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2003 Fiscal Year Final Research Report Summary

Project study of tissue regeneration with tissue engineering

Research Project

Project/Area Number 12307051
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

YAMADA Satoru  Tokyo Dental College, Dept.Dentistry, Professor, 歯学部, 教授 (20103351)

Co-Investigator(Kenkyū-buntansha) MAEDA Katumasa  Kyusyu University, Dept.of Dental Science, Professor, 大学院・研究科・口腔機能修復学, 教授 (00117243)
TAKASHIBA Shogo  Okayama University, Dept of Periodontology, Professor, 大学院・医歯学総合研究科・歯周病態学, 教授 (50226768)
KURIHARA Eiji  Hiroshima University, Dept of Periodontology, Professor, 大学院・歯周病態学分野, 教授 (40161765)
ODA Shigeru  Tokyo Medical and Dental University, Dept.of Periodontology, 大学院・歯学研究科・歯周病学, 講師 (70160869)
HASEGAWA Kouji  Showa University, Dept.of periodontology, Professor, 歯学部, 教授 (70014024)
Project Period (FY) 2000 – 2003
Keywordstissue regeneration / tissue engineering / Proliferating tissue / human periodontal ligament / gene profiling / bFGF / SPARC / neurotrophin
Research Abstract

1. Mesenchymal stem cells : Proliferating tissue in periodontal osseous defects promotes the formation of new periodontal tissue around the root(Yamada.Ota). We find that the extracellular matrix is a critical regulator of the induction of alkaline phosphatase and the formastion of multiple layered in human gingival fibroblast(Maeda). One clone, PDL-29, identified as a COX assembly factor, showed much stronger m RNA expression in HPEs than in HGFs in culture. (Takashiba)
2. Signaling molecules :
Placement of PGS between the root surface and rhBMO-2 3 PGS complex had the effect to decrease ankylosis(Kawanami). SPARC plays arole in repair of periodontal tissues by promotingproliferation and osteoclastogenesis inhibitory factor production(Kurihara). EMDOGAIN has a potential to promote the cementum regeneration and to create a favorable environment that will promote the periodontal regeneration. CPC seemed to act as a scaffold for bone formation and histocompatible healing of periodontal tis … More sues(Oda). PGE2, cAMP-elevating agent, EP2/EP4 agonist stimulated cAMP accumulation in HPDL cells. However, BMP-2 hand no effect on it, and per-treatment with BMP-2 also did not cause significant changes in the cAMP accumulation stimulated with PGE2 or EP2 / EP4 agonist(Hasegawa). LJE becomes shorter, whereas the proliferative activity of regenerative connective tissue maintains the same level of proliferation, and ultimately LJE is replaced by regenerative connective tissue(Hashimoto9.Topical application of 0.1-0.4% bFGF induced significant periodontal tissue regeneration in animal experiments. In vitro studies demonstrated that bFGF stimulation in the presence of fetal calf serum inhibited proliferation of gingival epithelial cells and induced the release of hyaluronan and heparan sulfate from periodontal ligament cells (Murakami)
3. Scaffolds : The PLLA membrane showed an excellent results in comparison with the PLGA membrane in vitro experiment. Moreover, the histological observation showed no different bone regeneration in dogs in between PLLA and PLGA membrane(Izumi). The effect of matrix geometry upon the periodontal reconstruction induced by BMP was studied. This study using geometrically different cell substrata demonstrated that a matrix with a certain geometrical size is most favorable for cell differentiation((Takida) Less

  • Research Products

    (55 results)

All Other

All Publications (55 results)

  • [Publications] 石川烈, 白方良典, 小田茂: "新世紀の組織再生材料"日本歯科医学会雑誌. 22. 24-30 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小田茂, 石川烈: "エムドゲインを用いた再生治療"東京都歯科医師会雑誌. 51. 3-11 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石川烈, 小田茂 監著: "クインテッセンス出版"歯科医のためのスケーリング・ルートプレーニング. 134 (2003)

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      「研究成果報告書概要(和文)」より
  • [Publications] 木下淳博, 小田茂: "医歯薬出版"歯周病治療のストラテジー. 395 (2002)

