Date: 2004-08-27
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 食道癌の抗原およびその利用 (2004)
  • Inventor(s): 中面 哲也, 吉武 義泰, 中村 祐輔, 西村 泰治
  • Industrial Property Rights Holder: 熊本TLO
  • Industrial Property Number: 2003-291073
  • Filing Date: 2004-08-27
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 悪性黒色腫(メラノーマ)の診断剤 (2003)
  • Inventor(s): 西村 泰治, 中面 哲也
  • Industrial Property Rights Holder: くまもとTLO
  • Industrial Property Number: 2003-368725
  • Filing Date: 2003-10-29
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 哺乳動物のES細胞由来樹状細胞の産生方法 (2003)
  • Inventor(s): 千住 覚, 西村 泰治
  • Industrial Property Rights Holder: 千住 覚, 西村 泰治, 熊本TLO
  • Industrial Property Number: 2003-108677
  • Filing Date: 2003-04-14
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 頭頚部癌の抗原 (2002)
  • Inventor(s): 西村 泰治, 門司 幹男
  • Industrial Property Rights Holder: 西村 泰治, 門司 幹男, 熊本大学TLO
  • Industrial Property Number: 2002-298833
  • Filing Date: 2002-10-11
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] ペプチド及びこれを含む医薬 (2002)
  • Inventor(s): 西村 泰治, 中面 哲也, 中村 祐輔
  • Industrial Property Rights Holder: キリンビール株式会社
  • Industrial Property Number: 2002-245831
  • Filing Date: 2002-08-26
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 膵癌・大腸癌の抗原、抗原をコードするDNA、ペプチド、蛋白、概抗原により誘導される抗体、キラーT細胞、およびその用途 (2002)
  • Inventor(s): 西村 泰治, 中面 哲也
  • Industrial Property Rights Holder: 西村 泰治, 中面 哲也, 熊本大学TLO
  • Industrial Property Number: 2002-255668
  • Filing Date: 2002-08-30
  • Description: 「研究成果報告書概要(和文)」より