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2002 Fiscal Year Final Research Report Summary

Gene Detection by Light-to-electric Converting Intercalator and Its Application to DNA

Research Project

Project/Area Number 12305054
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 工業分析化学
Research InstitutionKyushu University

Principal Investigator

TAKAGI Makoto  Faculty of Engineering, Prof., 大学院・工学研究院, 教授 (90037739)

Co-Investigator(Kenkyū-buntansha) NOJIMA Takahiko  Faculty of Engineering Research Associate, 大学院・工学研究院, 助手 (00291930)
NAKAMURA Shigeo  Miyazaki Univ., Faculty of Engineering, Assoc. Prof., 工学部, 助教授 (00264078)
TAKENAKA Shigeori  Faculty of Engineering, Assoc. Prof., 大学院・工学研究院, 助教授 (60188208)
Project Period (FY) 2000 – 2002
Keywordsphotocurrent / gene detection / DNA chip / intercalator / ruthenium complex / threading intercalation
Research Abstract

In this research we developed a novel detection system of DNA duplex using a light-to-electric conversion. The principle of the detection of DNA duplex formation is as follows. 1) The gold electrode modified by single stranded DNA was prepared. 2) When double stranded DNA is formed, ruthenium complex will intercalate and will be concentrated on the electrode surface. 3) Then the visible light is irradiated to the electrode, photocurrent caused by excitation of ruthenium complex will be observed.
We used a polypyridyl ruthenium complex carrying DNA intercalating ligand for DNA detection. Ru(bpy)_3 was used as a control compound. Ru(bpy)_2dppz was used as a ruthenium complex carrying a DNA intercalating part. Furthermore, we synthesized NDI-Ru_2 as a threading intercalator carrying ruthenium complex. This type of compound has a smaller dissociation rate from DNA duplex, because it has threading intercalating part connected to two bulky groups
A DNA-immobilized gold electrode was dipped into the solution containing the light-to-electric converting intercalator (Ru(bpy)_3, Ru(bpy)_2dppz, NDI-Ru_2). When the visible light was irradiated to the surface of the electrode, continuous photocurrent occurred in the presence of tertiary amine as an electron donor. The amount of photocurrent increased by 36% in the case of Ru(bpy)_2dppz, 86% in the case of NDI-Ru_2 after the formation of double-stranded DNA. This result suggested that the metallointercalator was concentrated on the surface of gold electrode by its affinity to DNA duplex. In the case of NDI-Ru_2, we consider that electron was smoothly transferred from ruthenium to gold electrode through naphthalenediimide because NDI acts as an electron acceptor. In conclusion, we could discriminate between double stranded and single stranded DNA from the magnitude of the photocurrent using a ruthenium intercalator.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] B.Juskowiak, et al.: "Fluorescence energy transfer study of interstrand DNA cross-linking caused by rigid bisintercalator"Supramolecular Chemistry. 14. 477-485 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Nakamura, et al.: "Light-to-electric conversion as a new method for DNA duplex detection"Analytical Sciences. 17. 431-432 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] B.Juskowiak, et al.: "Isomerization of DNA-bound distilbazolium ligand induced by electron transfer photoexcited tris(1,10-phenanthroline)Ru(II)"Photochemistry and Photobiology. 74. 391-400 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Kumasaki, et al.: "A cyclic sugar unit interacts more strongly with DNA than do open-chain polyol coun erparts in sugar-linked synthetic DNA intercalators"Chemistry Letters. 2001. 370-371 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] B.Mathew, et al.: "Lipophilic peptide as novel sensory material for chiral amine recognition"Chemistry Letters. 2000. 1029-1030 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kuboyama, et al.: "Lipophilic peptide as novel building material of molecular sensor"Chemistiy Letters. 2000. 552-553 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] B.Juskowiak, et al.: "Fluorescence energy transfer study of interstrand DNA cross-linking caused by rigid bisintercalator"Supramolecular Chemistry. 14. 477-485 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Nakamura, et al.: "Light-to-electric conversion as a new method for DNA duplex detection"Analytical Sciences. 17. 431-432 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] B.Juskowiak, et al.: "Isomerization of DNA-bound distilbazolium ligand induced by electron transfer photoexcited tris(1, 10-phenanthroline)Ru(II)"Photochemistry and Photobiology. 74. 391-400 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Kumasaki, et al.: "A cyclic sugar unit interacts more strongly with DNA than do open-chain polyol counterparts in sugar-linked synthetic DNA intercalators"Chemistry Letters. 370-371 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] B.Mathew et al.: "Lipophilic peptide as novel sensory material for chiral amine recognition"Chemistry Letters. 1026-1027 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kuboyama et al.: "Lipophilic peptide as novel building material of molecular sensor"Chemistry Letters. 552-553 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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