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  • [Publications] 石川烈, 小田茂, 小島丈尚: "永末書店"周組織再生とエナメルタンパク. 149 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] Takayama, S. et al.: "Effects of basic fibroblast growth factor on human gingival epithelial cells."J.Periodontol. 73・12. 1467-1473 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murakami, S. et al.: "Recombinant human basic fibroblast growth factor (bFGF)stimulates periodontal regeneration inclass II furcation defects created in beagledogs"J.Periodont.Res. 38・1. 97-103 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 村上伸也: "塩基性線維芽細胞増殖因子による歯周組織再生の試み"大阪大学歯学雑誌. 47・2. 75-84 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimono, M. et al.: "Regulatory mechanisms of periodontal regeneration. In "The biology of tooth regeneration""icrosc Res Tech.. 60. 491-502 (2003)

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      「研究成果報告書概要(和文)」より
  • [Publications] Shimono, M. et al.: "Biological characteristics of the junctional epithelium."J.Electron Microsc.. 52. 627-639 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Usuda, J. et al.: "Proliferative activities of epithelial and connective tissue cells in the rat periodontal regeneration using AgNORs staining."J.Priodont Res.. In printing. (2004)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kuboki, Y., Kikuchi, M., Takita, H., Yoshimoto, R., Nakayama, Y., Matsuda, T., Ikada, Y.: "Laser-perforated membranous biomaterials induced pore size-dependent bone induction when used as a new BMP carrier. Connect. Tissue Res."Connect.Tissue Res.. 44. 318-325 (2003)

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      「研究成果報告書概要(和文)」より
  • [Publications] Takita, H., Kikuchi, M., Sato, Y., Kuboki, Y: "Inhibition of BMP-induced ectopic bone formation by an antiangiogenic agent(epigallocatechin 3-gallate)."Connect.Tissue Res.. 43. 529-534 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuboki, Y., Jin, Q.M., Takita, H.: "Geometry of carriers controlling phenotype expression in BMP-induced osteogenesis and chondrogenesis."J.Bone Joint Surg. 83-A. 105-114 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] 編者:内山茂, 小田茂, 鈴木哲也, 須田英明, 田上順次, 豊島義博: "いま注目の歯科器材・薬剤2002 歯科展望別冊"医歯薬出版. 186 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kikuchi M, Takita H, Nakayama Y, Matsuda T, Kuboki Y: "Laser-perforated collagen membrane : pore size-dependent bone induction of a new BMP carrier.J.Hard Tissue"Biol.. 9. 79-89 (2000)

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  • [Publications] 啓明, 滝田裕子, 渥美公則, 向後隆男, 久保木芳徳: "BMP誘導異所骨形成におけるヒドロキシアパタイト細胞支持体の幾何学的要素"Hokkaido J.Dent.Sci.. 21. 32-41 (2000)

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  • [Publications] Q.M., Takita, H., Kohgo, T., Atsumi, K., Itoh, H., Kuboki, Y.: "Effects of geometry of hydroxyapatite as a cell substratum."J.Hard Tissue Biol.. 10. 116-122 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Uno, K., Abiko, Y., Takita, H., Ukegawa, T., Satoh, N., Ueda, Y., Ohata, N., Kaku, T., Kuboki, Y.: "Effects of mechanical stress on the expression of type X II collagen mRNA in human periodontal ligament cells."J.Hard Tissue Biol.. 10. 116-122 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ukegawa, T., Takita, H., Uno, K., Sato, N., Ueda, Y., Ohata, N., Mizuno, M., Kuboki, Y.: "Chemical response of periodontal ligament cells toward rhBMP-2"J.Hard Tissue Biol.. 10. 108-115 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tang, M., Takita, H., Kohgo, T., lizuka, M., Doi, Y., Kuboki, Y.: "BMP-induced osteogenesis with two geometrically different biodegradable carbonate apatites."J.Hard Tissue Biol.. 10. 7-16 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuboki, Y., Jin, Q.M., Takita, H.: "Geometry of carriers controlling phenotype expression in BMP-induced osteogenesis and chondrogenesis.J."Bone Joint Surg.. 83-A. 105-114 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Mahmood, J., Takita, H., Ojima, Y., Kobayashi, M., Kohgo, T., Kuboki, Y.: "Geometric effect of matrix upon cell differentiation : BMP-induced osteogenesis using a new bioglass with a feasible structure."J.Biochem.. 129. 163-171 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kuboki, Y., Takita, H., Mizuno, M., Fujisawa, R.: "Geometry of artificial extracellular matrices : a new paradigm from dental tissue engineering."Dentistry in Japan. 37. 42-50 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kuboki, Y., Takita, H., Mizuno, M., Fujisawa, R.: "Geometry of artificial extracellular matrices : a new paradigm from dental tissue engineering."Dentistry in Japan. 37. 42-50 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, Y., Takita, H., Ohata, N., Tamura, M., Kuboki, Y.: "Pleiotrophin regulates bone morphogenetic protein(BMP)-induced ectopic osteogenesis."J.Biochem.. 131. 877-886 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuboki, Y., Jin, Q.M., Kikuchi, M., Mahmood, J., Takita, H.: "Geometry of artificial ECM: Sizes of pores controlling phenotype expression in BMP-induced osteogenesis."Connect.Tissue Res.. 43. 529-534 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takita, H., Kikuchi, M., Sato, Y., Kuboki, Y.: "Inhibition of BMP-induced ectopic bone formation by an antiangiogenic agent( epigallocatechin 3-gallate)."Connect.Tissue Res.. 23. 215-229 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Vehof, J.W.M., Takita, H., Kuboki, Y., Spauwen, P.H.M., Jansen, J.A.: "Histological characterization of the early stages of bone morphogenetic protein-induced osteogeneisis."J.Biomed.Mater Res.. 61. 440-449 (2002)

