2004 Fiscal Year Final Research Report Summary
Production of cells that constitute microenvironment of cancer.
| Project/Area Number |
12219209
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The Institute of Physical and Chemical Research (2003-2004)
Kyoto University (2000-2002) |
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Principal Investigator |
NISHIKAWA Shun-Ichi The institute of Physical and Chemical Research, Laboratory for Stem Cell Biology, Group Director, 幹細胞研究グループ, グループディレクター (60127115)
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| Co-Investigator(Kenkyū-buntansha) |
ERA Takumi The Institute of Physical and Chemical Research, Laboratory for Stem Cell Biology, Research Scientist, 幹細胞研究グループ, 研究員 (00273706)
OSAWA Masatake The lnstitute of Physical and Chemical Research, Laboratory for Stem Cell Biology, Research Scientist, 幹細胞研究グループ, 研究員 (10344029)
UEMURA Akiyoshi The Institute of Physical and Chemical Research, Laboratory for Stem Cell Biology, Research Scientist, 幹細胞研究グループ, 研究員 (30373278)
TOGAWA Atsushi The lnstitute of Physical and Chemical Research, Laboratory for Stem Cell Biology, Research Scientist, 幹細胞研究グループ, 研究員 (30359799)
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| Project Period (FY) |
2000 – 2004
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| Keywords | ES cell / endothelial cell / stem cell / mesoderm / melanocyte / bulge region / mesenchymal stem cell / DNA microarray |
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| Research Abstract |
1) We established a method to induce vascular components from ES cells that enables cellular analysis of normal endothelial and mural cells. Using this experimental system, we determine the role of Fik1, Fit1, and Flt4 in endothelial cells. Moreover, we have identified a culture condition to induce endothelial cells from human ES cells.
2) We established a method to manipulate angiogenesis in the neonatal retina by intraocular injection of various reagents. Using this system, we have identified LIF and Tlx as molecules regulating astrocyte-endothelial intereactions, and PDGFRβ, Ang1, and Plexin D1 as molecules involved in interaction between endothelial cells and mural cells.
3) Using a culture system to induce ES cell differentiation to hematopoietic stem cells, we identify the stage when SCL, Runx1 and GATA1 are involved. While we succeeded to determine a defined culture condition to induce endothelial and hematopoietic cells from ES cells, we could not attain the goal to induce the de
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finitive hematopoietic stem cells from ES cells.
4) We have established a defined condition that allows selective induction of the definitive endoderm and demonstrated presence of bipotent mesendoderm that can give rise to both definitive endoderm and mesoderm.
5) Taking advantage of a data base that contains DNA microarray data of varying intermediate stages appearing in ES cell differentiation culture (this data base is a project of Kobe City), we tested a new method to isolate novel genes that are involved in various process of cell specification during embryogenesis. We have identified three novel molecules and proved that they are indeed play an essential role in embryogenesis.
6) We determined that the stem cell compartment of melanocyte system locates in the bulge-region of hair follicles. Those melanocyte stem cells are picked up individually to make a stem cell specific library From the gene expression profile in this library, we found that the stem cell compartment is characterized by its low expression of house keeping genes. We also found that the stem cell expresses a high level of 1) Wnt inhibitors, 2) Notch/Hes1, and 3) molecules that may potentially suppress basic transcriptional machinery. Among those characteristics, we investigated the role of Notch signaling in melanocyte, and demonstrated that Notch/Hes1 signaling is essential for melanocyte development and maintenance of the stem cell compartment.
7) We demonstrated that a part of mesenchymal stem cells are derived from neuroepithelial cells. Less
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[Journal Article] Molecular basis for hematopoietic/mesenchymal interaction during initiation of Peyer' s patch organogenesis.2001
Author(s)
Honda K, Nakano H, Yoshida H, Nishikawa S, Rennert P, J Ikuta K, Tamechika M, Yamaguchi K, Fukumoto T, Chiba T, Nishikawa SI
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Journal Title
J. Exp. Med. 195
Pages: 621-630
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Molecular basis for hematopoietic/mesenchymal interaction during initiation of Peyer's patch organogenesis.2001
Author(s)
Honda K, Nakano H, Yoshida H, Nishikawa S, Rennert P, Ikuta K, Tamechika M, Yamaguchi K, Fukumoto T, Chiba T, Nishikawa SI.
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Journal Title
J Exp Med. 193
Pages: 621-630
Description
「研究成果報告書概要(欧文)」より
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