2001 Fiscal Year Final Research Report Summary
Modecular design of artificial glycoconjugate polymers for developing biotedrnology
| Project/Area Number |
12305059
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
高分子合成
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
KABAYASHI Kazukiyo Nagoya U. Engineering. Prof., 工学研究科, 教授 (10023483)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIURA Yoshiko Nagoya U. Engineering. Associate Prof., 工学研究科, 助手 (00335069)
NISHIDA Yoshihiro Nagoya U. Engineering. Associate Prof., 工学研究科, 助教授 (80183896)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Keywords | Carbohydrate / Biomaterials / Polymer / Molecular Recognition / DNA |
|---|
| Research Abstract |
A complex of pTRI-β-actin plasmid DNA-lactose conjugate with β-galactose-specific RCA_<120) lectin is proposed as a functional mimic of the chromatin structure comprising DNA and histones : the in vitro transcription of DNA in the conjugate with T7 RNA polymerase was repressed in the presence of RCA_<120> and then the transcription ability was recovered by adding lactose or a lactose-carrying polymer to the repression system. The complex can be regarded as an artificial regulation system of transcription.
In conclusion, we have demonstrated the first successful regulation of gene expression with an artificial recognition system using a combination of a plasmid-canpohydtate conjugate with lectin. The concept proposed in this paper has possibility to be extended to various ligand-receptor systems and will open up new dimensions in biomedical researches.
|