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  • [Publications] 宮田康一, 飯塚 正, 横山敦郎, 滝田裕子, 森田 学, 向後隆男: "骨形成蛋白箪Fよる異所性骨形成へのビタミンCの影響.Hokkaido"J.Dent.Sci.. 23. 215-229 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuboki, Y., Kikuchi, M., Takit., Yoshimoto, R., Nakayama, Y., Matsuda, T., Ikada, Y.: "Laser-perforated membranous biomaterials induced pore size-dependent bone induction when used as a new BMP carrier.Connect."Tissue Res.. 44(Suppl.1). 318-325 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 久保木芳徳, 滝田裕子: "骨形成蛋白質(BMP):その構造と新機能"Clinical calcium. 13. 467-471 (2003)

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  • [Publications] Kuboki, Y., Fujisawa, R., Mizuno, M., Li, D., Takita, H.: "From biomineralization to hard tissue engineering. Biomineralization : formation, diversity, evolution and application."Proceedings of the 8^<th> Interantional Symposium on Biomineralization.(Eds.) Kobayashi, I. and Ozawa, H.. (in press).

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  • [Publications] Kato, Y., Morita, M., Takita, H., Fujisawa, R., Iijima, M., Osawa, T., Kuboki, Y.: "Solubilization of organic matrix of Lingula unguis with chitinase. Biomineralization : formation, diversity, evolution and application."Proceedings of the 8^<th> International Symposium on Biomineralization.(Eds.) Kobayashi, I.and Ozawa, H.. (in press).

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  • [Publications] Tatsuya Abe, Yukiko Abe, Yoshitomi Aida, Yoshitaka, Hara, Katsumasa Maeda.: "Extracellular matrix regulate induction of alkaline phosphatase expression by ascorbic acid in human fibroblasts"J.Cell.Physiol. 189(2). 144-151 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Myokai F, Oyama M, Nishimura F, Ohira T, Yamamoto T, Arai H, Takashiba S, Murayama Y: "Unique genes induced by mechanical stress in periodontal ligament cells."Journal of Periodontal Research. 38(3). 255-261 (2003)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Mineshiba F, Takashiba S, Mineshiba J, Matsuura K, Kokeguchi S, Murayama Y: "Antibacterial activity of synthetic human β defensin-2 against periodontal bacteria.."Journal of the International Academy of Periodontology. 5(2). 35-40 (2003)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Fujimoto C, Maeda H, Kokeguchi S, Takashiba S, Nishimura F, Arai H, Fukui K, Murayama Y: "Application of denaturing gradient gel electrophoresis(DGGE) to the analysis of microbial communities of subgingival plaque."Journal of Periodontal Research. 38(4). 440-445 (2003)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto T, Myokai F, Nishimura F, Ohira T, Shiomi N, Yamashiro K, Aral H, Murayama Y, Takashiba S: "Gene profiling in human periodontal ligament fibroblasts by subtractive hybridization."Journal of Dental Rsearch. 82(2). 641-645 (2003)

