2002 Fiscal Year Final Research Report Summary
A basic research for use of hepatocyte growth factor (HGF) for diagnosis and treatment of periodontal disease.
| Project/Area Number |
12357011
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
DAIKUHARA Yasushi Kagoshima University, Dental School, Professor, 歯学部, 教授 (40028733)
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| Co-Investigator(Kenkyū-buntansha) |
KAJIHARA Takehiro Kagoshima University, Dental School, Research Associate, 歯学部, 助手 (10305138)
TAMURA Masato Hokkaido University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (30236757)
MACHIGASHIRA Miho Kagoshima University, Dental School, Research Associate, 歯学部, 助手 (80253897)
KAKIMOTO Kyoko Kagoshima University, Dental School, Research Associate, 歯学部, 助手 (40274849)
SUGIYAMA Akiko Kagoshima University, Dental School, Research Associate, 歯学部, 助手 (90304534)
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| Project Period (FY) |
2000 – 2002
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| Keywords | hepatocyte growth factor (HGF) / scatter factor / (SF) / periodontal disease / gingival crevicular fluid / HGF-activator (HGFA) / highly sensitive ELISA for HGF |
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| Research Abstract |
Hepatocyte growth factor (HGF), also known as scatter factor, is a broad-spectrum and multifunctional cytokine and is shown to be involved in the development, growth and regeneration of various organs and tissues. The expression of HGF in human gingival fibroblasts in induced by inflammatory cytokines such as interleukin 1. Thus, it is possible that content of HGF in gingival crevicular fluid (GCF) in periodontal disease is increased but this has not so far been reported because the volume of GCF is too small to determine HGF. Recently, we developed a highly sensitive enzyme-linked immunosorbent assay (ELISA) for HGF, with a detection limit of 1 pg/ml sample. Using this ELISA, we found that the mean HGF content in GCF from sites with clinically healthy gingiva was 1.7 ng/ml, and that of periodontitis was 3.23 ng/ml. Since the mean level of HGF in serum from healthy subjects is 0.2 ng/ml, our results indicate that HGF is synthesized and activated in gingival tissue. We confirmed that HGF is expressed by gingival fibroblasts and inflammatory cells by in situ hybridization. We also showed that HGF-activator, which converts inactive pro-HGF to active mature HGF, is expressed in gingival tissue by immunostaining and reverse transcription-polymerase chain reaction. These results suggest that HGF may be useful for diagnosis and treatment of periodontal disease.
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[Publications] R. Warn, P. Harvey, A. Warn, A. Folney-Comer, P. Heldin, M. Versnel, N. Arakaki, Y. Daikuhara, C. J. Laurent, S. E. Sarah, E. Herrick and S. E. Mutsaers: "HGF/SF induces mesothelial cell migration and proliferation by autocrine and paracrine pathways."Exp. Cell Res.. 267. 258-266 (2001)
Description
「研究成果報告書概要(欧文)」より
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