2002 Fiscal Year Final Research Report Summary
Development of novel humanized-mice and application to regenerative medicine
| Project/Area Number |
12357002
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Human pathology
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| Research Institution | National Research Institute for Child Health and Development (2001-2002)
Keio University (2000) |
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Principal Investigator |
HATA Jun-ichi National Research Institute for Child Health and Development, Head (90051614)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Jun-ichiroh National Research Institute for Child Health and Development, Dept.Developmaent and differentiation, Director
YAMADA Taketo Keio Unicersity School of Medicine, Dept. Pathology, Instructor (60230463)
KIYOKAWA Nobutaka National Research Institute for Child Health and Development, Dept.Developmaent and differentiation, General manager
UMWZAWA Akhiro National Research Institute for Child Health and Development, Dept.Reproduction, Director (70213486)
SAKURADA Kazuhiro National Research Institute for Child Health and Development, Kyowa Hakko Kogyo Co.Ltd., Tokyo Institute, Researcher
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| Project Period (FY) |
2000 – 2002
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| Keywords | Regeneration / Osteoporisis / tumor angiogenesis / hetatitis / heart muscle / bone marrow stroma / cell transplantation |
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| Research Abstract |
Application of cells/tissues obtained from a human as a new therapy for the disease attracts attention. It seems to be logical that cells/tissues obtained from patients are used for therapy because there is not rejection. Therefore it is important that multiply various cells out of a body are induced into certain differentiation via a specific course. For developmental research of new medical material, we examined an introduction of differentiated cells derived from human bone marrow mesenchymal stem cells and inoculation of these human cells into immunodeficiency mice in this study.
At first, the differentiation of cardiac muscle cells from human bone marrow mesenchymal stem cells which cells are easy to gather was performed. As a result, the method for differentiation into the cardiac muscle cells from human marrow mesenchymal stem cells was established. These obtained cardiac muscle cells may be useful for treatment of irreversible heart disorder such as myocardial infarction or cardiomyopathy. Furthermore it succeeds that the human cardiac muscle cells were transplanted into hearts of NOD/SCID. This experimental system may be available for preclinical trial of these muscle cells in vivo and evaluation of safety.
The translantations of human bone or liver into NOD/SCID mice were examined. As a results, the reproduction of human bone remodeling, hemopoietic cells and blood vessels derived from human bone was observed. Human hepatic tissues also were maintained in murine tissues over 4 months. These humanized mice (SCID-hu) may be available for drug screening, preclinical trials of novel therapies including gene therapy or molecular targeting therapy, or in vivo analysis in molecular pathology.
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[Journal Article] The use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly DL-lactic- co-glycolic acid(PLGA)-collagen sponge.2003
Author(s)
Ochi,K., Chen,G, Ushida,T., Gojo,S., Segawa,K., Tai,H., Ueno,K., Ohkawa,H., Mori,T., Toyama,Y., Hata,J, Umezawa,A.
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Journal Title
J.Cell. Physiol 194
Pages: 45-53
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] The use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly DL-lactic- co-glycolic acid(PLGA)-collagen sponge2003
Author(s)
Ochi, K., Chen, G., Ushida, T., Gojo, S., Segawa, K., Tai, H., Ueno, K., Ohkawa, H., Mori, T., Toyama, Y., Hata, J, Umezawa, A.
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Journal Title
J.Cell.Physiol. 194
Pages: 45-53
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Brain from Bone : Efficient "meta-differentiation" of marrow stroma-derived mature osteoblasts to neurons with noggin ora demethylating agent.2001
Author(s)
Kohyama,J., Abe,H., Shimazaki,T., Koizumi,A., Nakashima,K., Gojo,S., Taga,T., Okano,H., Hata,J., Umezawa,A
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Journal Title
Differentiation 68
Pages: 235-244
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
[Journal Article] Brain from Bone : Efficient "meta-differentiation" of marrow stroma-derived mature osteoblasts to neurons with noggin ora demethylating agent2001
Author(s)
Kohyama, J., Abe, H., Shimazaki, T., Koizumi, A., Nakashima, K., Gojo, S., Taga, T., Okano, H., Hata, J., Umezawa, A.
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Journal Title
Differentiation 68
Pages: 235-244
Description
「研究成果報告書概要(欧文)」より
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