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2007 Fiscal Year Final Research Report Summary

Development of anti-tick vaccine

Research Project

Project/Area Number 12356008
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

ONUMA Misao  Hokkaido University, Graduate School of Vet.Med., Professor (70109510)

Co-Investigator(Kenkyū-buntansha) SUGIMOTO Chihiro  Obihiro Universityof Agricul.and Vet.Med., Res.Center for Protozoan Mol.Immunol., Professor (90231373)
OHASHI Kazuhiko  Hokkaido University, Graduate School of Vet.Med., Associate Professor (90250498)
HATAKEYAMA Hideki  Bayer Co.Ltd., 研究開発事業部, Research Project Leader
Project Period (FY) 2000 – 2003
Keywordstick / anti-tick vaccine / recombinant antigen / Pathogens tramsmission inhibition / anti-blood feeding / serpin
Research Abstract

Maintenance of mammalian homeostatic balance is a multi-faced process involving an interaction of many proteins, however the need for a balance and coordinated interaction of many proteins, however the need for a balance and coordinated interaction between members of the serine proteinase super family and their inhibitors, known as serpins occupy a central role. In mammalian systems where extensive data exists, both serine proteinases and serpins are known to regulate such important functions as the blood coagulation cascade, fibrinolysis, food digestion, fertilization, inflammatory and immune responses. Imbalances or deficiencies in function of either serine proteinases or serpins have resulted in diseases. Ticks encode proteinases or serpins to disrupt the host homeostatic balance to their advantage.
For this reason, we examined the molecular cloning of tick-encoded serpins as a candidate for tick vaccine. By using rapid amplification of the cDNA (RACE method), two genes (HLS l and HLS 2) from H. longicornis were cloned. Both recombinant HLS l and 2 showed the anti-coagulant activities. Vaccinaion of rabbits with either recombinant HLS 1 or 2 expressed in Escherichia coli resulted in significant anti-tick immunity.
At present, we have characterized 4 tick salivary gland proteins whose recombinant products were effective against tick. We are planning to use these 4 cocktail vaccine for prevent the transmission of pathogens.

  • Research Products

    (9 results)

All 2004 2003

All Journal Article (9 results)

  • [Journal Article] Cloning, expression and partial characterization of a Haemaphysalis longicornis and a Rhipicephalus appendiculatus glutathione S-transferases.2004

    • Author(s)
      da Silva Vaz.
    • Journal Title

      Insect Biochem. Mol. Biol. 13

      Pages: 329-335

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A serine proteinase inhibitor (serpin) from ixodid tick Haemaphysalis longicornis; cloning and preliminary assessment of its suitability as acandidate for a tick vaccine.2003

    • Author(s)
      Sugino M.
    • Journal Title

      Vaccine 21

      Pages: 2844-2851

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Passive immunization with monoclonal antibodies : effects on Haemaphysalis longicornis tick infestation of BALB/c mice.2003

    • Author(s)
      Nakajima M.
    • Journal Title

      Jpn. J. Vet. Res. 50(4)

      Pages: 157-163

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Production and characterization of monoclonal antibodies against midgnt of ixodid tick Haemaphysalis longicornis.2003

    • Author(s)
      Nakajima M.
    • Journal Title

      Vet. Parasitol. 115

      Pages: 355-363

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Four Serine proteinase inhibitor (serpin) from brown ear tick, Rhipicephalus appendiculatus: cDNA cloning and preliminary characterization.2003

    • Author(s)
      Mulenga A.
    • Journal Title

      Insect Biochem. Mol. Biol. 33

      Pages: 267-276

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Three serine proteinases from midgets of the hard tick Rhipicephalus appendiculatus: cDNA cloning and preliminary characterization.2003

    • Author(s)
      Mulenga A.
    • Journal Title

      Exp. Applied. Acarology 29

      Pages: 151-164

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A serine proteinase inhibitor (serpin) from ixodid tick Haemaphysalis longicornis; cloning and preliminary assessment of its suitability as a candidate for a tick vaccine.2003

    • Author(s)
      Sugino M.
    • Journal Title

      Vaccine 21

      Pages: 2844-2851

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Four serine proteinase inhibitor (serpin) from brown ear tick, Rhipicephalus appendiculatus: cDNA cloning and preliminary characterization.2003

    • Author(s)
      Mulenga A.
    • Journal Title

      Insect Biochem. Mol. Biol. 33

      Pages: 267-276

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Three serine proteinase from midgets of the hard tick Rhipicephalus appendiculatus: cDNA cloning and preliminary characterization.2003

    • Author(s)
      Mulenga A.
    • Journal Title

      Exp. Applied. Acarology 29

      Pages: 151-164

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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