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  • [Publications] Shimono M, Ishikawa T, Ishikawa H, Matsuzaki H, Hashimoto S, Muramatsu T, Shima K, Inoue T.: "Regulatory mechanisms of periodontal regeneration."Microsc Res Tech.. 60(5). 491-502 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimono M, Ihikawa T, Enokiya Y, Muramatsu T, Matsuzaka K, Inoue T, Abiko Y, Yamaza T, Kido MA, Tanaka T, Hashimoto S.: "Biological characteristics of the junctional epithelium."J Electron Microsc.. 52(6). 627-639 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Usuda J., Hashimoto S., Enokiya Y., Inoue T., Shimono M.: "Proliferative activities of epithelial and connective tissue cells in the rat periodontal regeneration using AgNORs staining."J.Periodont.Res.. (in printing). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takayama S, Yoshida J, Hirano H, Okada H, Murakami S.: "Effects of basic fibroblast growth factor on human gingival epithelial cells.."J.Priodontol.. 73(12). 1467-1473 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murakami S, Takayama S, Kitamura M, Shimabukuro Y, Yanagi K, Ikezawa K, Saho T, Nozaki T, Okada H.: "Recombinant human basic fibroblast growth factor (bFGF) stimulates periodontal regeneration in class II furcation defects created in beagle dogs"Journal of Periodontal Research. 38(1). 97-103 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 村上伸也: "塩基性線維芽細胞増殖因子による歯周組織再生の試み"大阪大学歯学雑誌. 47(2). 75-84 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 菊池重成: "ラット歯周組織欠損にエナメルマトリクスデリバティブタンパクを塗布した隙にみられる歯周組織治癒の実験的研究"口腔病学会雑誌. 68(1). 1-11 (2001)

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  • [Publications] Shirkata Y, Odas, Kinoshita A, Kikuchi S, Tuchioka H, Ishikawa I: "Histocompatible healing of periodontal defects after application of an inreactable calcium phosphate bone cement. A preliminary study in dogs."Journal of Periodontology.. 73(9). 24-30 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 小田 茂, 石川 烈: "エムドゲインRを用いた再生治療"

    • Description
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  • [Publications] 杉戸博記: "ePTFE膜を用いて形成された組織の歯槽骨およびセメント質再生能に関する研究"東京都歯科医師会雑誌. 51(10). 3-11 (2003)

    • Description
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  • [Publications] 難波 崇: "ePTFE膜を用いて形成された組織における歯周組織再生能の経時的変化に関する研究"日歯周誌. 41(3). 238-251 (1999)

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  • [Publications] 宮地裕史, 菅谷 勉, 宮本哲朗, 加藤勝也, 加藤 熈: "Hard tissue formation on dentin surface applied with recombinant human bone morphogenetic protein-2 in the connective tissue of the palate."日歯周誌. 43(3). 374-387 (2001)

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  • [Publications] 西村浩司, 小田島朝臣, 川浪雅光: "rhBMP-2による歯周組織再生と骨性癒着抑制法の長期的評価"日本歯周病学会誌. 44(4). 376-386 (2002)

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  • [Publications] Tsuyoshi Fujita, Hideki Shiba, Masatoshi Sakata, Yuushi Uchida, Shigeo Nakamura, Hidemi Kurihara: "SPARC stimulates of OPG/OCIF, MMP-2 and DNA in human periodontal ligament cells."J Oral Pathol Med.. 31. 345-352 (2002)

    • Description
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  • [Publications] Tsuyoshi Fujita, Hideki Shiba, Masatoshi Sakata, Yuushi Uchida, Tetsuji Ogawa, Hidemi Kurihara: "Effects of transforming growth factor-B, and fibronectin on SPARC expression in cultures of human periodontal ligament cells."Cell Biology International.. 26(12). 1065-1072 (2002)

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  • [Publications] Hidemi Kurihara, Hiroaki Shinahara, Hiroshi Yoshino, Katsuhiro Takeda, Hideki Shiba: "Neurotriphins in cultured cells from periodontal tissues.."J Periodontol.. 74(1). 76-84 (2003)

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Published: 2005-04-19  

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