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2001 Fiscal Year Final Research Report Summary

Quantitative microbiological technology for advanced management for water environment

Research Project

Project/Area Number 12305032
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionThe University of Tokyo

Principal Investigator

OHGAKI Shinichiro  Graduate School of Engineering, The University of Tokyo, Professor, 大学院・工学系研究科, 教授 (20005549)

Co-Investigator(Kenkyū-buntansha) HARADA Hideaki  Nagaoka University of Technology, Graduate School oi engineering, Professor, 大学院・工学研究科, 教授 (70134971)
FUJITA Masanori  Osaka University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (70029289)
OMURA Tatsuo  Tohoku University, Graduate School of Engineering, Professor, 大学院・工学系研究科, 教授 (30111248)
ENDO Ginro  Tohoku Gakuin University, Faculty of Engineering, Professor, 工学部, 教授 (80194033)
TSUNO Hiroshi  Kyoto University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (40026315)
Project Period (FY) 2000 – 2001
KeywordsWater microbiology / quantification / gene technology / PCR / FISH / antibiotics / VIRUS / concentration
Research Abstract

There had been a great progress in genetic engineering and analytical methods for water chemistry in the last decade of 20th century. However, these techniques were not directly applied to the field of the environmental engineering, because it includes such difficulty in understanding the phenomena that low concentration and complicated microbial community while many other compounds are interfere the analytical methods. In this study, in order to utilize the emerging technology for environmental engineering for water management, various efforts were paid for quantification of microbes for the purpose of elucidation of complex microbial community and the fates of pathogenic microbes in water environment.

  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] katayama H., Shimasaki A., Ohgaki S.: "Development of a Virus Concentration Method and Its Application to Detection of Enterovirus and Norwalk Virus from Coastal Sea Water"Applied and Environmental Microbiology. 68:3. 1033-1039 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katayama H., M.Nakamura, S.Ohgaki: "PCR determination of inactivated RNA coliphage Qβ"Advances in Water and Wastewater Treatment Technology …Molecular technology, nutrient removal. sludge reduction, and environmental health. 1. 101-108 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sei K., Nakao M., Mori K., Ike M., Kohno T., Fujita M: "Design of PCR primers and a gene probe for extensive detection of poly(3-hydroxybutyrate)(PHB)-degrading bacteria possessing fibronectin type III linker type-PHB depolymerases"Applied Microbiology and Biotechnology. 55. 801-806 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.Sano, K.Fukushi, K.Yano.Y.Yoshida, T.Omura: "Enhanced Virus Recovery from Municipal Sewage Sludge with a Combination of Enzyme and Cation Exchange Resin"Water. Science and Technology. 43(2). 75-82 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Watanabe, D.Sano, T.Omura: "Risk Evaluation for Pathogenic Bacteria and Viruses in the Compost of Sewage Sludge"Proceedings of 1st International Water Association(IWA)Asia-Pacific Regional Conference. 1. 729-734 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Sekiguchi, H.Takahashi, Y.Kamagata, A.Ohashi, H.Harada: "In situ Detection, Isolation and Physiological Properties of a Thin Filamentous Microoganism Abundant in Methanogenic Granular Sludges : a Novel Isolate affiliated with a Clone Cluster, the Green Non Sulfur Bacteria Subdivision 1"Appl. Environ. Microbiol.. 67・12. 5740-5749 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Alam, M. Z. B., Ohtaki, M., Furumai, H., Ohgaki, S.: "Direct and indirect inactivation of Microcystis aeruginosa by UV-radiation"Water Research. Vol. 35, No. 4. 1008-1014 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katayama, H., Shimasaki, A., Ohgaki, S.: "Development of a Virus Concentration Method and Its Application to Detection of Enterovirus and Norwalk Virus from Coastal Seawater"Applied and Environmental Microbiology. 1033-1039 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katayama, H., Kuyama, T., Ohgaki, S.: "Daily and Seasonal Profits of Coliforms and F-specific RNA Phase in Indigenous Oysters and Marine Water in Tokyo Bay"Proc. of 1st IWA Asia-Pacific Regional Conference, Fukuoka. 12-15 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Alam, M.Z.B., Luxmy, B.S., Katayama, H., Ohgaki, S.: "Detecting the Viability of Particle Associated Bacteria after UV-radiation: A Molecular Biological Approach"Proc. of First Asian Pacific Regional Conference of IWA, Fukuoka. 661-666 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei, K., Asano, K., Tateishi, N., Mori, K., Ike, M., Kohno, T., Fujita, M.: "Development of Simple Methods of DNA Extraction from Environmental Sample for Monitoring Microbial Community Based on PCR"Japanese Jounal of Water Treatment Biology. Vol. 36, No. 4. 193-204 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sei, N., Nakano, M., Mori, K., Ike, K., Kohno, T., Fujita, M.: "Design of FOR Promers and a Gene Probe for Extensive Detection of Poly(3-hydroxybutyrate) (PHB)-degrading Bacteria Possessing Fibronectin Type III Linker Type-PHB Depolymerases"Appl. Microbiol. Biotechnol.. 801-806 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sano, D., Fukushi, K., Yano, K., Yoshida, Y., Omura, T.: "Enhanced Virus Recovery from Municipal Sewage Sluge with a Combination of Enzyme and Cation Exchange Resin"Water Science and Technology. Vol. 43, No. 2. 75-82 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, T., Sano, D., Omura, T.: "Risk Evalation for Pathogenic Bacteria and Viruses in the Compost of Sewage Sludge"Proc. of 1st International Water Association (IWA) Asia-Pacific Regional Conference. 729-734 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imachi, H., Sekiguchi, Y., Kamagata, Y., Ohashi, A., Harada, H.: "Cultivation and In Situ Detection of a Thermophilic Bacterium Capable of Oxidizing Propionate in Syntrophic Association with Hydrogenotropnic Methanogens in a Thermophilic Methanogenie Granular Sludge"Applied and Environmental Microbiology. 3608-3615 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekiguchi, Takahashi, H., Y., Kamagata, Y, Ohashi, A., Harada, H.: "In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sluges: a Novel Isolate Affiliated with a Clone Cluster, the Green Non-Sulfur Bacteria, Subdivision I"Applied and Environmental Microbiology. 5740-5749 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirata, A. Hashimoto and M.Z.B. Alam: "Quantitative Risk Assessment of Cryptosporidium in a Watershed, Advances in Water and Wastewater Treatment, Edited by Matsuo, T., Hanaki, K., Satoh, H."Elsevier. 91-99 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Alam, M. Z. B., Ohgaki, S.: "Evaluation of UV-radiation and its residual effect for algal growth control, Advances in Water and Wastewater Treatment, Edited by Matsuo, T., Hanaki, K., Satoh, H."Elsevier. 109-117 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katayama H., M. Nakamura and S. Ohgaki: "PCR determination of inactivated RNA colipnage Qβ, Advances in Water and Wastewater Treatment, Edited by Matsuo, T., Hanaki, K., Satoh, H."Elsevier. 101-108 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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