2002 Fiscal Year Final Research Report Summary
Generation of Wolfram syndrome mice, aiming at development of new therapeutics for diabetes through preserving pancreatic beta cells
| Project/Area Number |
12357007
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Metabolomics
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| Research Institution | Tohoku University (2001-2002)
Yamaguchi University (2000) |
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Principal Investigator |
OKA Yoshitomo Tohoku University Graduate School of Medicine, Division of Molecular Metabolism and Diabetes, Professor, 大学院・医学系研究科, 教授 (70175256)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Kazuma Tohoku University Hospital, Department of Diabetes and Metabolism, Research Associate, 医学部附属病院, 助手 (60292215)
HINOKIO Yoshinori Tohoku University Hospital, Department of Diabetes and Metabolism, Research Associate, 医学部附属病院, 助手 (10282071)
KATAGIRI Hedeki Tohoku University Graduate School of Medicine, Division of Advanced Therapeutics for Metabolism and Diabetes, Professor, 大学院・医学系研究科, 教授 (00344664)
ISHIHARA Hisamitsu Tohoku University Hospital, Department of Diabetes and Metabolism, Research Associate, 医学部附属病院, 助手
HIRAI Masashi Tohoku University Hospital, Department of Diabetes and Metabolism, Research Associate, 医学部附属病院, 助手 (80312578)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Diabetes / Insulin / pancreatic β cell / Wolfram syndrome / WFS1 / endoplasmic reticulum / knock-out mice |
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| Research Abstract |
Wolfram syndrome is an autosomal recessive disorder associated with juvenile onset diabetes mellitus, optic atrophy, sensorineural deafness and diabetes insipidus. We identified, employing positional cloning, a gene responsible for this disorder and designated it WFS1. We also showed WFS1 protein to be localized in the ER membrane. In addition, WFS1 shares some sequence similarity with yeast Hrd3p, which is involved in ER-associated degradation of malfolded proteins. Thus, though WFS1 protein appears to play a role in the ER-stress response, its precise function remains to be clarified. To elucidate the functions of this novel protein and the pathophysiology of Wolfram syndrome, we have generated mice lacking the WFS1 gene (WFS1-KO). WFS1-KO mice are normal in appearance, growth and fertility Blood glucose levels in male WFS1-KO mice start to rise at around 12 weeks and are significantly higher than those of wild-type mice at 24 weeks (267±33 vs 146±34 mg/dl). This is associated with loss of islet β cells; whole pancreatic insulin content in WFS1-KO mice was 30-times lower than that in wild-type mice (11±4 vs 287±34 ng/mg pancreas). Immunohistochemical analysis of the pancreas using an anti-WFS1 protein antibody revealed that WFS1 protein is absent from exocrine tissue, but is strongly expressed in endocrine β cells. WFS1 protein is not expressed in glucagon-positive cells and the majority of somatostatin-, and pancreatic polypeptide-positive cells are devoid of WFS1 immunoreactivity. In WFS1-KO mice, β cell number fell with age, while a cells were somewhat increased and were scattered throughout the islet. These results strongly suggest WFS1 protein to play a critical role in survival and/or regeneration of β cells and progressive loss of islet β cells to be the major cause of diabetes in this disorder.
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Research Products
(12 results)
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[Publications] Tanizawa Y, Nakai K, Sasaki T, Anno T, Ohta Y, Inoue H, Matsuo K, Koga M, Furukawa S, Oka Y: "Unregulated elevation of glutamate dehydrogenase activity induces glutamine-stimulated insulin secretion : Identification and characterization of a GLUDi gene mutation, and insulin secretion studies with MIN6 cells overexpressing the mutant GDH"Diabetes. 51. 712-717 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Cryns K, Thys S, Van Laer L, Oka Y, Pfister M, Van Nassauw L, Smith RJ, Timmermans J-P, Van Camp G: "The WFS1 gene, responsible for low frequency sensorineural hearing loss and Wolfram syndrome, is expressed in a variety of inner ear cells"Histochem Cell Biol. 119. 247-256 (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] Cryns K, Thys S, Van Laer L, Oka Y, Pfister M, Van Nassauw L, Smith RJ, Timmermans J-P, Van Camp G: "The WFS1 gene, responsible for low frequency sensorineural hearing loss and Wolfram syndrome, is expressed in a variety of inner ear cells."Histochem Cell Biol 119. 247-256 (2003)
Description
「研究成果報告書概要(欧文)」より
